Vol. 16—1962 - NorthEastern Weed Science Society

64
OP AtmtO mIAZOLE
ONTHEGERMINATION OP NORTHERN NUTmASSSEEDI
THEE~~OT Op. FOLIAR:.APptICATION~
Jr. It. HUl, ~1. H. 'Lachman, D. N. l1aynard
and W. C. Lincoln, Jr. 2 "
University of Massachusetta Agricultural Expedment Station
Amherst, Massachusetts
Introduction
Donnalley and Ilahn (3) have reported that they applied Cl4
labeled amino triazole to the leaves of the Northern nutgrass plant.
Autoradiograms of treated plants ahatTedthat the chemical was trans· ,
located in'the plant and into the tubera. Subsequeae'tests showed
that this treatment reduced cermination of the tubers.
The object of this experiment was to detemine whether foliar
applications of amino triazole are translocated to the inflorescence
and if this treatment has an affect on seed germiaatiOn.
}~terials and 118thods
Plants of Northern nut grass (~eru8 esculencu I
) were erown
in the ereenhouse and treated with~4 labeled 3 am no-l,2,4
triazole; the tacced atom was on the S-position of the triazole
ring. The specific activity of .this chemical waa 0.9S millicuries
per millimole. A 4500 ppm stock solution, contaiatngSO microcuries,
was prepared by.addinz distilled water to the sample.
Each treatment consisted of a ten microliter droplet, with an
activity of 0.5 microcurie., beins placed inside a: lanolin ring on
the plant.~eactiv1ty was aimUar :lD all 1;&8e8,.but the site of
appl1catioD and the duration of the treatment wa..... r:Led. Five
microliters of a 0.1 per cent TritoD B-19S6 spreader .olution was
added to each droplet.
At the end of the various treatment periods,ithe Unolin was
removed with absorbent tissue.. 1heaerial po~tion of the plant
was harvested, sectioned, placed be~~een blotters, bound in a
lThe project was financed in pert by fun4l!1fr.omN(tftheastern Regional
Project NB-42, "Studies of the Life Hf.story of tio~t:ilern Nutgrsss
(Cxperus e.sculeDtu,) as Related to Possible l1ethoda of Control".
2Th~ authors wish to thank lomchemProducts, Inc; ~.upplyin8 the
Cl4 labeled amino triazole. '
Contribution No. 1337. }~ssachu8etts Agricultural Experiment Station.