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No. 43 (PH 9)
The comparison of the notations for quantitative evaluation of adhesive molecules’
expression on CD34 positive cells
Nobuo Masauzi 12 , Junji Tanaka 2 , Masaharu Kasai 3 , Masahiro Ogasawara 3 , Minami Doi 4 , Naoki
Kobayashi 3 , Yoshio Kiyama 3 , Minami Doi 4 , Sayaka Fukui 4 , Nao Fujimoto 4 , Misaki Yamada 4 ,
Keiko Miwa, 1 , Masanobu Kobayashi 5 ,Masahiro Imamura 3
1: Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University.
Sapporo, 2: Department of Hematology, Hokkaido University Graduate School of Medicine, Sapporo, 3:
Department of Hematology, Sapporo Hokuyu Hospital. Sapporo, 4: Department of Health Science,
School of Medicine, Hokkaido University, Sapporo, 5: Department of Nursing, Health Science
University of Hokkaido, Ishikari, Japan.
There are various quantitative notations for molecule expression on cells, such as the percentage of
positive expressing cells (PPC) and the mean fluorescence intensity (MFI). As to MFI, some evaluate
only MFI-PF among cells in positive fraction (PF), which express blighter fluorescence than that of
iso-type control anti-bodies, the others do MFI-PF&NF among cells in PF and negative fraction
(NF).We had published that the MFI-PF&NF of CD11a and CD11b on CD34 positive cells(CD34+) in
peripheral blood (PB) were inversely correlated to the yields of total collectedCD34+ before
administration of granulocyte-colony stimulating factor (G-CSF). Although some authors have
reported similar analysis, they represented the expressions of these adhesion molecules by PPC or
MFI-PF. Thus, the direct comparison of these results was impossible. We have analyzed the
significance of the change of PPC, MFI-PF and MFI-NF of CD11a and CD11b, and the correlations of
the yield of PB graft (YPBG). No significant changes ware indicated with 2-way ANOVA in PPC
neither by the number of days administrating G-CSF (Days), nor the YPBG, while there were
significant changes in MFI-PF&NF. The same test indicated not only significant change of MFI-PF of
CD11a by Days, but also that of MFI-NF of CD11a by YPBG. The significant correlation between the
PPC and the YPBG was not shown. MFI-PFs of CD11a and CD11b on day -1 indicated significant
inverse correlations with the YPBG. MFI-NFs of CD11a and CD11b indicated no significant
correlations with the YPBG The value of the lower fluorescence, which derived from non-specific
binding of anti-bodies, was commonly recognized as meaningless. The presenting results suggested that
the value of the lower fluorescence area had some important mean in a certain situation.
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