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No. 29 (PI 3)
Development of ABO and Lewis typing by enzyme-linked immunosorbent assay for
disaster
Terutaka Sagawa and Mariko Okada
Division of Biomedical Sciences, Institute of Medical Technology, Ehime Prefectural University Of
Health Sciences, Japan
For ABO blood group typing, we need electrical power, the clean area for collecting venous blood and
substantial experience of technologists. We have little electrical power, clean areas and human resources
after a disaster, just like a giant earthquake or a giant tsunami, occurs. In this case, there is a need of the
method that does not require electrical power, the clean area and substantial experience.
We have developed ABO and Lewis blood group typing by enzyme-linked immunosorbent assay
( ELISA ) using Japanese saliva as antigen. Tween 20 was used to block and wash. This method could
give a result within 120 minutes from saliva collecting procedure.
All secretors (88% of the samples examined) reacted with anti-H Ab.
Nonsecretors reacted weakly with anti-H Ab or not. A-type and AB-type nonsecretors reacted weakly
with anti-A Ab. B-type and AB-type nonsecretors reacted weakly with anti-B polyclonal antibody but
not anti-B monoclonal antibody.
Secretors included Lea-Leb- (19% of secretors), Lea-Leb+ (28%) and Lea+Leb+ (53%). All
nonsecretors reacted with anti-Lea Ab not anti-Leb Ab.
Kudo et al. demonstrated that almost Japanese nonsecretors had sej/sej homozygosity. The sej/sej has
an elevated ABH antigen. No activity alleles are very rare in Japanese.
It was proved that the ELISA method reported here was useful for almost Japanese ABO and Lewis
blood group typing, and did not require electrical power and substantial experience. Further
developments are necessary for very rare Japanese complete nosecretors, and Rh typing and ABO
reverse typing.
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