ARTIFICIAL INSEMINATION IN FARM ANIMALS - Phenix-Vet

Artificial Insemination in Swine 103are accomplished by employees of the farms. This condition reinforces our position that theIUAI technique can be accomplished at commercial farms by the own employeesresponsible for the gestation sector.InseminationtechniqueSpermatozoidnumberFarrowing rate( 1 n)Estrusrepetition rateNumber of newbornsby farrowingIntracervical 3x10 9 /100 mL 90.0 (54) 10.0 11.5 ± 3,4Intrauterine 1x10 9 /100 mL 93.3 (56) 6.7 11.7 ± 3,4Intrauterine 1x10 9 /50 mL 86.7 (52) 13.3 11.4 ± 3,2Intrauterine 5x10 8 /100 mL 93.3 (56) 6.7 11.8 ± 3,0Intrauterine 5x10 8 /50 mL 90.0 (54) 10.0 11.4 ± 3,6Table 6. Farrowing, estrus repetition rates and total piglets born per farrowing in eachinsemination technique (60 females per treatment). 1 n: Number of animals which gave birthaccording to each insemination technique. No differences (P>0.05) occurred between theinsemination techniques for farrowing and estrus repetition rates by the chi-square test. Nodifferences occurred (P>0.05) between the insemination techniques for number of newbornsby farrowing using Kruskal – Wallis test. Adapted from Araújo et al. (2009).2.3 Deep intrauterine artificial and intratubal InseminationsIn the last years, many researches concerning to the deep intrauterine insemination (IUPAI)have been accomplished. In this technique, a low insemination volume (5mL) is used as wellas reduced concentration of spermatozoids (200 million), without the need for surgicalintervention (Vazquez et al., 2000). The objective of the researches accomplished until themoment is to turn this technique applicable (not endanger the reproductive efficiency of theherd, so that it can be commercially implanted at large scale. In addition, the use of reducedvolume and low concentration of the sperm in IUPAI will favor the use of frozen semenand/or sexed.The reduction of the semen volume used in IUPAI rather guarantees the optimization of theboar, as providing economical advantages to the farms. The possibility for using thistechnique is in line with the needs imposed by modern swine raise, which looks forreducing the insemination dose under use. This would provide a reduction in the malebreeding stock and even in the frequency of using these ones.It is considered that a great number of the spermatozoids are lost in ICAI process (Martínezet al. 2001). This occurs due to the semen reflux as well as to spermatozoid phagocytosis bythe polymorphonuclear leucocytes. It is believed that approximately 1/3 of thespermatozoids by backflow in 2 hours after AI, due to those physiologic processes (Viring &Einarsson, 1981). After overcoming those obstacles, approximately 1 X 10 3 spermatozoidscan be rescued at the caudal portion of the isthmus, a place where the spermatic cell staysuntil ovulation to occur (Mburu et al., 1996).In this context, the IUPAI objective is the reduction of the spermatic flow inside the uterus,as reducing the seminal reflux and the phagocytosis rate on those cells (Vazquez et al.,2008). In addition, some physical barriers are transposed as the cervical folds andendometrial crypts. Thus, the insemination dose under use could be significantly reduced.So, Martínez et al. (2002) verified that IUPAI with 5 x 10 7 spermatozoids by dose (5mL)presents no differences in the gestation and parturition rates neither in the size of the litter,when compared with ICAI by using 3 x 10 9 spermatozoids (100mL). However, it is