3+ 4/2002 - Společnost pro pojivové tkáně

THE ORTHOSIS SPINOMED IMPROVES
POSTURE, TRUNK MUSCLE STRENGTH
AND QUALITY OF LIFE IN WOMEN
WITH SPINAL OSTEOPOROSIS
B. Begerow, M. Pfeiffer and H.W. Minne
Institut of Clinical Ostelogy, Bad Pyrmont, Germany
Thoracolumbar braces are widely used
in the care of patients with vertebral fractures
due to osteoporosis.Their usefulness,
however, has never been tested under standardized
conditions.To our knowledge, this
is first randomized, controlled, prospective,
cross over study to determine the efficacy
of an orthosis in the treatment of spinal
osteoporosis.
Spinomed improved posture, trunk
muscle strength, lung function, body sway,
and quality of life in postmenopausal
women with osteoporosis and may therefore
play an integral part in the rehabilitation
process of osteoporotic patients. The
efficacy of thoracolumbar braces needs to
the further investigated in prospective, randomized
and controlled clinical trials.
CBFA 1 EXPRESSION AND ITS
RELATIONSHIP TO THREE DIMEN-
SIONAL TISSUE STRUCTURE IN
HUMAN CHONDROCYTES IN VITRO
J. Rose, M. Schaller, M. Bayer et al.
Institut fuer Pathologie, Justus Liebig Universitaet
Giessen
CBFA 1 is a gene enconding a transcription
factor of the runt superfamily and one
of the earliest markers of osteoblastic differentiation.
Recent studies have shown as
well its importance for the maturation of
chondrocytes as well as for enchondral ossification
in CBFA 1 deficient mice. Obviously,
there is an additional role of CBFA 1 in
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the transdifferentiation of chondrocytes
into bone cells. However, regulation of
CBFA 1 gene expression is not yet understood.We
tested the hypothesis,if CBFA 1 is
present in human articular chondrocytes
and if a regulation of its expression can be
achieved from two to three dimensional in
vitro conditions.
The imunohistochemical stainings
revealed differentited chondrocytes with
a densely stained expression of collagen
type II. In all human articular chondrocytes
examined a CBFA 1 expression could be
demonstrated. Under monolayer in vitro
conditions, an increasing CBFA 1 mRNA
expression was detectable during subsequent
passages. A significant increase up to
200 % of passage 1 could be observed.
Using the alginate based bead system and
thus providing a three dimensional tissue
structure in vitro, the CBFA 1 level was only
maintained or even decreased.
This study demonstrates a regulation of
CBFA 1 expression dependent on a two or
three dimensional tissue structure in
human chondrocytes in vitro.This observations
suggest that for establishing and maitenance
of a stable cell differentation the tissue
architecture might be of major
importance. The underlying mechanisms
needs to be elucidaded yet.
MUDr. Miloslav Kuklík, CSc.
Ambulantní centrum pro vady pohybového aparátu
– genetická ambulance
Olšanská 7, 130 00 Praha 3