Untitled - The Future Ocean

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2.3.3 Research Platform P3: High-Throughput Molecular Bioscience TechnologiesCoordinators:Prof. Schreiber, StefanProf. Bosch, Thomas25.06.1962 03.09.1955Christian-Albrechts-Universität zu KielChristian-Albrechts-Universität zu KielInstitut für klinische MolekularbiologieZoologisches InstitutKlinik für Allgemeine Innere MedizinAm Botanischen Garten 1-9Schittenhelmstr. 1224118 Kiel24105 KielTel.: 0431-597 2350Tel.: 0431-880 4169Fax: 0431-597 1434Fax: 0431-880 4747Email: s.schreiber@mucosa.deEmail: tbosch@zoologie.uni-kiel.deFurther Proponents: U.-P. Hansen, C. Jung, M. Krawczak, G. Rimbach1. Summary / ZusammenfassungThe Center for Molecular Biosciences (ZMB) provides as platform P3 high-throughput molecularbiology and genetics technologies for the marine biology projects of the Cluster. The ZMB wasfounded in an interdisciplinary approach by three faculties of the Christian-Albrechts-University(Medicine, Mathematics and Natural Sciences, Agriculture and Nutritional Sciences) to concentrateautomated technologies within the life sciences. The five main areas of expertise within theplatform are (i) (ultra)high-throughput analyses of genetic diversity, (ii) systematic expressionprofiling, (iii) robot-assisted cell-based assays including automated genome-wide siRNAtransfection protocols, (iv) competitive proteome analysis techniques and (v) the availability of apopulation-representative biobank (PopGen). The state-of-the-art technologies form a tightlyconnected versatile network which will be able to adapt to the needs of the Cluster. Noveltechnologies for the generation of transgenic marine animal models, automated live microscopyand high-content image analysis will be developed together with the Cluster partners. Use of thestructures available at the ZMB for marine life sciences will provide a unique opportunity forcombining local excellence in content-driven research and cutting-edge technologies.Das Zentrum für Molekulare Biowissenschaften (ZMB) stellt als Plattform P3 des Clustersmolekulare Hochdurchsatztechnologien für die meeresbiologischen Projekte zur Verfügung. DasZMB wurde in einem interdisziplinären Ansatz von drei Fakultäten (Medizinische Fakultät,Mathematisch-Naturwissenschaftliche Fakultät und Agrar- und ErnährungswissenschaftlicheFakultät) gegründet. Die Plattform konzentriert fünf technologische Schwerpunkte in einerInstitution: (i) Hochdurchsatzanalyse von genetischer Diversität, (ii) systematische Expressionsprofilierung,(iii) roboter-unterstützte zellbasierte Assays ( z.B. genomweite RNAi Applikationen),101
(iv) Proteomanalysetechniken und (v) die populationsrepräsentative Proben- und Datensammlungder PopGen Biobank. Diese unterschiedlichen Technologien bilden ein flexibles Netzwerk, welchesschnell an den Bedarf der Clusterprojekte angepasst werden kann. Neue Technologien umfassendie Entwicklung von Modellen transgener Meerestiere und die automatisierte Live-Mikroskopie mitmultidimensionaler Bildanalyse. Der Einsatz der Technologien des ZMB bietet eine einzigartigeMöglichkeit die lokale Exzellenz der meeresbiologischen Inhaltsforschung und kompetitiveTechnologie auf einer neuen Ebene zu verknüpfen.2. State-of-the-artDeciphering the sequence of entire genomes is regarded as a major breakthrough in biomolecularresearch and has provided the basis for a deeper understanding of many organisms. Althoughmany definite answers are given, comprehensive genome analysis also raises numerous assailingquestions, many of which address the function of gene products in pathways and ultimatelyresulting in a phenotype. The challenge of high-content data generation and analysis for thepurpose of addressing genetic (sequence) variability in populations, the expression of genes, thedetection of the proteome and cell-based annotation of functions requires a broad range of cuttingedgetechnologies which can only be implemented in a central research facility. Key elements forsuccess are a high level of both standardization and versatility. Efficient networking with academicgroups as well as research groups from industry will be a prerequisite for the economical use ofresearch resources using high-throughput screening (HTS) technology in aquatic life sciences.3. Existing and Emerging TechnologiesCAU has established the Center for Molecular Biosciences (ZMB) to concentrate multifacetedcompetence in high-throughput genetics and genomics. The ZMB will move into a new state-ofthe-artbuilding centrally located on the campus by 2008. An additional level in the building will bededicated to small companies or small corporate research centers interested in close contact withZMB research activities. ZMB technology platforms have been formed through University activitiesin the National Genome Research Network (NGFN), the National Plant Genome Project (GABI),the National Genome Program on Livestock (FUGATO) and the BMBF Molecular Nutrition Group.The complete integration of the three founding University faculties (Medicine, Mathematics, NaturalSciences and Agriculture and Nutritional Sciences), is a unique example of the development ofnew university structures in one of its main areas of focus, the biosciences. The existing platformscomprise: (i) Multiple high-throughput technologies for the detection and assessment of DNAsequence and sequence variants. With large robotic facilities for sample handling and four capillarysequencers, the platform is part of the National Genotyping Platform of the NGFN and hasgenerated over 40 million genotypes and over 400,000 sequence traces within the last year. Thiscompetitive technology has aided the identification of complex disease genes for inflammatorybarrier disorders (Hampe et al. 2001, Stoll et al. 2004, Valentonyte et al. 2005) or neurobehavioraltraits (Spanagel et al. 2005). (ii) A systematic platform for the assessment of gene expression usesAffymetrix® microarray technology. Custom-made solutions can include specific arrays for102
