ARTIFICIAL INSEMINATION IN FARM ANIMALS - Phenix-Vet

More documents

Recommendations

Info

114Artificial Insemination in Farm AnimalsPodda, M. C. A.; Costa, E.P.; Pinheiro, R.W.; Vilela, C.G.; Paiva, F.P.; Carvalho, F.F.; Costa,A.H.A. Oxitocina no sêmen diluído de varrões. I – Influência nos aspectos físicos dosêmen e morfológicos dos espermatozóides Proceedings of Congresso Brasileiro deVeterinários Especialistas em Suínos (ABRAVES). pp. 343-344. Belo Horizonte, MinasGerais, Brasil, outubro, 1999.Rath, D. Low dose insemination in the sow – A Review. (2002). Reproduction of DomesticAnimals. v.37, pp. 201 – 205.Roppa, L. (September/October 2006). Perspectivas da produção mundial de carnes, 2006 a2030. Revista Pork World, Editora Paulínia, n. 34, pp. 16 – 27 , São Paulo, SP.Soede, N.M., Kemp, B. (1996). Timing of AI and ovulation in sows. Proceedings ofInternational Conference On Boar Semen Preservation,1996Spencer, K.W.; Purdy, P.H.; Blackburn, H.D.; Spiller, S.F.; Stewart, T.S.; Knox, R.V. (2010).Effect of number of motile, frozen-twawed boar sperm and number of fixed-timeinseminations on fertility in estrous-synchronized gilts. Animal Reproduction Science,Vol. 121, (July 2010), pp.259-266, doi:10.1016/j.anireprosci.2010.07.002.Steverink, D.W.; Soede, N.M., Bouwman, E.G. (1998). Semen backflow after inseminationand its effect on fertilization in sows. Journal of Reproduction and Fertility, v.111,p.165-171, 1998.Sumransap, P.; Tummaruck P.; Kunavongkrit, A. (2007). Sperm distribution in thereproductive tract of sows after intrauterine insemination. Reproduction DomesticAnimals, vol. 42, No.2, (2007), pp.113-117, ISSN 0936-6768.Tummaruk, P.; Sumransap, P.; Techakumphu, M.; Kunavongkrit, A. (2007). Distribution ofSpermatozoa and Embryos in the Female Reproductive Tract after Unilateral DeepIntra Uterine Insemination in the Pig. Reproduction Domestic Animals, vol. 42, No.6,(December 2007), pp.603-609, ISSN 0936-6768.Vazquez, J. L.; Martínez, E. A.; Vazquez, J. M.; Lucas, X.; Gil, M. A.; Parrilla, I.; Roca, J.(2000). Development of a non-surgical deep intrauterine insemination technique.Proceedings of International Conference on Sêmen Preservation, Beltsville, 2000.Vazquez, J.M.; Martinez, E.A.; Roca, J.; Gil, M.A.; Parilla, I.; Cuello, C.; Carvajal, G.; Lucas,X.; Vazquez, J.L. (2005). Improving the efficiency os sperm Technologies in pigs: thevalue of deep intrauterine insemination. Theriogenology. Vol. 63, No.2 (January2005), pp.563-547. doi:10.1016Vazquez, J.M., Martinez, E.A., Roca, J., Gil, M.A., Parrilla, I., Cuello, C., Carvajal, G., Lucas,X., Vazquez, J.L., (2005). Improving the efficiency of sperm technologies in pigs: thevalue of deep intrauterine insemination. Theriogenology, Vol. 63, pp. 536–547.Vazquez, J.M.; Roca, J.; Gil, M.A.; Cuello, C.; Parrilla, I.; Vazquez, J.L.; Martínez, E.A.(2008). New developments in low-dose insemination technology. Theriogenology.Vol. 70, No. 8, (November 2008), pp. 1216-1224, DOI: 10.1016.Viring, S.; Einarsson, S. (1981) Sperm distribuition within the genital tract of naturallyinseminated gilts. Nordisch Veterinarian Medicine, v.33, p.145-149.Waberski D, Petrunkina AM, Töpfer-Petersen E. (2008). Can external quality controlimprove pig AI efficiency? Theriogenology. Vol. 70, No. 8, November, pp. 1346-51.Watson, P. F. & Behan, J. R. (2002). Intrauterine insemination of sow with reduced spermnumbers: results of a commercially based field trial. Theriogenology. Vol. 57, pp.1683 – 1693.Weber, D.; Bennemann, P.E.; Wentz, I.; Bortolozzo, F.P. (2003). Avaliação do Custo de DosesInseminantes Produzidas em Centrais de Inseminação Artificial de Suínos emSistema Fechado. Proceedings of XI Congresso Brasileiro de Veterinários Especialistas emSuínos (ABRAVES), Goiânia - GO, outubro, 2003.
