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Plant Variety Journal - IP Australia

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Details of Application<br />

Application Number 2006/202<br />

<strong>Variety</strong> Name ‘Ruby’<br />

Genus Species Cannabis sativa<br />

Common Name Industrial Hemp<br />

Synonym Nil<br />

Accepted Date 15 Aug 2006<br />

Applicant Agri Fibre Industries Pty. Ltd. Bundaberg, QLD<br />

Agent N/A<br />

Qualified Person David Gillespie<br />

<strong>Plant</strong> Varieties <strong>Journal</strong> Vol. 21 No.3<br />

Details of Comparative Trial<br />

Location Bargara, QLD<br />

Descriptor Hemp (Cannabis sativa) TG/Hemp (DRAFT).<br />

Period 2008-2009.<br />

Conditions Trial 1 (Autumn 2008): the trial was sown in a well prepared red<br />

ferrisol kraznozem on 28th Feb 2008. Growth conditions were<br />

not conducive for plant measurements as we subsequently<br />

discovered that the area had previously been band treated with<br />

diuron two years before planting, too soon for a sensitive crop<br />

like industrial hemp, and this made the trial quite variable. Also<br />

the autumn time of sowing meant maturity was earlier and less<br />

spread out than the normal spring planting time. Leaf measures<br />

and plant heights were affected by the short day length and<br />

differences were slight compared to a spring planting. Weeds<br />

were removed at a young age by hand throughout the trial period.<br />

Watering was by trickle irrigation and plants were never stressed<br />

for water. Pest and diseases were controlled as necessary with<br />

insecticides and fungicides before there was any damage.<br />

Trial Design Randomised factorial complete block with 4 replicates with 100<br />

plants per variety per replicate.<br />

Measurements for leaf 30 samples per replicate, for plant height 20 samples per<br />

replicate.<br />

RHS Chart - edition 2001.<br />

Origin and Breeding<br />

Controlled pollination: the pollination was controlled to the extent that male plants were<br />

removed before anthesis from the maternal parent while several paternal parents of<br />

unknown origin to the applicant pollinated the maternal parent. The source F1 hybrid<br />

seed coded ‘Guelph 3-23’ was from very late maturing material (harvested 151 days from<br />

sowing) under Canadian conditions. The seed was imported through quarantine under<br />

licence from Mr Peter P. Dragla, now deceased, University of Guelph, Ridgetown<br />

College, Ontario, Canada in Dec 2003. The F1 source seed and subsequent generations<br />

were then grown in isolation until final selection. Individual plant selection for THC<br />

content is carried out in the first three generations where selected male plants open<br />

pollinate the female plants. Male plants are screened before anthesis and female plants<br />

screened at half seed fill using an in-house colorimetric test for THC content. The<br />

Government analyst also monitors populations with a random 30 plant sample. Short<br />

plants are discarded. <strong>Plant</strong>s are screened for bast fibre content by manual separation<br />

(whole stalk weighed and then bast fibre weighed and expressed as a percentage) after<br />

seed harvest. ‘Ruby’ measured 18% bast fibre content, while ‘Carmen’ measured 30%<br />

bast fibre content in previous experiments. Seed yields of individual plants are recorded<br />

with the highest yielding plants retained that have low THC content. Selection of grain<br />

types also involves taste tests at firm seed stage. Analysis of fatty acid content of good<br />

tasting seed is carried out by SGS Toowoomba, a NATA accredited laboratory.<br />

Page 235 of 550

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