Program / Abstract Book - KMU WWW3 Server for Education ...
Program / Abstract Book - KMU WWW3 Server for Education ...
Program / Abstract Book - KMU WWW3 Server for Education ...
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No. 32 (PI 6)<br />
Discrepancy between serologic and genotyping results of ‘Mi a ’ blood antigen and<br />
antiserum in Taiwan<br />
Chien-Feng Sun 1,2 , Tai-Di Chen 2 , Ding-Ping Chen 2<br />
1 Department of Anatomic Pathology, 2 Department of Laboratory Medicine, Linkou Medical Center,<br />
Chang Gung Memorial Hospital, Taoyuan, Taiwan<br />
Aim. Mi.Ⅲ (GP.Mur) is reported to have a mean frequency of 7.3% among Taiwanese. Since the<br />
specificities of Mi.III (GP.Mur) antisera are not further specified, such antisera and the cells identified<br />
by them have been applied a collective term as anti-‘Mi a ’ and ‘Mi a ’(+) cells in Taiwan. Anti-‘Mi a ’<br />
antibodies are the most common clinically important antibodies detected in Taiwan. Our aim of this<br />
study is to evaluate the efficiency of current practice of using ‘Mi a ’(+) screening cells in comparing to<br />
genotyping <strong>for</strong> Miltenberger series.<br />
Materials and Methods. Blood samples were obtained from randomly selected patients. Antisera<br />
containing anti-‘Mi a ’ specificity were collected routinely in our blood bank. Manual polybrene method<br />
without a supplementary antiglobulin phase was used <strong>for</strong> serologic test. The results of serologic test<br />
were compared with PCR-sequencing data. The study was approved by our Institutional Review Board.<br />
Results. The prevalence of Mi.Ⅲ(GP.Mur) is 6.4% (25/389). Among 1945 serologic tests <strong>for</strong> these<br />
389 samples, 210 tests showed a positive reaction (1+ or more). Among them, only 84 positive results<br />
belonged to PCR (+) samples. The sensitivity and specificity of our routine practice using ‘Mi a ’(+)<br />
screening cells to detect anti-‘Mi a ’ antibodies are thus estimated to 67.2% (84/125) and 93.1%<br />
(1694/1820), respectively. The positive predictive value is 40% (84/210).<br />
Conclusions. Our results showed that the Mi.III (GP.Mur) has a prevalence of 6.4% in Taiwan, and no<br />
GP.Hut, GP.Hop, GP.Bun, or GP.HF was detected. However, our study also showed that our screening<br />
system <strong>for</strong> detecting anti-‘Mi a ’ has a low sensitivity of 67.2% with a specificity of 93.1%. Since a<br />
previous study suggested the presence of other glycophorin variants in Taiwan, the results suggested the<br />
existence of other (Mi) phenotypes and the current detecting method may not be sufficient to identify<br />
antibodies other than anti-GP. Mur.<br />
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