crc press - E-Lib FK UWKS

Index 403
pRB proteins, Tat-E1A binding of, 370
Predictive and proteome analyses, 310–313
artificial neural network-based methods,
311–312
global structure-based methods, 312–313
prediction algorithms, 310
from proteome to secretome, 313
weight matrix methods, 311
Proline-rich peptides as translocating, 130
Protein fibroblast growth factor-1 (FGF-1), 119
Proteins, transportan and, 61–66
Protein transport, 365–375
delivery of PTD-conjugated macromolecules,
369–373
applications in cell culture, 369–371
applications in vivo, 371–373
development of PTD fusion proteins,
367–369
cloning and purification of Tat fusion
proteins, 368–369
principles of protein transduction, 365–366
protein transduction technology, 366–367
Proteome analyses, 310–313
artificial neural network-based methods,
311–312
global structure-based methods, 312–313
prediction algorithms, 310
from proteome to secretome, 313
weight matrix methods, 311
PR-39 protein, 129
pVEC, 356
Q
Quantification, 263–275
of CPPs via reporter groups, 266–272
biotinylation and cell-ELISA, 267
enzyme activity assays, 270
fluorescence microscopy, spectrometry,
and FACS, 267–269
fluorogenic construct assay (resonance
energy transfer, RET quenching),
270–272
fluorogenic construct assay (resonance
energy transfer, RET),
270–272
HPLC detection, 269–270
immunodetection, 270
radiolabeling, 266–267
of peptide cargo bioactivity, 272–274
DNA/PNA antisense effects, 273
drug activity, 273–274
epidermal growth factor receptor (EGFR),
272–273
principles and background, 263–266
R
Rac GTPases, 370
Radiolabeling
quantification by, 266–267
for uptake kinetics assays, 280–281
125 I-radiolabeling, for uptake kinetics assays,
280–281
Reagents, for solution phase conjugation, 332–336
amine-reactive reagents, 332–333
example: superparamagnetic iron oxide
particle–Tat conjugate, 334–336
heterobifunctional conjugation, 333–334
sulfhydryl-reactive reagents, 333
Receptors, internalization by, 177–179
Resonance energy transfer (RET) quenching,
quantification by, 270–272
Rhodamine membrane uptake assay, 251–252
Rho GTPases, 370
S
Sec6α, 304
Sec61β, 304
Sec61gamma, 304
SecA-dependent bacterial protein secretion
pathways, 299
SecA protein, 299
SecB-dependent bacterial protein secretion
pathways, 298–299
SecB protein, 297–298
SecDFyajC protein, 300
SecE protein, 300
SecG protein, 300
Sec62p, 304
Sec63p, 304–305, 305
Sec71p, 304
SecYEG and related protein-dependent bacterial
protein secretion pathways, 300
SecYEG channel, 297
SecYEG protein, 300
SecY (PrlA) protein, 300
Side effects, 255–256, see also Toxicity
Tat, 256
transportan, 255–256
Signal anchors, 306
Signal peptidases, 306–308
PiHD (XcpA), 307
type I
distribution, 306
specificity, 307
type II, 307
Signal peptides, 295–324
bacterial protein secretion pathways, 297–303
Ffh and 4.5S RNA-dependent, 300–301