Colletotrichum: complex species or species ... - CBS - KNAW

Damm et al.
Fig. 29. Colletotrichum simmondsii (A–K, R-S from strain CBS 294.67. L–Q from ex-holotype strain CBS 122122). A–B. Conidiomata. C. Seta. D–K. Conidiophores. L–Q.
Appressoria. R–S. Conidia. A, C–F, R. from Anthriscus stem. B, G–Q, S. from SNA. A–B. DM, C–S. DIC, Scale bars: A = 200 µm, B = 100 µm, D = 10 µm. Scale bar of D
applies to C–S.
strain CBS 294.67, medium brown, smooth-walled, 0–1-septate,
20–40 µm long, base 2–3 µm diam, cylindrical, tip ± acute.
Conidiophores hyaline, smooth-walled, septate, branched, to 65
µm long. Conidiogenous cells hyaline, smooth-walled, cylindrical,
thred-like, 19–30 × 1 µm, opening 0.5 µm diam, collarette < 0.5
µm long, periclinal thickening not observed, conidiogenous cells
of other strains differ, e.g. conidiophores of CBS 294.67 are
cylindrical to slightly inflated and usually wider than the ex-type
strain, measuring 5–18 × 2.5–4.5 µm, opening 1–1.5 µm diam,
collarette 0.5–1 µm long, periclinal thickening visible. Conidia
hyaline, smooth-walled, aseptate, straight, cylindrical to fusiform
with both ends acute or one end round and one end acute, (6–)7–
10(–12.5) × (2–)2.5–3.5(–4.5) µm, mean ± SD = 8.4 ± 1.5 × 3.0
± 0.5 µm, L/W ratio = 2.8, conidia of other strains differ in shape
and size from the ex-type strain, e.g. conidia of CBS 294.67 are
cylindrical to fusiform with both ends acute and (11–)12–14.5(–
15.5) × (3–)4–4.5(–5) µm, mean ± SD = 13.3 ± 1.2 × 4.1 ± 0.4
µm, L/W ratio = 3.2.
Culture characteristics: Colonies on SNA flat with entire margin,
hyaline to to pale isabelline, on filter paper and on Anthriscus stem
partly covered with short white to pale grey felty aerial mycelium,
reverse of filter paper white to olivaceous grey, growth 10–16
mm in 7 d (16–26 mm in 10 d), other strains differ from the type
strain by growing faster, e.g. CBS 294.67 grows 20.5–31 mm in
7 d (34–40 mm in 10 d). Colonies on OA flat with entire margin;
surface covered with felty white aerial mycelium, becoming pale
olivaceous grey towards the centre, margin white or rosy buff,
reverse dark olivaceous grey or salmon and purplish to iron-grey
towards the margin, growth 10–16 mm in 7 d (14–24 mm in 10 d),
other strains differ from the type strain by growing faster, e.g. CBS
294.67 grows 21–27 mm in 7 d (32.5–40 mm in 10 d). Conidia in
mass not observed in the ex-type strain, those of other strains are
salmon-orange.
Material examined: Australia, Queensland, Yandina, from fruit anthracnose of of
Carica papaya, May 1987, L.M. Coates, culture ex-holotype CBS 122122 = BRIP
28519 = BCC 28680 = HKUCC 10928 = ICMP 17298 = KACC 43258; Queensland,
Brisbane, Ormiston, from fruit rot of Carica papaya, 1959, J.H. Simmonds, culture
ex-topotype of C. acutatum CBS 294.67 = BRIP 11084; Queensland, Brisbane,
Nambour, from fruit rot of Fragaria × ananassa, 30 Mar. 1965, J.H. Simmonds,
(according to BRIP database: K.G. Pegg), culture CBS 295.67 = BRIP 11086;
Western Australia, Wanneroo, from rotting fruit of Fragaria × ananassa, collection
date and collector unknown (deposited in IMI in 1992 by R.M. Floyd, Western
Australia Department of Agriculture, Australia, No. WA 2768), culture IMI 354381 =
CPC 18923. USA, Hawaii, from Protea cynaroides, 8 Dec. 1998, P.W. Crous & M.E.
Palm, culture CBS 114494 = STE-U 2964 = STE-U 2088.
Notes: Colletotrichum simmondsii was described by Shivas &
Tan (2009) to accommodate strains of the C. acutatum aggregate
assigned to group A2 by Sreenivasaprasad & Talhinhas (2005). The
type of C. simmondsii was erroneously designated as an epitype
of C. acutatum (i.e. s.str.) by Than et al. (2008b), before Shivas
& Tan (2009) recognised that the two taxa are not conspecific. In
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