International Summer School PROGRAM - Laboratoire d'Infochimie ...

Supramolecular protein immobilization
A. González-Campo, J. Cabanas-Danes, L. Yang, D. Wasserberg, J. Huskens,
P. Jonkheijm*
Molecular Nanofabrication, University of Twente, P.O. Box 217, 7500 AE, Enschede,
Netherlands.
The immobilization of proteins onto surfaces is a continually growing area. 1 New
results to immobilize proteins, which are sensitive to remote electrochemical stimuli,
using cucurbituril (CB) and cyclodextrin (CD)-modified surfaces will be presented.
Site-selectively ferrocene-tagged fluorescent protein binds to self-assembled surfaces
through weak or strong interactions between the ferrocenyl guest and the host surface of
beta-CD or CB[7]. Upon reduction of the ferrocene unit, the protein was removed from
either surface, with the CD surface showing the fastest disassembly kinetics. The
stimuli-responsive attachment used will open new avenues for cell-responsive studies.