Model Amphipathic Peptides 91 16. Hansen, S.H., Sandvig, K., and van-Deurs, B., Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification, J. Cell Biol., 121, 61, 1993. 17. Ohkuma, S. and Poole, B., Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents, Proc. Natl. Acad. Sci. U.S.A., 75, 3327, 1978. 18. Lippincott–Schwartz, J. et al., Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER, Cell, 56, 801, 1989. 19. Cole, S.P. et al., Overex<strong>press</strong>ion of a transporter gene in a multidrug-resistant human lung cancer cell line, Science, 258, 1650, 1992. 20. Ballatori, N. et al., Carrier-mediated uptake of lucifer yellow in skate and rat hepatocytes: a fluid-phase marker revisited, Am. J. Physiol., 277, G896-G904, 1999. 21. Steinman, R.M. et al., Endocytosis and the recycling of plasma membrane, J. Cell Biol., 96, 1, 1983. 22. Schmid, S.L. and Carter, L.L., ATP is required for receptor-mediated endocytosis in intact cells, J. Cell Biol., 111, 2307, 1990. 23. Schnitzer, J.E., Allard, J., and Oh, P., NEM inhibits transcytosis, endocytosis, and capillary permeability: implication of caveolae fusion in endothelia, Am. J. Physiol., 268, H48–H55, 1995. 24. Anderson, R.G. et al., Potocytosis: sequestration and transport of small molecules by caveolae, Science, 255, 410, 1992. 25. Marger, M.D. and Saier, M.H., A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport, Trends Biochem. Sci., 18, 13, 1993. 26. Nikaido, H., Prevention of drug access to bacterial targets: permeability barriers and active efflux, Science, 264, 382, 1994. 27. Schatz, G. and Dobberstein, B., Common principles of protein translocation across membranes, Science, 271, 1519, 1996. 28. Paul, S. et al., Structure and in vitro substrate specificity of the murine multidrug resistance-associated protein, Biochemistry, 35, 13647, 1996. 29. Scheller, A. et al., Structural requirements for cellular uptake of alpha-helical amphipathic peptides, J. Pept. Sci., 5, 185, 1999. 30. Lorenz, D. et al., Mechanism of peptide-induced mast cell degranulation — translocation and patch-clamp studies, J. Gen. Physiol., 112, 577, 1998. 31. Wiesner, B. et al., Measurement of intracellular fluorescence in the presence of a strong extracellular fluorescence using confocal laser scanning microscopy, Anal. Biochem., submitted. 32. Scheller, A. et al., Evidence for an amphipathicity independent cellular uptake of amphipathic cell-penetrating peptides, Eur. J. Biochem., 267, 6043, 2000. 33. Lin, Y.Z. et al., Inhibition of nuclear translocation of transcription factor NF- kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence, J. Biol. Chem., 270, 14255, 1995. 34. Ashkenazi, A. and Dixit, V.M., Death receptors: signaling and modulation, Science, 281, 1305, 1998. 35. Wieland, T., Poisonous principles of mushrooms of the genus Amanita. Four-carbon amines acting on the central nervous system and cell-destroying cyclic peptides are produced, Science, 159, 946, 1968. 36. Faulstich, H. and Wieland, T., New aspects of amanitin and phalloidin poisoning, Adv. Exp. Med. Biol., 391, 309, 1996.
