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Model Amphipathic Peptides 83
relative fluorescence
160
140
120
100
80
60
40
20
0
cytosol
nucleus
online wash online wash online wash online wash online wash online wash
XI XII XIII XIV XV XVI
FIGURE 4.8 Fluorescence intensity of internalized peptides in the cytosol and nucleus after
exposure of LKB Ez7 cells for 30 min at 37°C to 1 µM helical model peptides not related to
I and to the natural vector peptides XV 9 and XVI, 33 with and without subsequent washing
with cold PBS, normalized to the fluorescence intensity of the external peptide solution. Each
bar represents the mean from three cells ± S.E.M.
relative fluorescence
300
250
200
150
100
50
0
I
II
III
V
VI
X
LKB Ez 7 ECV 304 HEK 293 IMCD
FIGURE 4.9 Intensity of the cytosolic fluorescence after exposure of different cell types for
30 min at 37°C to 1 µM I and I-derived peptides, normalized to the fluorescence intensity of
the external peptide solution. Each bar represents the mean from three cells ± S.E.M.
(Figure 4.9; Reference 32). Virtually identical effects to those described earlier for
LKB Ez 7 on the intracellular fluorescence at reduced temperature and after cold
washing were also observed for the other cell types investigated. These observations