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No. 30 (PI 4)
Evaluation of Hepatrio multiplex kit for early and simultaneous detection of
hepatitis A, B and C
Chi Hyun Cho, M.D. 1 , Sang Kyung Jo, M.D. 2 , Young Joo Kwon, M.D. 2 , Chae Seung Lim, M.D. 1
and Yun Jung Cho, M.D. 1*
1
Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea
2
Department of Nephrology and Hypertension, College of Medicine, Korea University, Seoul, Korea
Introduction: In hemodialysis patients, early detection of hepatitis A, B and C is very important. While
serology test for detection of hepatitis A, B and C is used, it has limitation in that it does not catch the
early infection and co-infection of hepatitis A, B and C. Recently a multiplex PCR kit for the
simultaneous detection of hepatitis A, B and C, Hepatrio, was developed. We evaluated the multiplex kit
in hemodialysis patients in one Korea hospital and compared the result with serology.
Methods: HAV IgM, HBsAg, anti-HBs, anti-HBc and HCV Ab were analyzed on 186 samples of
routine check-up examinees in a hemodialysis care unit by chemiluminescence immunoassay. Nucleic
acid amplification test (NAT) was performed on the same samples by multiplex real-time polymerase
chain reaction (PCR) kit for simultaneous detection of HAV, HBV, and HCV (Magicplex Hepatrio;
Seegene, Seoul, South Korea).
Results: The positive rates of HAV, HBV, and HCV were 0, 5.9, and 0.6%, respectively. All samples
except two showed consistent results between serology and multiplex PCR. Occult HCV infection was
found in 1 (0.6%) of 173 HCV-Ab negative subjects. Meanwhile, a HBs-Ag positive sample was not
detected for HBV-DNA by multiplex PCR. There was no co-infection case among samples.
Conclusion: This study suggests the possibility that multiplex PCR replaces serology for screening of
hepatitis A, B and C. Further study using much more samples is needed for the clinical performance
evaluation of multiplex PCR test. Key word: Hepatitis A, hepatitis B, hepatitis C, hemodialysis,
serology, multiplex PCR kit
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