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Contents1 General Information about
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1 General Information about the Clu
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1.2 Research Program1.2.1 Summary/Z
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1.2.2.2 ObjectivesThe Future Ocean
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will address the emerging new resea
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Topics Objectives DisciplinesA1 Exa
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development of these new initiative
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Project Objective IndustryPartnersO
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Continued excellence in the field o
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The establishment of several new po
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1.4.1 Integrated School of Ocean Sc
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ensure that emerging innovations wi
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Institute / DisciplineAcademic Leve
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their orientation according to the
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1.7.2 Structural Evolution and Qual
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30- Notes -
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organisms to elevated CO 2 and decr
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ist wahrscheinlich stärker als wä
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out by the proponents and will esta
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Rückkopplungsschleife weitere Erw
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enthic biota of gas release or the
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Synthese, aufbauend auf einer Kombi
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(4) a determination of the impact o
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Einflüsse. Um deren Auswirkungen b
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on longer time scales. The latter t
Page 57 and 58: werden kann. Allerdings sind die ch
Page 59 and 60: the expertise which already exists
Page 61 and 62: 2. State-of-the-artThe changing com
Page 63 and 64: • Field and laboratory investigat
Page 65 and 66: Erwärmung der Ozeane kann zur Frei
Page 67 and 68: Valuing the Ocean: Research focus a
Page 69 and 70: submarine earthquakes, slumps and s
Page 71 and 72: Fischerei ist eine der wichtigsten
Page 73 and 74: ecosystems. In particular, the foll
Page 75 and 76: Hochdurchsatztechnologien die Evolu
Page 77 and 78: microbial diversity on their barrie
Page 79 and 80: transient three-dimensional fluid f
Page 81 and 82: modeling, which describe chemical a
Page 83 and 84: Erdbeben, submarine Hangrutschungen
Page 85 and 86: Simons 2003) to investigate shallow
Page 87 and 88: damit zusammenhängender Meeresspie
Page 89 and 90: B5(1) Sea-Level Rise and Physical-M
Page 91 and 92: Pickrill RA, Todd BJ (2003) The mul
Page 93 and 94: Onate E, Piazzese J (2005) Decision
Page 95 and 96: echtlichen und ökonomischen Rahmen
Page 97 and 98: marine resources, such as energy ex
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Page 101 and 102: 2. State-of-the-artThe variety of n
Page 103 and 104: and will benefit from expertise in
Page 105 and 106: Die Plattform 2 stellt die analytis
Page 107: 4. New Cluster TechnologiesIn order
Page 111 and 112: 4. New Cluster TechnologiesA specia
Page 113 and 114: Illustration of existing/emerging a
Page 115 and 116: physical parameters (e.g. lowered a
Page 117 and 118: etabliert, das insbesondere auf wis
Page 119 and 120: 2.4.3.2 Central Funds for Transfer
Page 121 and 122: International School of Ocean Scien
Page 123 and 124: Description Year of purchase Amount
Page 125 and 126: 3.2 Auxiliary Support3.2.1 Total Fu
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Page 129 and 130: Suess E, Torres ME, Bohrmann G, Col
Page 131 and 132: Schreiber, StefanVisbeck, MartinSri
Page 133 and 134: 4.3 Third-Party FundingNo. FundingB
Page 135 and 136: Leibniz Institute of Marine Science
Page 137 and 138: These JRG’s will augment the expe
Page 139 and 140: The Cluster is embedded in the “K
Page 141 and 142: A14- Notes -
Page 143 and 144: 4.6 Curricula Vitae and Lists of Pu
Page 145 and 146: Curriculum of ResearchPersonal Data
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Curriculum of ResearchPersonal Data
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A82- Notes -
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DEKLIMGerman Climate Research Progr
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ITQ’sISAISOSJRGKCMSKitzLALIFLIMSL
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WTOWTSHXAFSXRDZMBWorld Trade Organi
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Prof. Dr. Boris Culik • Maritimes
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GMT-Geschäf*sstelleWe"*w{eltJ"*n $
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f,rylheonRaytheon Anschütz GmbHPos
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