92 Cell-Penetrating Peptides: Processes and Applications 37. Itoh, N. et al., The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis, Cell, 66, 233, 1991. 38. Sato, T. et al., FAP-1: a protein tyrosine phosphatase that associates with Fas, Science, 268, 411, 1995. 39. Kumar, S. and Colussi, P.A., Prodomains-adaptors-oligomerization: the pursuit of caspase activation in apoptosis, Trends Biochem. Sci., 24, 1, 1999. 40. Yanagisawa, J. et al., The molecular interaction of Fas and FAP-1 — a tripeptide blocker of human Fas interaction with FAP-1 promotes Fas-induced apoptosis, J. Biol. Chem., 272, 8539, 1997. 41. Muzio, M. et al., FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex, Cell, 85, 817, 1996. 42. Boldin, M.P. et al., Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death, Cell, 85, 803, 1996. 43. Scheller, A., Melzig, M., and Oehlke, J., Induction of caspase-8 in human cells by the extracellular administration of peptides containing a C-terminal SLV sequence, Lett. Pept. Sci., in <strong>press</strong>. 44. Oehlke, J., Bienert, M., and Faulstich, H., Lett. Pept. Sci., in preparation. 45. Oehlke, J. et al., Cellular uptake of antisense oligonucleotides after complexing or conjugation with cell-penetrating model peptides, Bioconjugate Chem., in preparation. 46. Vaara, M. and Porro, M., Group of peptides that act synergistically with hydrophobic antibiotics against gram-negative enteric bacteria, Antimicrob. Agents Chemother., 40, 1801, 1996. 47. Good, L. et al., Bactericidal antisense effects of peptide-PNA conjugates, Nat. Biotechnol., 19, 360, 2001. 48. Tung, C.H. and Stein, S., Preparation and applications of peptide-oligonucleotide conjugates, Bioconjugate Chem., 11, 605, 2000. 49. Wyman, T.B. et al., Design, synthesis, and characterization of a cationic peptide that binds to nucleic acids and permeabilizes bilayers, Biochemistry, 36, 3008, 1997. 50. Niidome, T. et al., Binding of cationic alpha-helical peptides to plasmid DNA and their gene transfer abilities into cells, J Biol. Chem., 272, 15307, 1997. 51. Pichon, C. et al., Cytosolic and nuclear delivery of oligonucleotides mediated by an amphiphilic anionic peptide, Antisense. Nucleic. Acid. Drug Dev., 7, 335, 1997. 52. Ohmori, N. et al., The enhancing effect of anionic alpha-helical peptide on cationic peptide-mediating transfection systems, Biochem. Biophys. Res. Commun., 235, 726, 1997. 53. Takahashi, S., Conformation of membrane fusion-active 20-residue peptides with or without lipid bilayers. Implication of alpha-helix formation for membrane fusion, Biochemistry, 29, 6257, 1990. 54. Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248, 1976. 55. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods, 65, 55, 1983.
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CELL- PENETRATING PEPTIDES Processe
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Pharmacology and Toxicology: Basic
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Library of Congress Cataloging-in-P
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to the handbook are prominent resea
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REFERENCES 1. Green, M. and Loewens
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Contributors Mats Andersson Microbi
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Erin T. Pelkey Department of Chemis
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Contents Section I Classes of Cell-
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Chapter 16 Cell-Penetrating Peptide
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The Tat-Derived Cell-Penetrating Pe
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The Tat-Derived Cell-Penetrating Pe
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The Tat-Derived Cell-Penetrating Pe
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The Tat-Derived Cell-Penetrating Pe
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The Tat-Derived Cell-Penetrating Pe
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The Tat-Derived Cell-Penetrating Pe
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The Tat-Derived Cell-Penetrating Pe
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The Tat-Derived Cell-Penetrating Pe
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The Tat-Derived Cell-Penetrating Pe
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24 Cell-Penetrating Peptides: Proce
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Interactions of Cell-Penetrating Pe
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Interactions of Cell-Penetrating Pe
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Interactions of Cell-Penetrating Pe
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Interactions of Cell-Penetrating Pe
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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FIGURE 9.7 (Color Figure 9.7 follow
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FIGURE 9.8 The center of the figure
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Structure Prediction of CPPs and It
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Biophysical Studies of Cell-Penetra
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Kinetics of Uptake of Cell-Penetrat
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Cell-Penetrating Peptide Conjugatio
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Cell-Penetrating Peptides as Vector
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TABLE 16.1 Examples of Transport of
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CPP 2 Cargo or mRNA CAP Antisense A
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Microbial Membrane-Permeating Pepti
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Microbial Membrane-Permeating Pepti
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Index A Abaecin, 129 Abz radiolabel
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Index 399 Diffraction, 168 Disulphi
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Index 401 structure prediction, 187
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Index 403 pRB proteins, Tat-E1A bin
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Index 405 lipid perturbation (secon