team II: <str<strong>on</strong>g>the</str<strong>on</strong>g> search <str<strong>on</strong>g>for</str<strong>on</strong>g> chemical <str<strong>on</strong>g>in</str<strong>on</strong>g>dicators of life/II.5 RECOMMENDED PAYLOAD AND TECHNOLOGY RESEARCH PROGRAMME Tak<str<strong>on</strong>g>in</str<strong>on</strong>g>g <str<strong>on</strong>g>in</str<strong>on</strong>g>to account <str<strong>on</strong>g>the</str<strong>on</strong>g> mass, power and volume restricti<strong>on</strong>s, <str<strong>on</strong>g>the</str<strong>on</strong>g> follow<str<strong>on</strong>g>in</str<strong>on</strong>g>g <str<strong>on</strong>g>in</str<strong>on</strong>g>strumentati<strong>on</strong> appears to be essential <str<strong>on</strong>g>for</str<strong>on</strong>g> reach<str<strong>on</strong>g>in</str<strong>on</strong>g>g <str<strong>on</strong>g>the</str<strong>on</strong>g> exobiological objectives of <str<strong>on</strong>g>the</str<strong>on</strong>g> package as described earlier <str<strong>on</strong>g>in</str<strong>on</strong>g> this document. FIRST PRIORITY Microscope: <str<strong>on</strong>g>for</str<strong>on</strong>g> general exam<str<strong>on</strong>g>in</str<strong>on</strong>g>ati<strong>on</strong> of <str<strong>on</strong>g>the</str<strong>on</strong>g> samples – resoluti<strong>on</strong>: 3 µm – mass: 250 g (100 g, with <str<strong>on</strong>g>the</str<strong>on</strong>g> close-up imager but with 50 µm resoluti<strong>on</strong>) – heritage: Beagle-2 Raman spectroscopy: molecular analysis of m<str<strong>on</strong>g>in</str<strong>on</strong>g>erals and organics (with IR) – must <str<strong>on</strong>g>in</str<strong>on</strong>g>clude near IR excitati<strong>on</strong> <str<strong>on</strong>g>for</str<strong>on</strong>g> biological applicati<strong>on</strong>s, as well as geochemical – spectral range: 200-3500 cm -1 – resoluti<strong>on</strong>: 8 cm -1 – projected mass: 1 kg – heritage: <strong>Mars</strong> Surveyor 2001 APX Spectrometer: elemental analysis, detecti<strong>on</strong> limit a few 0.1% – mass: 570 g – power: 340 mW – data output: 16 Kbytes per sample analysis – heritage: <strong>Mars</strong> Pathf<str<strong>on</strong>g>in</str<strong>on</strong>g>der, <strong>Mars</strong> Surveyor 2001, Rosetta Mössbauer Spectrometer: quantitative analysis of Fe – mass: 500 g – power: 1.5 W – radioactive source of about 300 mCi – heritage: <strong>Mars</strong> Surveyor 2001 A<str<strong>on</strong>g>the</str<strong>on</strong>g>na missi<strong>on</strong> (MIMOS II <str<strong>on</strong>g>in</str<strong>on</strong>g>strument) PYR-GC-MS system: isotopic, elemental, organic and <str<strong>on</strong>g>in</str<strong>on</strong>g>organic molecular compositi<strong>on</strong>, and chirality measurement – PYR: <str<strong>on</strong>g>in</str<strong>on</strong>g>cludes several ovens (<str<strong>on</strong>g>for</str<strong>on</strong>g> pyrolysis, combusti<strong>on</strong> and chemical trans<str<strong>on</strong>g>for</str<strong>on</strong>g>mati<strong>on</strong> of <str<strong>on</strong>g>the</str<strong>on</strong>g> samples) – GC: a m<str<strong>on</strong>g>in</str<strong>on</strong>g>imum of four columns, <str<strong>on</strong>g>in</str<strong>on</strong>g> parallel, likely to be capillary open columns <str<strong>on</strong>g>for</str<strong>on</strong>g> separati<strong>on</strong>, respectively of: permanent gases and very low molecular weight organics volatile higher molecular weight organics of small polarity volatile higher molecular weight organics of high polarity column <str<strong>on</strong>g>for</str<strong>on</strong>g> enantiomer separati<strong>on</strong> (chiral or, if derivatisati<strong>on</strong> with chiral reactant, n<strong>on</strong>-chiral column) heritage: COSAC additi<strong>on</strong>al detectors (nano-TCDs, chiral detectors) <str<strong>on</strong>g>for</str<strong>on</strong>g> each GC column – MS: i<strong>on</strong> trap (heritage: MODULUS) or magnetic (Beagle-2) or Quadrupole (heritage: CHARGE). In all cases, a pump<str<strong>on</strong>g>in</str<strong>on</strong>g>g device (such as a m<str<strong>on</strong>g>in</str<strong>on</strong>g>iaturised turbo molecular pump) will have to be developed. total mass: 5.5 kg power: 10-20 W (to be c<strong>on</strong>firmed) H 2 O 2 and o<str<strong>on</strong>g>the</str<strong>on</strong>g>r oxidant-dedicated sensors (still to be fully studied and developed): – expected mass: 100 g (to be c<strong>on</strong>firmed) SECOND PRIORITY Infrared spectroscopy: molecular analysis of m<str<strong>on</strong>g>in</str<strong>on</strong>g>erals and organics (with Raman) – wavelength range: 0.8-10 µm – spectral resoluti<strong>on</strong>: >100 (λ/∆λ) – spatial resoluti<strong>on</strong>: 200 µm – expected mass:
SP-1231 154 References achiral column (i.e. <str<strong>on</strong>g>for</str<strong>on</strong>g> compounds with more than <strong>on</strong>e chiral centre). Let us also menti<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> recent development of piezoelectric and optical gas sensors that ‘are capable of recognis<str<strong>on</strong>g>in</str<strong>on</strong>g>g different enantiomers and of qualitatively m<strong>on</strong>itor<str<strong>on</strong>g>in</str<strong>on</strong>g>g <str<strong>on</strong>g>the</str<strong>on</strong>g> enantiomeric compositi<strong>on</strong> of am<str<strong>on</strong>g>in</str<strong>on</strong>g>oacid derivatives and lactates <str<strong>on</strong>g>in</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> gas phase’ (Bodenhoefer et al., 1997). Such a technique could be applied to a GC-MS system <str<strong>on</strong>g>in</str<strong>on</strong>g>clud<str<strong>on</strong>g>in</str<strong>on</strong>g>g a solid- or gas-state derivatisati<strong>on</strong> process <str<strong>on</strong>g>for</str<strong>on</strong>g> analys<str<strong>on</strong>g>in</str<strong>on</strong>g>g am<str<strong>on</strong>g>in</str<strong>on</strong>g>o acids after derivatisati<strong>on</strong> <str<strong>on</strong>g>in</str<strong>on</strong>g>to volatile compounds. Afflalaye, A., Sabah, A., Sternberg, R., Raul<str<strong>on</strong>g>in</str<strong>on</strong>g>, F. & Vidal-Madjar, C. (1996). Gas chromatography of Titan’s atmosphere. VI: Analysis of low molecular weight hydrocarb<strong>on</strong>s and nitriles with cyanopropylphenyl: dimethylpolysiloxane. J. Chromatogr. 746, 63-69. Afflalaye, A., Sternberg, R., Coscia, D., Raul<str<strong>on</strong>g>in</str<strong>on</strong>g>, F. & Vidal-Madjar, C. (1997). Gas chromatography of Titan’s atmosphere. VIII: Analysis of permanent gases with carb<strong>on</strong> molecular sieve packed capillary columns. J. Chromatogr. A76, 195-203. Bodenhöfer, K., Hierlemann, A., Seemann, J., Gauglitz, G., Koppenhoefer, B & Göpel, W. (1997). Chiral discrim<str<strong>on</strong>g>in</str<strong>on</strong>g>ati<strong>on</strong> us<str<strong>on</strong>g>in</str<strong>on</strong>g>g piezoelectric and opticalgas sensors. Nature 387, 577-580. Chambers, L.A. (1982). Sulfur isotope study of a modern <str<strong>on</strong>g>in</str<strong>on</strong>g>tertidal envir<strong>on</strong>ment, and <str<strong>on</strong>g>the</str<strong>on</strong>g> <str<strong>on</strong>g>in</str<strong>on</strong>g>terpretati<strong>on</strong> of ancient sulfides. Geochim. Cosmochim. Acta 46, 721-728. Edwards, H.G.M., Farwell, D.W., Grady, M.M., Wynn-Williams, D. & Wright, I.P. (1999). Comparative Raman microscopy of a martian meteorite and Antarctic lithic analogs. Planet Space Sci. 47, 353-362. Habicht, K.S. & Canfield, D.E. (1997). Sulfur isotope