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No. 33 (PI 7)
Immune response to MSP2 antigen of plasmodium falciparum in mamuji, west
Sulawesi, Indonesia
Nurhayana Sennang 1 , Dianawaty Amiruddin 2 , Bahrani 2 , Sitti Wahyuni 2 , Syafruddin 2,4 , Irawan
Yusuf 3 , Stephen Rogerson 5
1 2 3
Clinical Pathology Department, Parasitology Department, Physiology Department, Faculty of
Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia; 4 Eijkman Institute, Jakarta,
Indonesia; 5 Department of Medicine, University of Melbourne, Post Office Royal Melbourne Hospital,
Melbourne, Victoria, Australia
Background: A malaria parasite antigen, merozoite surface protein 2 (MSP-2), is assumed to be
involved in initial attachment of merozoite to erythrocyte surface. MSP-2 may contribute to the
development of clinically protective immunity to malaria which is dependent upon cumulative exposure
to the many parasite variants circulating in the local population. The aim of this study is to determine
immune response to MSP-2 P.falciparum in individuals living in Mamuju, an epidemic area of malaria
in West Sulawesi, Indonesia. Methods: The cross sectional study was conducted using multi-stages
random sampling from 15 districts (123 villages) in Mamuju, West Sulawesi, Indonesia. Capillary blood
samples from 4406 participants were collected during August 2010. All positive samples and 5%
negative samples based on blood smear results were selected randomly for immunological investigation.
IgG antibodies to MSP-2 were analyzed by Enzyme Linked ImmunoSorbent Assay (ELISA) Results:
Of 209 samples based on blood smear results, there were 18 samples with P.falciparum parasitaemia, 10
samples with P.vivax parasitaemia and 181 negatives samples, Means of IgG antibody to MSP2 in
individuals who had positive P.falciparum blood smears was 37.54 units, higher than positive P.vivax
(7.48 units) and negative blood smears (2.61 units), there was a significant difference between the three
groups (95%CI: p=0.0001). Cut off point of IgG antibody to MSP2 was 4.57 units. Of 37 samples with
levels of IgG antibody to MSP2 more than cut off, 11 were from individuals with P.falciparum
parasitaemia, 3 from individuals with P.vivax, and 23 from uninfected people. Conclusion: Increased
level of IgG antibody to MSP2 was associated with infection with P.falciparum. The presence of IgG
antibody to MSP2 in individuals with P.vivax infection or negative blood smears may indicate
mix-infection of P.falciparum undetected by blood smear or previous infection with P.falciparum.
Keywords: immune response, MSP-2, Plasmodium falciparum
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