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Research Results - (PDF, 101 mb) - USAID

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Table 3. Ovulation rate and litter size in pure breeds and<br />

crossbreds<br />

Breed Group N % YMan Ovulation<br />

Rate<br />

Litter Size<br />

S 85 0<br />

DSXS 42 25<br />

F1 57 50<br />

F2 104 50<br />

DX DS 56 75<br />

D 70 100<br />

the underlying genetic basis of reproduction in<br />

these breeds. Table 3 summarizes ovulation rate<br />

and litter size of these two breeds and their<br />

various crosses.<br />

Both the ovulation rate and litter size<br />

increase in proportion to the increase in D'Man<br />

genotype in crosses, which suggests a quantitative<br />

inheritance.<br />

Two papers were accepted, and another<br />

one has recently been submitted, for publication<br />

in the Journal of Animal Science. A total of four<br />

papers were presented at the annual meeting of<br />

the European Association for Animal Production,<br />

and 4th World Congress on Genetics<br />

Applied to Livestock Production (two papers in<br />

each). A paper on repeatability estimates of<br />

litter size is in progress and will be submitted to<br />

Animal Reproduction Science.<br />

Complete citations for these papers are<br />

presented in the "Publications" section of this<br />

report.<br />

U.S.<br />

(Work supported primarily by the California<br />

Agr. Expr. Station but with some SR-CRSP<br />

support, e.g., for computing.)<br />

The transfer of frozen-thawed e<strong>mb</strong>ryos<br />

was highly successful as shown in table 4.<br />

1.06 1.03<br />

1.41 1.34<br />

1.73 1.49<br />

1.71 1.49<br />

2.31 1.85<br />

2.79 2.00<br />

Table 4. <strong>Results</strong> of an e<strong>mb</strong>ryo transfer study<br />

using frozen-thawed e<strong>mb</strong>ryos<br />

E<strong>mb</strong>ryo No. No. %Survival<br />

Classification Transferred La<strong>mb</strong>s<br />

Good 71 42 59.2<br />

Fair 28 13 46.4<br />

Poor 28 2 7.0<br />

Of e<strong>mb</strong>ryos classified as fair or good<br />

following thawing, 55% were represented as live<br />

born la<strong>mb</strong>s. However, survival rate of e<strong>mb</strong>ryos<br />

classified as poor was very low (7%), indicating<br />

that storing e<strong>mb</strong>ryos of poor quality at collection,<br />

or transferring e<strong>mb</strong>ryos which are morphologically<br />

abnormal on thawing is not justified.<br />

Most of the poor quality e<strong>mb</strong>ryos were of<br />

questionable quality when collected; a very high<br />

proportion which were satisfactory at freezing<br />

were also of acceptable quality after thawing,<br />

and the overall results indicate this is a good<br />

method of storing and transforming sheep germ<br />

plasm.<br />

An experiment on the "ram effect" in<br />

inducing out-of-season breeding was completed,<br />

and a paper has been prepared from the resulting<br />

M.S. thesis by W. Cushwa. It was determined<br />

that ewes joined with vasectomized rams<br />

two months prior to the start of the breeding<br />

season showed a higher response rate when<br />

joined with fertile rams. These ewes also<br />

la<strong>mb</strong>ed earlier than ewes that were completely<br />

isolated from rams until the regular breeding<br />

season. Data on ovulation rate and e<strong>mb</strong>ryo<br />

49

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