Program / Abstract Book - KMU WWW3 Server for Education ...
Program / Abstract Book - KMU WWW3 Server for Education ...
Program / Abstract Book - KMU WWW3 Server for Education ...
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No. 27 (PI 1)<br />
Combination of Anti-HCV Assay to Improve Per<strong>for</strong>mance of Hepatitis C Screening<br />
Test in Prodia National Reference Laboratory, Indonesia<br />
Romimatul Fadhilah, Yenny Surjawan, Indriyanti R. Sukmawati<br />
Prodia Clinical Laboratory, Indonesia<br />
Anti-HCV assay <strong>for</strong> hepatitis C screening test has been widely used. Based on CDC guideline (2003),<br />
positive anti-HCV screening result has to be verified by immunoblotting assay or nucleic acid testing. In<br />
fact, not all laboratories in Indonesia have facilities to per<strong>for</strong>m this test. Prodia National Reference<br />
Laboratory received samples <strong>for</strong> anti-HCV screening test from its hundred branches. Samples sources<br />
were from vary population with varying prevalence of hepatitis C. In our experience, samples referred<br />
<strong>for</strong> anti-HCV assay from other laboratories quite often showed discordance results with ours. Previous<br />
evaluation of 4 automated analyzers <strong>for</strong> anti-HCV assay by Kim et al (2008) showed good sensitivity<br />
(100 %), but 3.7 % still showed false positive results. There<strong>for</strong>e an evaluation of routine anti-HCV<br />
screening test should be per<strong>for</strong>med. We evaluated 55 samples using 3 analyzers (Advia Centaur,<br />
Architect-i2000 and Axsym). Seventeen samples that showed discordant results among analyzers or had<br />
extreme low and high index or s/co were confirmed by immunoblotting assay using INNO-LIA TM HCV<br />
Score reagent (Innogenetics). The sensitivity of anti-HCV assay on those 3 analyzers was good (100 %).<br />
False positive results were found in 4 samples by Architect, 5 samples by Advia and 5 samples by<br />
Axsym. The assay showed a different result if a sample, especially sample with low s/co ratio or index,<br />
was examined with different analyzers. The false positive results were not found in the same samples<br />
and there were no specific pattern. There<strong>for</strong>e, we consider using a second anti-HCV assay as<br />
recommended by UK Health Protection Agency algorithm (2007) <strong>for</strong> results with low s/co ratio or index<br />
to reduce the possibility of false positive result.<br />
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