Program / Abstract Book - KMU WWW3 Server for Education ...
Program / Abstract Book - KMU WWW3 Server for Education ...
Program / Abstract Book - KMU WWW3 Server for Education ...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
No. 78 (PMB 4)<br />
Study of essential oil <strong>for</strong> anti-allergy effect in murine asthma model<br />
Tomoe Ueno Iio, Misako Shibakura, Michinori Aoe, Tomoko Hyoda, Mihoko Kohara, Naohisa<br />
Nakagawa, Takako Nakahara, Mikio Kataoka.<br />
Graduate School of Health Sciences, Okayama University., Japan<br />
Background: The essential oils are used <strong>for</strong> aromatherapy classified into alternative medicine.<br />
However, there are few evidences of the action mechanism of essential oil <strong>for</strong> the body. To elucidate<br />
the possibility of the prevention of an allergic disease by the aromatherapy, we investigated the effects<br />
of essential oil using murine asthma model.<br />
Methods: Female BALB/c mice of 8-9 weeks were divided into a control mice (A), an asthma mice (B),<br />
the essential oil (Lavandula angustifolia) treated-asthmatic mice (C) and (D). To make asthma mice, B,<br />
C, and D were sensitized by intraperitoneal injection of Ovalbumin (OVA) at day 0 and 14, and<br />
challenged via airways with OVA on Days 28, 29, 30. After 48 to 72 hours later of last OVA challenge,<br />
airway resistances were assessed. The cell numbers, cytokine levels in BAL fluid, and serum<br />
OVA-specific IgE levels were measured. The mucus-containing cells were counted in periodic<br />
acid-Schiff (PAS) stained sections. For C and D, 5 µl and 20 µl of essential oil were applied <strong>for</strong> 20<br />
min from day 14, 5days of week.<br />
Results: The airway resistance significantly increased in B, C and D. The total cell numbers were<br />
significantly reduced in C and D compared with B. The eosinophil numbers were significantly<br />
reduced in D compared with B. IL-13 levels in BAL fluid were significantly reduced in group D<br />
compared with group B. The serum IgE levels were increased in each group except <strong>for</strong> A. There<br />
were significant reductions of the PAS positive cells in C and D compared with B.<br />
Conclusion: Treatment with Lavender essential oil of asthma mice decreased the number of total cells<br />
and eosinophils, IL-13 levels in BALF, and bronchoalveolar PAS positive cells. These results are<br />
indicating that aromatherapy may have possibility to be used <strong>for</strong> prevention of allergic inflammation,<br />
such as asthma.<br />
- 131 -
Change language