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No. 93 (PO 7)
TNF-β gene-polymorphism in population and clinical studies
B.Ankhchimeg 1,3 , B.Munkhbat 1,2 , B.Gansuvd 1,2 , G.Oyungerel 1 , T.Shimura 2 N.Munkhtuvshin 1
1
Central Scientific Research Laboratory, Nat’l Institute of Medicine, Mongolia
2
Department of Transplantation Immunology, Tokai University, Japan
3
Dept. Laboratory Medicine, General Hospital, Arkhangai, Mongolia
Objectives: Genes of TNF-α (cachectin) and TNF-β (lymphotoxin) are located between HLA-B and
HLA class III, and no allelic polymorphism has been determined in the region of TNF-α loci, where two
alleles TNF-β1 and TNF-β2 has been described, that can inherits in three forms and no gene
frequencies and disease associations yet been fully studied.
Materials and methods: Ncol enzyme based RFLP allelic frequency of TNF-β (lymphotoxin) gene
analysis was performed in 126 healthy Mongolians (85 Khoton , and 41 Khalkh ethnic groups) and 135
Japanese lung cancer patients studied in comparison to 165 normal healthy control subjects.
Results: Gene frequency of 5.5/5.5-kb allele revealed in 12.2% of Khalkh population, and this is much
more frequent in comparison to Khoton population, in whom it was revealed only in 3.5%. But TNF-β
10.5/10.5-kb allele was determined in 49 cases (57.4%) in Khoton population, that was very high in
frequencies in comparison to both Khalkh and Japanese healthy group (34.1% and 41.1%),
(p=0.013440). In the lung cancer patients, the TNF-β 10.5/10.5-kb allele was found at a low frequency,
38.5%, compared to 53.3% in normal controls (chi 2 = 7.51, P = 0.011, corrected P = 0.033, relative risk
= 0.77). In the relationship between the histologic types and the TNF beta 10.5/10.5-kb allele showed
low frequencies: 38.5% in adenocarcinoma, 38.2% in squamous cell carcinoma, and 27.8% in small cell
carcinoma, although no statistical difference was shown. In relation to the postoperative survival period,
the TNF beta 10.5/10.5-kb allele was associated with prolonged survival.
CONCLUSIONS: The TNF beta 10.5/10.5-kb allele may be associated with resistance to lung cancer
and with a better prognosis.
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