Sperm Preparation Techniques forArtificial Insemination - Comparison ofSperm Washing, Swim Up, andDensity Gradient Centrifugation Methods7Ilaria NataliSterility Center, Gynecology and Obstetrics Unit,S.S. Cosma and Damiano Hospital, PistoiaItaly1. IntroductionThe Artificial Insemination (AI) is the first option treatment for infertile couples withcervical factor subfertility, mild-moderate male subfertility and unexplained infertility. Withthe exception of cases in which the use of in vitro fertilization (IVF or ICSI) is strictly due asa consequence of a severe male or female factor, the artificial insemination must be part of agradual approach to the techniques of artificial insemination. This is particularly the casesince the AI is a valid low-cost method, minimally invasive and easily acceptable for thefemale’s hormone treatment (Aribarg & Sukcharoen, 1995).The AI, as other assisted reproductive techniques, needs a selection of the ejaculatedspermatozoa before the performance of the treatment. In fact, some components of theseminal fluid may become an obstacle to the fertilization when the in vitro fertilization orthe intrauterine insemination are performed (Bjorndahl et al., 2005). Spermatozoa andleukocytes produce many oxygen radicals that alter the possibility of the sperm-oocytefusion after repeated centrifugations. So, the selection of the sperms from the othercomponents with methods like the swim up technique or the gradient density centrifugationmust be preferred (Aitken & Clarkson, 1988).Some different techniques are used to prepare the spermatozoa for the AI, but the choicestrongly depend on the quality of the semen, that is on the concentration, motility andmorphology, in order to obtain the higher number of good spermatozoa, even from thepoorest semens.The principle techniques of sperm preparation consist of migration, density gradientcentrifugation and filtration techniques. While for the migration the method is based onmovement of the spermatozoa, for density gradient centrifugation and filtration techniquesthe method is based on a combination of the motility and the retention at phase borders andadherence to filtration matrices, respectively (Henkel & Schill, 2003).The main techniques used for the AI are the sperm washing, the swim-up technique, and thedensity gradient centrifugation and they will be described as follow. The aim of the presentchapter is to shed light on the key principles and the best method for sperm selection inorder to obtain higher pregnancy rate.
Page 3 and 4:
VIContentsChapter 9Evaluation of a
Page 5:
XPrefaceless stress to the female,
Page 8 and 9:
2Artificial Insemination in Farm An
Page 10 and 11:
Page 12 and 13:
Page 14 and 15:
Page 16 and 17:
10Artificial Insemination in Farm A
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71: 64Artificial Insemination in Farm A
Page 106 and 107: 100Artificial Insemination in Farm
Page 170 and 171:
164Artificial Insemination in Farm
Page 172 and 173:
Page 174 and 175:
Page 176 and 177:
Page 178 and 179:
Page 180 and 181:
Page 182 and 183:
Page 184 and 185:
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 202 and 203:
Page 204 and 205:
Page 206 and 207:
Page 208 and 209:
Page 210 and 211:
Page 212 and 213:
Page 214 and 215:
Page 216 and 217:
Page 218 and 219:
Page 220 and 221:
Page 222 and 223:
Page 224 and 225:
Page 226 and 227:
Page 228 and 229:
Page 230 and 231:
Page 232 and 233:
Page 234 and 235:
Page 236 and 237:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
Page 244 and 245:
Page 246 and 247:
Page 248 and 249:
Page 250 and 251:
Page 252 and 253:
Page 254 and 255:
Page 256 and 257:
Page 258 and 259:
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274 and 275:
Page 276 and 277:
Page 278 and 279:
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
Page 288 and 289:
Page 290 and 291:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304 and 305:
Page 306:
show all

ARTIFICIAL INSEMINATION IN FARM ANIMALS - Phenix-Vet

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?