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THE UNIVERSITY OF CALGARY Eric Snively A ... - Ohio University

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Photoshop 3 for Macintosh, to ensure that the same hue was applied to each<br />

respective metatarsal cross section.<br />

The results were 57 images, saved as GIF format, with metatarsal cross<br />

sectional outlines filled with color. MT II, MT III, and MT IV were selected out of<br />

each image, and pasted into respective files. The new files had the same pixel<br />

dimensions as the originals, and the respective metatarsal images remained in<br />

the same position as in the original image. This meant the cross sections of the<br />

metatarsals would remain in the same relative position when the images were<br />

stacked for 3D reconstruction, and would maintain the same spatial relationships<br />

as the original bones. Images of individual rnetatarsal cross sections were also<br />

saved in GIF format.<br />

Sets of individual images for each metatarsat were then concatenated into<br />

three large files, containing data for al1 image slices of each respective<br />

metatarsal. These were saved as raw data fomat files, with names containing as<br />

much information as possible, to facilitate input for subsequent processing. For<br />

instance, the file containing red-filled outlines of the second metatarsal was<br />

called red-512x512x57.raw2, signtfying that there were 57 images each<br />

measuring 51 2 pixels square.<br />

3. Slice spacing calculations and curve detection<br />

Once these collective image files were created, they required preparation for<br />

curve detedion and stacking software. A program written by Dr. Doug Phillips,<br />

volume-to-nuages, performed the necessary calculations and format

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