Gene regulation in Streptococcus pneumoniae - RePub - Erasmus ...
Gene regulation in Streptococcus pneumoniae - RePub - Erasmus ...
Gene regulation in Streptococcus pneumoniae - RePub - Erasmus ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Table 2. Oligonucleotide primers used <strong>in</strong> this study.<br />
Name primer Nucleotide sequence 5' - 3' (restriction sites are underl<strong>in</strong>ed) Restriction<br />
site<br />
Clon<strong>in</strong>g<br />
CodSacFwd GCGCGCCCGCGGAGGTCGTGCTGGTAAGTCAG SacII<br />
CodKpnRv GCGCGCGGTACCGATGATTTTCAGGCCAGATG KpnI<br />
CodH<strong>in</strong>dFwd<strong>in</strong>v TACGAAGCTTTGAGTCTGCGGGGATTATTG H<strong>in</strong>dIII<br />
CodPstRv<strong>in</strong>v ATGCCTGCAGGTGCCATTTTTCACCTCGAA PstI<br />
CodKOrv CATATTAGCCCCTTGAACGTAGTC -<br />
Cod 5' chrom CATGTCAAGATTGCGGCTAA -<br />
pcpA_L1 GTTTCCCAGAAAGACTCTGG<br />
pcpA_L2 a<br />
CCACTAGTTCTAGAGCGGCGGAGTATCTGCTAGGATTGG<br />
pcpA_R1 ATAAGAATACGGATTCGGACG<br />
pcpA_R2 a<br />
GCGTCAATTCGAGGGGTATCGATGGCTATCGAGTCAATGC<br />
pR412_L GCCGCTCTAGAACTAGTGG<br />
pR412_R GATACCCCTCGAATTGACGC<br />
Overexpression<br />
CodY-NheI-H6-Fw b CGGCTAGCCATCACCATCACCATCACGCACATTTATTAGAAAAAACT NheI<br />
CodYBamRv CGCGCGGGATCCTTAGTAATCTCTTTTCTT BamHI<br />
EMSA<br />
codY EMSA fw TGCAAGTCAATAAGGAATTTTCA -<br />
codY EMSA rv GCCATTTTTCACCTCGAATT -<br />
gdhA EMSA fw CCAAAAACTGAATTGAAAGAATTT -<br />
gdhA EMSA rv CTTTAGCAGATGTCATATCGTTCTCC -<br />
amiA EMSA fw GACACTTCGAACGACAATTTG -<br />
amiA EMSA rv TGACAACCATTATATCACATTATCCA -<br />
ilvB EMSA fw CATAAATAAACGTTAAAATAGAAAATTCAG -<br />
ilvB EMSA rv CCCTTTCTTTCCTCTTAAAAATAAC -<br />
ilvE EMSA fw GAAATGAAGAATCAGTTCTAAGATGG -<br />
ilvE EMSA rv TCCCAATCAATCGTTACTGTCA -<br />
livJ EMSA fw CCCTTTGTGGGCAATCTTTA -<br />
livJ EMSA rv CAAGCGCCACAAACGATA -<br />
psaR EMSA fw TGAAAGAAGAGCTATTTTCGTCAT -<br />
psaR EMSA rv CTTTGTTTGGGGTCATTCGT -<br />
livH EMSA fw TCAACGTCGCCTTGGATTAT -<br />
livH EMSA rv CGAGGGTTTTCCCTCACTTT -<br />
acuB EMSA fw TTCAGAGCTCTTTTTGCTAGCTT -<br />
acuB EMSA rv CCTTGCGGGTCATAAAATCT -<br />
asd EMSA fw CCCTAGTCTAGCGACTGGGATT -<br />
asd EMSA rv GCGCCGACTACAGCAACT -<br />
gapN EMSA fw CGCCTTGACGTAGTGGATTT -<br />
gapN EMSA rv TCGGATGATTTCCATTTTCC -<br />
aliB EMSA fw TTGAACAATCTTTTAGGAGAACTTGA -<br />
aliB EMSA rv CATTTCCAGAACCTCCTGCT -<br />
fatD EMSA fw TCCCTCGAAGATATTATTTTATCAGA -<br />
fatD EMSA rv TTTCATACCCCGTCCTTTCA -<br />
spr0140 EMSA fw GACATTCTATTTAGAACGAGGATTGA -<br />
spr0140 EMSA rv ATTCCCCCAGTTCCATTTTT -<br />
spr0141 EMSA fw TCTATCAAAATCGCAAATAAGAAA -<br />
spr0141 EMSA rv TTCCATTGTTTCTGCAAATTGT -<br />
spr0157 EMSA fw AACAATGTTTTAGAAGCAAAGGTG -<br />
spr0157 EMSA rv GCTTGCGACATGATAAATACTCC -<br />
spr0788 EMSA fw GAGGAAGGCCTTGTCCAGTT -<br />
spr0788 EMSA rv CCCATAGAGCAACCTGTCGT -<br />
The CodY regulon of S. <strong>pneumoniae</strong><br />
95<br />
95