outcomes. A review of information published since1995. Cincinnati, OH: U.S. Department of Health<strong>and</strong> Human Services, Centers for Disease Control,National Institute for <strong>Occupational</strong> Safety <strong>and</strong>Health, DHHS (NIOSH) Publication No. 2011–172.NIOSH [2012]. General safe practices for workingwith engineered nanomaterials in research labora<strong>to</strong>ries.Cincinnati, OH: U.S. Department of Health<strong>and</strong> Human Services, Centers for Disease Control,National Institute for <strong>Occupational</strong> Safety <strong>and</strong>Health, DHHS (NIOSH) Publication No. 2012–147.Noll JD, Birch ME [2008]. Effects of sampling artifactson occupational samples of diesel particulatematter. Environ Sci Technol 42(14):5223–5228.NRC [2009]. Science <strong>and</strong> decisions: advancing riskassessment. Committee on Improving Risk AnalysisApproaches Used by the U.S. EPA, Board on EnvironmentalStudies <strong>and</strong> Toxicology, Division onEarth <strong>and</strong> Life Studies, National Research Councilof the National Academies. Washing<strong>to</strong>n, DC: TheNational Academies Press.Nurkiewicz TR, Porter DW, Barger M, MillecchiaL, Rao KM, Marvar PJ, Hubbs AF, Castranova V[2006]. Systemic microvascular dysfunction <strong>and</strong>inflammation after pulmonary particulate matterexposure. Environ Health Perspect 3(114):412–419.Nurkiewicz TR, Porter DW, Hubbs AF, S<strong>to</strong>ne S,Chen BT, Frazer DG, Boegehold MA, CastranovaV [2009]. Pulmonary nanoparticle exposuredisrupts systemic microvascular nitric oxide signaling.Toxicol Sci 1(110):191–203.Oberdörster G, Ferin J, Morrow PE [1992]. Volumetricloading of alveolar macrophages (AM): Apossible basis for diminished AM-mediated particleclearance. Exp Lung Res 18:87–104.Oberdörster G, Oberdörster E, Oberdörster J [2005b].Nano<strong>to</strong>xicology: an emerging discipline evolvingfrom studies of ultrafine particles. Environ HealthPerspect 113(7):823–839.Oyabu T, Myojo T, Morimo<strong>to</strong> Y, Ogami A, HirohashiM, Yamamo<strong>to</strong> M, Todoroki M, Mizuguchi Y,Hashiba M, Lee BW, Shimada M, Wang WN, UchidaK, Endoh S, Kobayashi N, Yamamo<strong>to</strong> K, FujitaK, Mizuno K, Inada M, Nakaza<strong>to</strong> T, Nakanishi J,Tanaka I [2011]. Biopersistence of inhaled MW-CNT in rat lungs in a 4-week well-characterizedexposure. Inhal Toxicol 23(13):784–791.Old L, Methner M [2008]. Effectiveness of localexhaust ventilation (LEV) in controlling engineerednanomaterial emissions during reac<strong>to</strong>r cleanou<strong>to</strong>perations. J Occup Environ Hyg 5(6):D63–D69.Olson DA, Norris GA [2005]. Sampling artifacts inmeasurement of elemental organic carbon: Lowvolumesampling in indoor <strong>and</strong> outdoor environments.Atmos Environ 39(30):5437–5445.OSHA, NIOSH [2011]. OSHA NIOSH infosheet:maximize your spirometry screening <strong>and</strong> surveillanceresources. OSHA Publication No. 3415-1-11. Washing<strong>to</strong>n, DC: <strong>Occupational</strong> Safety <strong>and</strong>Health Administration (OSHA) <strong>and</strong> Cincinnati,OH: National Institute for <strong>Occupational</strong> Safety <strong>and</strong>Health (NIOSH) [http://www.osha.gov/Publications/osha3415.html].Osmond-McLeod MJ, Pol<strong>and</strong> CA, Murphy F, Wadding<strong>to</strong>nL, Morris H, Hawkins SC, Clark S, AitkenR, McCall MJ, Donaldson K [2011]. Durability <strong>and</strong>inflammogenic impact of carbon nanotubes comparedwith asbes<strong>to</strong>s fibres. Part Fibre Toxicol 8:15.Pacurari M, Yin XJ, Zhao J, Ding M, LeonardSS, Schwegler-Berry D, Ducatman BS, Sbarra D,Hoover MD, Castranova V, Vallyathan V [2008].Raw Single-wall carbon nanotubes induce oxidativestress <strong>and</strong> activate MAPKs, AP-1, NF-kappaB, <strong>and</strong>Akt in normal <strong>and</strong> malignant human mesothelialcells. Environ Health Perspect 116(9):1211–1217.Pauluhn J [2010a]. Subchronic 13-week inhalationexposure of rats <strong>to</strong> multiwalled carbon nanotubes:Toxic effects are determined by density of agglomeratestructures, not fibrillar structures. Toxicol Sci113(1):226–242.Pauluhn J [2010b]. Multi-walled carbon nanotubes(Baytubes): approach for derivation of occupationalexposure limit. Regul Toxicol Pharmacol. [http://dx.doi.org/10.1016/j.yrtph.2009.12.012].84 NIOSH CIB 65 • <strong>Carbon</strong> <strong>Nanotubes</strong> <strong>and</strong> <strong>Nanofibers</strong>
