International Summer School PROGRAM - Laboratoire d'Infochimie ...

FRET based distance measurements on vesicle surfaces - Towards the
dynamic recognition of multivalent target molecules
Stefan Balk a , Benjamin Gruber a , Tobias Lang a , Burkhard König a, *
a
University of Regensburg, Universitätsstraße 31, 93053 Regensburg
e-mail: burkhard.koenig@chemie.uni-regensburg.de
Mimicking recognition processes at natural cell membranes we recently reported synthetic vesicles
with multi-receptor surfaces as chemosensors for small biomolecules. [1] Based on these results we
want to develop a simple model system for the dynamic recognition of multivalent target molecules by
multiple membrane-embedded receptors equipped with FRET labels.
Figure 1. A multivalent biomolecule with two different ligands binding to two fluorescent labeled
receptor molecules, embedded in a membrane and detected via FRET.
The influence of analyte binding towards the FRET distances was investigated by emission titrations.
The investigation of these dynamic interactions is part of an approach towards imprinted vesicles with
ordered surfaces that perfectly match a templating target molecule.
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[1] B. Gruber, S. Stadlbauer, A. Späth, S. Weiss, M. Kalinina, B. König, Ang. Chem. Int. Ed. 2010, 49,
7125.