fracti<strong>on</strong>ati<strong>on</strong> dur<str<strong>on</strong>g>in</str<strong>on</strong>g>g bacterial sulfate reducti<strong>on</strong> <str<strong>on</strong>g>in</str<strong>on</strong>g> organic-rich sediments. Geochim. Cosmochim. Acta 61, 5351- 5361. Imbus, S.W. & McKirdy, D.M. (1993). Organic geochemistry of Precambrian sedimentary rocks. In Organic Geochemistry (Eds. M.H. Engel & S.A. Macko), Plenum, pp657-684. Irw<str<strong>on</strong>g>in</str<strong>on</strong>g>, W.J. (1982). Analytical pyrolysis – a comprehensive guide. Chromatographic Science Series, 22, Marcel Dekker, New York, USA. Israel, G., Niemann, H., Raul<str<strong>on</strong>g>in</str<strong>on</strong>g>, F., Riedler, W., Atreya, S., Bauer, S., Cabane, M., Chassefiere, E., Hauchecorene, A., Owen, T., Sable, C., Samuels<strong>on</strong>, R., Torre, J.P., Vidal-Madjar, C., Brun, J.F., Coscia, D., Ly, R., T<str<strong>on</strong>g>in</str<strong>on</strong>g>tignac, M., Steller, M., Gelas, C., C<strong>on</strong>de, E. & Millan, P. (1997). <str<strong>on</strong>g>The</str<strong>on</strong>g> Aerosol Collector Pyrolyser (ACP) experiment <str<strong>on</strong>g>for</str<strong>on</strong>g> Huygens. <strong>ESA</strong> SP-1177, 59-84. Kaplan, I.R. & Rittenberg, S.C. (1964). Microbiological fracti<strong>on</strong>ati<strong>on</strong> of sulfur isotopes. J. Gen. Microbiol. 34, 195-212. Kl<str<strong>on</strong>g>in</str<strong>on</strong>g>gelhöfer, G., Fegley Jr., B., Morris, R.V., Kankeleit, E., Held, P., Evlanov, E. & Priloutski, O. (1996). M<str<strong>on</strong>g>in</str<strong>on</strong>g>eralogical analysis of Martian soil and rock by a m<str<strong>on</strong>g>in</str<strong>on</strong>g>iaturised backscatter<str<strong>on</strong>g>in</str<strong>on</strong>g>g Mössbauer spectrometer. Planet. Space Sci. 44, 1277-1288. Lambert, I.B. & D<strong>on</strong>nelly, T.H. (1990). <str<strong>on</strong>g>The</str<strong>on</strong>g> paleoenvir<strong>on</strong>mental significance of trends <str<strong>on</strong>g>in</str<strong>on</strong>g> sulphur isotope compositi<strong>on</strong>s <str<strong>on</strong>g>in</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> Precambrian: a critical review. In Stable Isotopes and Fluid Processes <str<strong>on</strong>g>in</str<strong>on</strong>g> M<str<strong>on</strong>g>in</str<strong>on</strong>g>eralisati<strong>on</strong> (Eds. H.K. Herbert & S.E. Ho), Univ. Western Austr. Spec. Publ. 23, 260-268. de Leeuw, J.W., & Largeau, C. (1993). A review of macromolecular organic compounds that comprise liv<str<strong>on</strong>g>in</str<strong>on</strong>g>g organisms and <str<strong>on</strong>g>the</str<strong>on</strong>g>ir role <str<strong>on</strong>g>in</str<strong>on</strong>g> kerogen, coal and petroleum <str<strong>on</strong>g>for</str<strong>on</strong>g>mati<strong>on</strong>. In Organic Geochemistry (Eds. M.H. Engel & S.A. Macko), Plenum, pp23-72. MacDermott, A.J. et al. (1996). Homochirality as <str<strong>on</strong>g>the</str<strong>on</strong>g> signature of life: <str<strong>on</strong>g>the</str<strong>on</strong>g> SETH Cigar. Planet. Space Sci. 44, 1441-1446. Meuzelaar, H.L.C., Haverkamp, J. & Hileman, F.D. (1982). Pyrolysis mass spectrometry of recent and fossil biomaterials – compendium and atlas. Techniques and Instrumentati<strong>on</strong> <str<strong>on</strong>g>in</str<strong>on</strong>g> Analytical Chemistry, 3, Elsevier, Amsterdam, <str<strong>on</strong>g>The</str<strong>on</strong>g> Ne<str<strong>on</strong>g>the</str<strong>on</strong>g>rlands. Morris, R.V., Squyres, S.W., Bell III, J.F., Christensen, P.H., Ec<strong>on</strong>omu, T., Kl<str<strong>on</strong>g>in</str<strong>on</strong>g>g-
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SP-1231 Exobiology
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Cover Fossil coccoid bacteria, 1 µ
