Plant Variety Journal - IP Australia

Details of Application
Application Number 2006/122
Variety Name ‘DP 408 BGII’
Genus Species Gossypium hirsutum
Common Name Cotton
Synonym Nil
Accepted Date 29 Jun 2006
Applicant Deltapine Australia Pty Ltd, Narrabri, NSW
Agent N/A
Qualified Person Richard Leske
Details of Comparative Trial
Location
Descriptor Cotton (Gossypium) TG/88/6.
Period Field trial grown during the summer of 2006/07.
Conditions Field Trial Conditions: Plants grown from seed, each variety
grown on 1m row spacing x 12m plot length, commercial
rates of fertiliser, herbicides and insecticides applied as
required, trial fully irrigated. GMO Bio-assay conditions: leaf
disc samples removed from small plants and ground in
centrifuge tubes with extraction buffer, test strips
impreganated with antibodies added to detect for the presence
or absence of the Cry 1A(c) and Cry IIAb Bt proteins and the
RR herbicide protein.
Trial Design Randomised complete block with 10 replicates per variety.
Measurements Field trial: morphological plant characteristics measured from
10 non-tipped plants per replicate, one measurement per
plant. Fibre quality samples picked from a 1.5m section of
each row in each replicate and analysed by HVI instrument
testing. GMO bio-assay: leaf disc samples removed from 5
plants per replicate and tested for the presence or absence of
the Cry 1A(c) and Cry IIAb Bt proteins and for the absence of
RR herbicide protein.
RHS Chart - edition N/A.
Origin and Breeding
Controlled pollination: seed parent ‘DeltaEMERALD’ crossed with pollen parent ‘DP
50BX’ followed by 3 backcross cycles to the recurrent parent ‘DeltaEMERALD’. The
seed parent is the non GMO conventional recurrent parent variety and the pollen
parent is used to introduce the transgenic Cry 1A(c) and Cry2Ab Bt insect tolerance
genes. Hybridisation took place in Deltapine Australia’s greenhouse located at
Locharba, Narrabri, NSW. Single plants were selected under field conditions at
Locharba in the F3 generation. Progeny rows were selected in the F4 generation and
the final selection was tested in replicated yield and fibre quality trials from 2003/05.
Selection criteria included expression of the Cry1A(c) and Cry IIA Bt traits, disease
tolerance to bacterial blight and yield and fibre quality. Propagation: by seed.
Breeders: Richard Leske and Gerard Lonergan, Deltapine Australia Pty. Ltd,
Locharba, Narrabri, NSW.
Page 182 of 550
Plant Varieties Journal Vol. 21 No.3