Piegorsch WW, Bailer AF [2005]. Quantitative riskassessment with stimulus-response data. Chapter4. In: Analyzing Environmental Data. Chichester,West Sussex, Engl<strong>and</strong>: John Wiley <strong>and</strong> Sons.Pol<strong>and</strong> CA, Duffin R, Kinloch I, Maynard A, WallaceWA, Sea<strong>to</strong>n A, S<strong>to</strong>ne V, Brown S. Macnee W,Donaldson K [2008]. <strong>Carbon</strong> nanotubes introducedin<strong>to</strong> the abdominal cavity of mice showasbes<strong>to</strong>s-like pathogenicity in a pilot study. NatNanotechnol 3(7):423−428.Porter DW, Sriram K, Wolfarth M, Jefferson A,Schwegler-Berry D, Andrew M, Castranova V [2008].A biocompatible medium for nanoparticle dispersion.Nano<strong>to</strong>xicol 2(3):144–154.Porter DW, Wolfarth MG, Chen BT, McKinneyW, Hubbs AF, Battelli L, Andrews M, Frazer D,Castranova V [2009]. Pulmonary <strong>to</strong>xicity of inhaledmulti-walled carbon nanotubes. The Toxicologist108:A2193.Porter DW, Hubbs AF, Mercer RR, Wu N, WolfarthMG, Sriram K, Leonard SS, Battelli L, Schwegler-Berry D, Friend S, Andrew M, Chen BT, TsuruokaS, Endo M, Castranova V [2010]. Mouse pulmonarydose- <strong>and</strong> time course-responses induced byexposure <strong>to</strong> multi-walled carbon nanotubes. Toxicology269(2–3):136–147.Pulskamp K, Diabaté S, Krug HF [2007]. <strong>Carbon</strong>nanotubes show no sign of acute <strong>to</strong>xicity but induceintracellular reactive oxygen species in dependenceon contaminants. Toxicol Lett 168(1):58–74.Raabe OG, Al-Bayati MA, Teague SV, Rasolt A[1988]. Regional deposition of inhaled monodispersecoarse <strong>and</strong> fine aerosol particles in small labora<strong>to</strong>ryanimals. Ann Occup Hyg 32:53–63.Ramach<strong>and</strong>ran G, Ostraat M, Evans DE, MethnerMM, O’Shaughnessy P, D’Archy J, Geraci CL,Stevenson E, Maynard A, Rickabaugh K [2011].A strategy for assessing workplace exposures <strong>to</strong>nanomaterials. JOEH 8(11):673–685.Rappaport SM, Lyles RH, Kupper LL [1995]. An exposure-assessmentstrategy accounting for within—<strong>and</strong> between—worker sources of variability. AnnOccup Hyg 39(4):469–495.Rengasamy S, Verbofsky R, King WP, Shaffer RE[2007]. Nanoparticle penetration through NIOSHapprovedN95 filtering-facepiece respira<strong>to</strong>rs. J IntSoc Respir Protect 24:49–59.Rengasamy S, King WP, Eimer B, Shaffer RE [2008].Filtration performance of NIOSH-approved N95<strong>and</strong> P100 filtering-facepiece respira<strong>to</strong>rs against4-30 nanometer size nanoparticles. J Occup EnvironHyg 5(9):556–564.Rengasamy S, Eimer BC, Shaffer RE [2009]. Comparisonof nanoparticle filtration performanceof NIOSH-approved <strong>and</strong> CE-marked particulatefiltering facepiece respira<strong>to</strong>rs. Ann Occup Hyg53(2):117–128.Rengasamy S, Eimer BC [2011]. Total inward leakageof nanoparticles through filtering facepiece respira<strong>to</strong>rs.Ann Occup Hyg 55(3):253–263.Rom WN, Markowitz S [2006]. Environmental <strong>and</strong><strong>Occupational</strong> Medicine. 4th ed. Lippincott Williams& Wilkins.Rouse JG, Yang J, Ryman-Rasmussen JP, Barron AR,Monteiro-Riviere NA [2007]. Effects of mechanicalflexion on the penetration of fullerene aminoacid-derivatized peptide nanoparticles throughskin. Nano Lett 7(1):155–160.Ryman-Rasmussen JP, Riviere JE, Monteiro-RiviereNA [2006]. Penetration of intact skin by quantumdots with diverse physicochemical properties. ToxicolSci 91(1):159–165.Ryman-Rasmussen JP, Tewksbury EW, Moss OR,Cesta ME, Wong BA, Bonner JC [2009a]. Inhaledmultiwalled carbon nanotubes potentiate airway fibrosisin murine allergic asthma, Am J Respir CellMol Biol 40(3):349–358 [Epub ahead of print].Ryman-Rasmussen JP, Cesta MF, Brody AR,Shipley-Phillips JK, Everitt JI, Tewksbury EW,Moss OR, Wong BA, Dodd DE, Andersen ME,Bonner JC [2009b]. Inhaled carbon nanotubesNIOSH CIB 65 • <strong>Carbon</strong> <strong>Nanotubes</strong> <strong>and</strong> <strong>Nanofibers</strong>85
