ARTIFICIAL INSEMINATION IN FARM ANIMALS - Phenix-Vet

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70Artificial Insemination in Farm AnimalsForsberg et al., 1999; Thomassen et al., 2006). The conception rates after intravaginalinsemination with frozen-thawed semen are significantly lower when compared with theresults of intrauterine insemination.5.4.2.1 The Norwegian or Scandinavian techniqueThe method of non-surgical transcervical intrauterine insemination was first time describedin 1975 (Andersen, 1975). The technique has been adapted from the artificial inseminationperformed in foxes. Two catheters are used in this method - the outer plastic catheter andinner metal thin catheter. There are 3 sizes of the catheters, for small, medium and largebreeds. The catheterisation should be made on standing animal. Sometimes there is no needfor administration of sedatives, but usually a small dose of alpha-mimetic, such asmedetomidine, is advisable for abdominal muscles relaxation. The outer plastic cathetershould be introduced into the vagina. It should be advanced as far as is possible. In manybitches, especially those of larger breeds, the tip of the catheter passes into the cranialnarrow part of vagina. However, in some smaller bitches the introduction of the outercatheter through the paracervix is difficult. It is necessary to palpate the end of the catheterand the cervix through the abdominal wall. The cervix is palpable at estrus as solid, ovoidstructure. It is advisable to move the tip of the catheter ventrally towards the ventral regionof abdominal wall. This procedure is helpful in palpation of the cervix. The inner metalcatheter should be introduced through the plastic catheter. The cervix should be fixedbetween the thumb and other fingers and tilted to horizontal axis. The metal catheter isintroduced into the cervical canal under the control of the position of the cervix by palpationthrough the abdominal wall (Andersen, 1975; Linde-Forsberg, 1991). This techniquedemands skill and experience. It is harder to perform uterine catheterisation in obese ornervous bitches and in giant breeds.The scandinavian method of uterine catheterization is recommended for routineinsemination of bitches (Ferguson et al., 1989; Günzel-Apel 1994; Linde-Forsberg, 1991,1995). The use of this technique of insemination is especially advisable in cases when usingof semen of lower quality due to male subfertility or sperm cryopreservation. Linde-Forsberg and Forsberg (1989) obtained 83.9% and 69.3% of pregnant bitches (data correctedfor the stage of estrus) after insemination with fresh and frozen-thawed semen, respectively.The litter sizes were lower by 23.3%, when frozen-thawed semen was used in comparison tofresh semen. Rota et al. (1999a) reported 25% higher pregnancy rate after intrauterine semendeposition when using scandinavian technique than after vaginal semen deposition. On thebasis of analysis of 327 inseminations Linde-Forsberg et al. (1999) concluded that successrate of scandinavian method and vaginal insemination with frozen-thawed semen was84.4% and 58.9, respectively. Niżański (2006) proved that results of vaginal inseminationwith frozen-thawed semen are significantly lower in comparison with fresh semen, in spiteof the use of modification of the technique of vaginal semen deposition, plasma additionand adjustment of the number of spermatozoa.5.4.2.2 Endoscope-assisted vaginoscopic method (New Zealand method)Intrauterine insemination of the bitch under the visual control of endoscopic equipment wasfirst time described by Wilson (Wilson, 1993, 2001), using a rigid endoscope – cystouretroscopeof the length 29 cm with diagnostic external sheath. The procedure is performedon the standing animal. Uterine catheterisation is made with the use of flexible catheterintroduced into the working channel of the endoscope. The endoscope is introduced into the
Artificial Insemination in Dogs 71cranial narrow part of the vagina, while a flexible catheter is introduced cranio-dorsal intothe external orifice of the cervical canal under the visual control performed through theendoscope. Usually it is not necessary to administer any sedatives.The results for the intrauterine deposition of frozen-thawed semen when using thistechnique are quite satisfactory (Table 12). Wilson (1993), with the use of frozen semen,refers a pregnancy rate and litter size 83.3% and 7.5 puppies per litter, respectively. Niżański(2005) obtained whelping rates of 68.7% and 27.8%, when frozen-thawed semen wasdeposited by intrauterine vaginoscopic method and by vaginal insemination, respectively.Results obtained by Linde-Forsberg et al. (1999) were poorer in comparison with theScandinavian method. However, vaginoscopic intrauterine insemination is currentlyconsidered as the practical, modern and useful tool in assisted reproductive techniques indogs which may become in the future the routine method of insemination.InseminationtechniqueIntravaginal,using aninfusion pipeteIntrauterine,using theendoscopen1816Whelping rate(nº femaleswhelped/inseminated)27.8 a(5/18)68.7 b(11/16)Litter sizeat birth(range)3.0 ±1.2 a(2-5)4.9 ±1.7 b(3-8)Litter sizeat weaning(range)2.6 ±0.9 a(2-4)4.6 ±1.7 b(2-8)Different superscripts in the same column indicate significant difference (p
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VIContentsChapter 9Evaluation of a
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ARTIFICIAL INSEMINATION IN FARM ANIMALS - Phenix-Vet

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