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2 ROLE OF SPACER IN THE PHASE-TO-PHASE LAYER OF POLYMERIC MICROSPHERES AT CONSTRUCTING OF BIOTEST-SYSTEMS 1 Fedorova O.V., 1 Zayarnyuk N.L., 2 Stanishevskiy Ya.M., 2 Gritskova I.A., 1 Novikov V.P. 1 National University “Lviv Politechnic” 79013, Lviv, Bandery str., 12, e-mail: vnovikov@polynet.lviv.ua Moscow State Academy of Fine Chemical Technology n. of M.V. Lomonosov When constructing biotest-systems on viral and infectious diseases specific immunoglobulins G (Ig G) have been used as bioligands. The immobilization of these immunoglobulins on the surface of functional polymeric microspheres proposed to through spacer, protein A (S. aureus). The choice of spaser is conditioned by its capability of the affine interaction with Fc - fragment of molecule. In this case Fab - fragment of Ig G provides more effective availability of binding to antigen’s determinants with potential causative agent of a disease. Optimal concentrations of protein A (0,05 mg/ml) on the surfaces of polymeric microspheres and minimum dilutions of serums, at which particles of conjugates "polymeric microsphere - protein A" spontaneously aggregate, have been determined. The orientation of Ig G molecule with hydrophilic Fab-fragment into the water phase results in lyophilization of a surface of a particle, which is the condition of the development of structural - mechanical factor by Rebinder. The creation of such type of diagnostic biotest-systems doesn’t require a preliminary selection and purification of specific molecules of Ig G, which are contained in γ- globulin’s fraction required. Stability of the conjugate "polymeric microsphere (PM) - protein A" allows using it more than 6 months. Biotest-systems of different specificity for the following diseases have been developed using highly titratable serums and universal conjugate "PM - protein A": yersiniosis (Y. enterocolitica); salmonellosis (S. pullorum); leptospirosis (L. conicula); for the chicken infectious bronchitis virus stamm Н120.
SYNTHESIS AND COMPLEXATION ABILITIES OF HYPERBRANCHED STRUCTURES BASED ON THIACALIX[4]ARENE Galukhin A.V., Mostovaya O.A., Antipin I.S., Stoikov I.I., Kazan Federal University, 18 Kremlyovskaya st., Kazan, e-mail: and_galuhin@mail.ru Hyperbranched macromolecules functionalized by polydental fragments are widely used for highly effective molecular recognition of various biologically relevant compounds, including biopolymers. Dendrimers based on p-tert-butylthiacalixarene have such interesting properties as high concentration of binding sites, highly developed surface and porosity. Thiacalixarene derivatives´ ability for molecular recognition of biologically relevant molecules´ surface is provided by conformational mobility of dendrimer´s “branches” and spatial organization of functional groups, forming complementary binding sites. In this work various dendrimers based on thiacalixarene in 1,3-alternate configuration were synthesized. Structures of obtained compounds were characterized by 1D and 2D NMR-spectroscopy, IR-spectroscopy, mass-spectrometry and elemental analysis. Complexation abilities of synthesized macrocycles with DNA of salmon sperm and calf thymus DNA were investigated by complex of spectroscopic methods: UV- and fluorescent spectroscopy, dynamic light scattering method. The financial support from Federal Program "Research and scientific-pedagogical cadres Innovative Russia" in 2009-2013 years (№ P1295 on 9 June 2010), RFBR (09- 03-00426, 10-03-92661-NSF) and the Program of the President of the Russian Federation for the State support of young Russian scientists - Doctors of Sciences (MD- 2747.2010.3) is gratefully acknowledged.
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4 th International Summer School Se
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4 th International Summer School
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Tuesday, September 13 th 9 - 10.30
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4.30 - 5.30 pm Poster Short Talks (
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2.30 - 4 pm Session VIII (Inorganic
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11.30 - 12.00 am Olga Federova, Mos
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INNOVATIVE DEVELOPMENTS ON THE BASI
Page 15 and 16: Calixarenes: from molecular recepto
Page 17 and 18: Controlled and Targeted Drug Delive
Page 19 and 20: What does the recognition of biomol
Page 21 and 22: Binding properties of calix[4]arene
Page 23 and 24: “Light and Molecular Design in Bi
Page 25 and 26: Supramolecular protein immobilizati
Page 27 and 28: Cucurbiturils at the interface betw
Page 29 and 30: Chiral Concave N-Heterocyclic Carbe
Page 31 and 32: Aromaticity Switching In Porphyrino
Page 33 and 34: Zwitterion-chromophore modules for
Page 35 and 36: Chemoinformatics approaches to virt
Page 37 and 38: Polyphosphorus Ligands in Supramole
Page 39 and 40: Lithium-controlled hierarchical ass
Page 41 and 42: Luisa De Cola Germany, Münster "Se
Page 43 and 44: Cationic amphiphilic calixarenes: s
Page 45 and 46: Supramolecular devices based on pho
Page 47 and 48: The sensing of interfacial interact
Page 49 and 50: Molecular recognition of organic qu
Page 51 and 52: Towards a molecular assembly based
Page 53 and 54: Analysis of benzodiaza-15-crown-5 e
Page 55 and 56: Crown-containing derivatives of nap
Page 57 and 58: Tuning of Knoevenagel-type substrat
Page 59 and 60: FRET based distance measurements on
Page 61 and 62: Synthesis and supramolecular associ
Page 63 and 64: The features of the coordination ne
Page 65: Catalysts within Dynamic Combinator
Page 69 and 70: Towards a Light Driven Proton Pump
Page 71 and 72: HPLC study of the complexation of c
Page 73 and 74: Novel fluorescent sensors based on
Page 75 and 76: SYNTHETIC TETRAAMIDES ANION RECEPTO
Page 77 and 78: Development of Vesicle-based Chemos
Page 79 and 80: Catechol derivatives of thiacalix[4
Page 81 and 82: Victor Pavlovsky Ukraine, Odessa "S
Page 83 and 84: Synthesis of disubstituted by merca
Page 85 and 86: SUPRAMOLECULAR POLYMERS OF OLIGO-DY
Page 87 and 88: Investigation of self-assembling ab
Page 89 and 90: Conjugates of thiacalixarenes and c
Page 91 and 92: Chromogenic Anion Receptors Based o
Page 93 and 94: Family Name First Name Title Positi
Page 95: Universität Regensburg - direction
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