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Vol. 16—1962 - NorthEastern Weed Science Society

Vol. 16—1962 - NorthEastern Weed Science Society

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236<br />

Table 7 Iffect of 4ial,8110n the iab:lblUOD<br />

.--------- ... -_ ...... _._ ... -- r-- • • • ,...... • • • • - - ..,~_.. " ... - ... _ ....<br />

,-rcent<br />

Percent<br />

a~natlon iDbl~~tj.on<br />

....-..__.~-.~..-._-_._......•.<br />

Diaijzed·<br />

·10<br />

Non d~a11zed 95<br />

_.__..-.-_<br />

-_..-..---<br />

_.- _- _---,-------- .•..._-_ -.-<br />

As a result of this expertment aDd stability to heat.fur~ber extractions were<br />

made by placing autoclaved lIleal in dialyzer. tubing aDd eXtracting in a Soxhlet<br />

extractor. thus extracting and dialyzing in one operati'Ott.·<br />

Ion excMne;e<br />

Table 7 shows the inhibitor is not absorbed to either cation or anion<br />

exchaqeredcs e-. JlIe~e was very. little 19S5 of act~Yitlon passage throuah<br />

Amberlite lR..l20 oratt,e.r havil16 passed t!;irpugl1 bot~ ,t:1;1isresin aDd AIIlberll,te<br />

lRA./too. .... . . . .. .' ' :<br />

Tabl.8<br />

----.-. __.----.~.~~~---.._~-----~-~~.-_.._-_..<br />

Perc.nt,!.~ent<br />

germination<br />

inhibition<br />

-- -_._ _.~-- •. _ •• • • .•..... • • .• -,"!' •.. .,-~ - ••• _ ..<br />

Through AIIlberlite<br />

m-120<br />

Through AIIlberlite<br />

IM-4QO. .<br />

o<br />

o<br />

100<br />

•.. _._. ~O.o<br />

J'.J<br />

Paper<br />

chrO!l!&togIaphY<br />

Deionized and dialyzed extract frOID 5 g.Att:s-a~lgliris was streaked<br />

on one 19 x 19 8&8 470..A filter paper having a Wba .;lfo. 7 wick. The solvent<br />

used was isopropanol water (90..1o-v/,,» 1nan~.~phere. Discs t..ere cut<br />

out of the chromatograph with a cork borer and placed in 5 ml. beake ..a. Each<br />

disk was wetted with .5 ml. water aDd 5 seeds were added for assay. Mostof<br />

the inhibtor was found between Rf values 56-72 (Figure I). This was aheadof<br />

_thy! red which bad a Rf value of about 47.

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