Mechanisms of aluminium neurotoxicity in oxidative stress-induced ...

CHAPTER 3
Table 1. In vitro effects of the presence of aluminium on the enzyme activities of SOD,
GPx, CAT, and MAO
Al 3+ (�M) SOD (U) GPx (U) CAT (U)
MAO
(nmol 4-HQ/mg protein/min)
MAO-A MAO-B
control 10.8 � 0.86 1.96 � 0.194 24.7 � 1.77 1.15 � 0.077 2.19 � 0.057
10 11.2 � 0.78 2.14 � 0.131 22.8 � 0.42 1.14 � 0.054 2.15 � 0.064
50 11.3 � 0.41 2.25 � 0.115 23.4 � 1.43 1.13 � 0.083 2.17 � 0.089
100 11.0 � 0.92 2.17 � 0.257 24.0 � 2.75 1.14 � 0.095 2.16 � 0.052
SOD, GPx, and CAT activities were determined using commercial enzymes, while
MAO activity (MAO-A and MAO-B) was determined using mitochondrial preparation
obtained from rat brain. All the incubations were performed at 37ºC. Abbreviations:
SOD, superoxide dismutase; GPx, glutathione peroxidase; CAT, catalase; MAO,
monoamine oxidase; 4-HQ, 4-hydroxyquinoline. Data are expressed as means � SD
from four determinations (n = 4). Significance of differences among groups was
assessed by a one-way ANOVA followed by a Bonferroni‟s test. No significant
differences were obtained for p < 0.05.
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