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CURRENT INTELLIGENCE BULLETIN 65Occ
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Current Intelligence Bulletin 65Occ
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ForewordThe Occupational Safety and
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Executive SummaryOverviewCarbon nan
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2009; Pauluhn 2010a; Porter et al.
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neurogenic sig nals from sensory ir
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possible. Until the results from an
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••Follow exposure and hazard as
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Periodic Evaluations••Evaluatio
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ContentsForeword ..................
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A.3.2 Comparison of Short-term and
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ESPFeFMPSFPSSgGMGSDHCLHECHEPAhrISOI
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AcknowledgementsThis Current Intell
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1 IntroductionMany nanomaterial-bas
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2 Potential for ExposureThe novel a
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CNMs, with MWCNT agglomerates obser
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composite materials with local exha
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information on air contaminants. Sa
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3 Evidence for Potential Adverse He
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decreasing agglomerate size increas
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examined up to 60 days post-exposur
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3.3 SWCNT and MWCNTIntraperitoneal
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The same potency sequence was obser
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Table 3-3. Findings from published
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Table 3-5. Findings from published
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Table 3-6. Findings from published
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Table 3-7 (Continued). Findings fro
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Table 3-8. Findings from published
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- Page 99 and 100: ReferencesACGIH [1984]. Particle si
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- Page 105 and 106: Kobayashi N, Naya M, Mizuno K, Yama
- Page 107 and 108: Methner M, Hodson L, Geraci C [2010
- Page 109: Human Services, Centers for Disease
- Page 113 and 114: AD, Baron PA [2003]. Exposure to ca
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or overloading, of particle clearan
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Table A-13. Human-equivalent retain
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A.7.1 Particle CharacteristicsBoth
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and density. The following MMAD and
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Table A-15. CNT lung dose normalize
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B.1 Key Terms Related toMedical Sur
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APPENDIX CNIOSH Method 5040
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filter. In the method evaluation, d
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Most of the studies on sampling art
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e analyzed to determine the onset o
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Delivering on the Nation’s promis