ARTIFICIAL INSEMINATION IN FARM ANIMALS - Phenix-Vet

Evaluation of a New Method and Diagnostic Test in Semen Analysis 145A1.00.8sensitivity0.60.40.2c=160 M/ml, AUC=0.960c=200 M/ml, AUC=0.922c=240 M/ml, AUC=0.8850.00.0 0.2 0.4 0.6 0.8 1.01 - specificity (false positive rate)B1.00.8sensitivity0.60.40.2SI=140 M/ml, AUC=0.903SI=180 M/ml, AUC=0.922SI=220 M/ml, AUC=0.9480.00.0 0.2 0.4 0.6 0.8 1.01 - specificityFig. 10 (A, B). ROC plots of resazurin reduction assay for identifying semen samples withpre-selected minimum concentration of motile sperm concentration (A) and motile andnormal sperm (B).However, in clinical use of the test, it is often important to 100% correctly identifysatisfactory or unsatisfactory samples. Therefore, a cut-off value of A 610 at 0.342 was selectedto enable 100% correct identification of unsatisfactory semen samples. For both criteria, thetest is 100% sensitive at A 610 of 0.342. A cut-off value at A 610 of 0.121 gives 100% specificityfor motile sperm concentration and 95.8% specificity for SI. For pre-selected minimumconcentration of motile sperm concentration of 160×10 6 sperm/mL and SI of140×10 6 sperm/mL, 100% specificity was obtained at the optimal cut-off value of A 610 at0.254, whereas only moderate levels of sensitivity were observed (80.6% and 73.5%,respectively; Figures 11A and 12A). In contrast, at the highest criteria values 100%sensitivity corresponded to only moderate levels of specificity (Figures 11C and 12C). Incontrast, semen samples with A 610 below 0.121 in the resazurin reduction assay were 100%