3rd International Poultry Meat Congress

O 39 Incidence of C. perfringens in Chicken Neck Samples Taken From
Slaughterhouse, Molecular Characterization and Definition of It’s
Antibiotic Resistance Profiles
Güzin İplikçioğlu Çil, Fatma Seda Bilir Ormancı
Ankara University, Faculty of Engineering, Department of Food Engineering, Ankara-Turkey
Abstract
The objectives of this study were, to determine the incidence of C. perfringens in 180 chicken
neck samples, to detect cpa gene by PCR for the verification of the isolates and to identify toxin
genes by multiplex PCR, to evaluate the seasonal distribution of C. perfringens prevalence,
and to find out the antibiotic susceptibility profiles of the isolates. C. perfringens was isolated
from 39 (21.6 %) of the 180 chicken meat samples by cultivation method. For the confirmation
of isolates, PCR assay was perfromed. From 39 isolates cpa gene were determined in 35 (89.7
%) of them. None of the isolates possesed the toxin genes cpb, cpb2, etx, iA and cpe. During
the winter (December, January, February) 13 and during the summer (June, July, August) 22
samples were found to be contaminated with C.perfringens so for the incidence of C. perfringens
seasonal difference was not significant for chi square test. According to the disc diffusion test
all of the isolates were found to be resistant at least one of the 9 antibiotics. 35 isolates was
resistant to trimetoprim (100 %). In addition, high level of resistance was also determined
against tetracycline (65,7 %) and gentamycin (62,8 %).
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