Thermo Scientific Multidrop® 384 - Lab Equipment, Industrial ...

7.5 How to recalibrate the dispensing cassette
Chapter 7: Maintenance
The dispensing cassette has been calibrated by the manufacturer with deionized distilled water at
22°C ± 2°C. It is recommended you recalibrate the cassette after dispensing about 1000 96-well
plates or after every three months. It is also recommended you recalibrate the dispensing cassette if
you have forgotten the cassette in the Multidrop 384 under tension for prolonged periods, for example,
a weekend or longer.
Two different calibration methods are described below, that is, volumetric calibration and gravimetric
calibration.
7.5.1 Volumetric calibration
For volumetric calibration, use the following steps:
1. Remove the priming vessel and microplate.
2. Install the dispensing cassette into the Multidrop 384 if it is not already installed. Place the
dispensing cassette into the rest position (Fig. 6.18). Refer to section 6.3 How to insert the priming
vessel and the dispensing cassette.
3. Install the tube of the calibration device into the tip of the channel to be recalibrated (Fig. 7.5), for
example, A.
Fig. 7.5 Placing the tube onto the tip of channel A
4. Insert the dispensing cassette into the working position so that the lower and upper parts of the
dispensing cassette are inserted into their slots (Fig. 6.5).
5. Pull the rotor cover over the rotor.
6. Place the tube of channel A into the reservoir with water at room temperature (working
temperature).
Thermo Scientific Multidrop ® 384 User Manual; Rev. 3.4, Cat. no. 1507010 40