BSL PRO Software Guide - Biopac

78 Part CStandard Curve SettingsStandard Curve Settings sets parameters for the Standard Curve to be plotted. If a curve has been previously plotted, an alert isgenerated and the graph is immediately updated to reflect the new settings.Plot From/To: Defines the range of Y-axis values for the data points to beplotted. The default range is from 0 to 1 units. Enter new range values toexpand or limit the points on your list that are referenced when plotting thecurve.Plot using: Sets the resolution, or number of samples, of the plotted curve.The default is 100.When plotting Standards, use: Allows control of dot color and size of thestandard points on the list to be plotted. Choose from the pull-down menu toenter a new setting. (Note: The color of the standard curve is assigned on theX-axis channel, selected by right-clicking in the graph and choosing theColor option.)Setting up a file to plot a Standard Curve1. Launch BSL PRO to a new BSL PRO file with default parameters.2. Choose MP menu >Setup Channels and set CH1 and CH2 for Acquire Data and Plot on Screen.3. Change the Label for Channel 1 to “Concentration” and change Scaling units to “mg/100 ml.”• Click View/Change Parameters for Channel 1 and change the channel Label to “Concentration.”• Click Scaling and change the Units label to “mg/100 ml.”• Click OK to accept the new scaling parameters.• Click OK again to close out of the Input Channel Parameters dialog.4. Change the Label for Channel 2 to “Absorbance” and change Scaling units to “A.”• Click View/Change Parameters for Channel 2 and change the channel Label to “Absorbance.”• Click Scaling and change the Units label to “A.”• Click OK to accept the new scaling parameters.• Click OK again to close out of the Input Channel Parameters dialog.5. Close the Setup Channels dialog.6. Press Start and then Stop in the graph window to acquire a small amount of data.7. Go to the X/Y display mode by clicking its icon on the Toolbar.8. Establish “Concentration” for the X-axis and “Absorbance” for the Y-axis.• Click in the X-axis label displayed above the waveform window and choose Ch1, Concentration for the X-axis.• Click in the Y-axis label displayed to the left of the waveform window and choose Ch2, Absorbance for the Y-axis.9. Scale the X-axis (Horizontal Scale) and Y-axis (Vertical Scale) appropriately for your experiment.• For example, set the “Absorbance” scale to go from 0 to 1, and set the “Concentration” scale to go from 0 to 100mg/100 ml. Your actual scale settings will depend on your experiment.• To set the scales, click in the horizontal and vertical scale regions, respectively.Setup is now complete and you are ready to plot the curve.Visit the online support center at www.biopac.com