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Plant Variety Journal - IP Australia

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Details of Application<br />

Application Number 2006/123<br />

<strong>Variety</strong> Name ‘DP 611 BGII/RR’<br />

Genus Species Gossypium hirsutum<br />

Common Name Cotton<br />

Synonym Nil<br />

Accepted Date 29 Jun 2006<br />

Applicant Deltapine <strong>Australia</strong> Pty Ltd, Narrabri, NSW<br />

Agent N/A<br />

Qualified Person Richard Leske<br />

Details of Comparative Trial<br />

Location Locharba, Narrabri, NSW.<br />

Descriptor Cotton (Gossypium) TG/88/6.<br />

Period Field trial grown during the summer of 2006/07.<br />

Conditions Field trial conditions: plants grown from seed, each variety<br />

grown on 1m row spacing x 12m plot length, commercial<br />

rates of fertiliser, herbicides and insecticides applied as<br />

required, trial fully irrigated. GMO Bio-assay conditions: leaf<br />

disc samples removed from small plants and ground in<br />

centrifuge tubes with extraction buffer, test strips impregnated<br />

with antibodies added to detect the presence or absence of the<br />

Cry 1A(c) and Cry IIAb Bt proteins and the RR herbicide<br />

protein.<br />

Trial Design Randomised complete block with 10 replicates per variety.<br />

Measurements Field trial: morphological plant characteristics measured from<br />

10 non-tipped plants per replicate, one measurement per<br />

plant. Fibre quality samples picked from a 1.5m section of<br />

each row in each replicate and analysed by HVI instrument<br />

testing. GMO bio-assay: leaf disc samples removed from 5<br />

plants per replicate and tested for the presence or absence of<br />

the Cry 1A(c) and Cry IIAb Bt proteins and the RR herbicide<br />

protein.<br />

RHS Chart - edition N/A<br />

Origin and Breeding<br />

Controlled pollination: F1 seed parent (‘DeltaPEARL’ x ‘DP 50 BX’) crossed with F1<br />

pollen parent (‘DeltaPEARL’ x ‘DP 555 BG/RR’) followed by 2 backcross cycles to<br />

the recurrent parent ‘DeltaPEARL’. The seed parent is used to introduce the<br />

transgenic Cry IIAb insect tolerance gene and the pollen parent is used to introduce<br />

the transgenic Cry 1A(c) insect tolerance gene and the Roundup Ready herbicide<br />

tolerance gene. Hybridisation took place in Deltapine <strong>Australia</strong>’s greenhouse located<br />

at Locharba, Narrabri, NSW. Single plants were selected under field conditions at<br />

Locharba in F3 generation. Progeny rows were selected in the F4 generation and the<br />

final selection was tested in replicated yield and fibre quality trials from 2002/05.<br />

Selection criteria included expression of the Cry1A(c) and Cry IIA Bt traits,<br />

expression of the Roundup Ready herbicide trait, disease tolerance to bacterial blight<br />

and yield and fibre quality. Propagation: by seed. Breeders: Richard Leske and Gerard<br />

Lonergan, Deltapine <strong>Australia</strong> Pty. Ltd, Locharba, Narrabri, NSW.<br />

Page 187 of 550<br />

<strong>Plant</strong> Varieties <strong>Journal</strong> Vol. 21 No.3

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