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4 I.5 Potential Non-Martian Sites <
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6 6.2 Imaging of F
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The Exobio
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pyrolysis, or similar techniques, f
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Ignasi Casanova, Institute
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I AN EXOBIOLOGICAL VIEW OF THE SOLA
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SP-1231 18 surface pressure of CO 2
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SP-1231 20 10 bar befor</st
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SP-1231 22 kilometres in</s
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SP-1231 24 Laboratory Investigation
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I.3 Limits of Life
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temperatures of 95-106ºC. This sug
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(mainly found <str
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cannot exclude fin
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Responses to Cosmic Radiation <stro
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acid to identify the</stron
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Horneck, G. (1993). Responses of Ba
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SP-1231 Fig. I.4.2.2/1. Size distri
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SP-1231 Fig. I.4.2.2/4. Evaporite w
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SP-1231 Fig. I.4.2.3/1A (left). Net
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SP-1231 48 A detailed chemical anal
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SP-1231 Fig. I.4.3.1.1/1. Scheme of
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SP-1231 Fig. I.4.3.1.2/1. A: Compar
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SP-1231 54 I.4.3.2.1 Sedimentary Or
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SP-1231 56 The iso
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SP-1231 Fig. I.4.3.2.3/1. Cholester
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SP-1231 60 References regard to non
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SP-1231 62 Klein,
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SP-1231 64 ities in</strong
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SP-1231 Fig. I.5.1.1/1. A 34×42 km
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SP-1231 Fig. I.5.2.1/1. Titan’s a
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SP-1231 Fig. I.5.2.2/2. Modelled Ti
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SP-1231 72 Galileo: images availabl
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SP-1231 74 TABLE I.6.2/1 EVIDENCE O
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SP-1231 I.6.3 What to Searc
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SP-1231 78 I.7.3 Organic Chemistry
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II.1 Introduction The</stro
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II.2 The Planet Ma
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cover the range 4.
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The depletion of c
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valley networks (Fig. II.2.5/2) are
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plate tectonic evidence has been ob
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Table II.2.7.4/1. Potential Mars <s
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II.3 The Martian M
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principal atmosphe
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Lines of evidence
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