crc press - E-Lib FK UWKS

Index
A
Abaecin, 129
Abz radiolabeling, 281–282
AntHD–DNA complex, 206, 208
Antimicrobial activity assays, 385–386
Antimicrobial peptides, 377–396
applications, 388–392
as anti-infective agents, 388–389
as delivery vehicle, 389–392
experimental methods, 385–388
antimicrobial activity assays, 385–386
cell uptake assays, 386–388
cell permeabilization assays, 386–388
fluorescence microscopy and FACS,
386
mechanisms of action, 382–385
cell-killing, 383–385
cell type-specific activities, 385
dual peptide activities, 384–385
intracellular target inhibition, 384
membrane leakage, 383–384
cell uptake, 382–383
cell permeation by cationic peptides,
382–383
receptor-mediated peptide transport
and endocytosis, 383
principles and background, 378–382
composition and structure, 380–382
origins and discovery, 380
Antisense effects, quantification, 273
Apidaecin, 129
Applications, see also specific topics
conjugations and magnetic cell labels, 327–346
microbial membrane-permeating peptides,
377–396
nucleic acid delivery, 347–363
protein transport, 365–375
AP-1 transcription factor, 118
Arginine–proline-rich translocating peptides,
129–130
Arginine-rich peptides
backbone and side chain variations, 141–160
toxicity, 254
Artificial neural networks, 311–312
Atomic surface hydrophobicity, 191–193
Avidin, transportan and, 63–64
B
Bac5, 129
Bac7, 129, 130
Bacillus subtilis, 305, 306
Backbone and side chain variations
discussion, 156–159
materials and methods, 144–147
cellular uptake assays, 146–147
molecular modeling, 146
peptide synthesis, 144
peptoid polyamine synthesis, 145–146
perguanidinylation of peptoid polyamines,
146
robotic peptide synthesis, 144–145
possible permutations, 145
principles and background, 141–144
biological barriers, 141
guanidine headgroup of arginine,
141–143
overview of experiments, 143–144
results, 147–156
backbone conformational freedom and
cellular uptake, 150–156
guanidino peptoid design and cellular
uptake, 147–149
side chain conformational freedom and
cellular uptake, 149–150
Bacterial protein secretion pathways, 297–303
Fhh and 4.55 RNA-dependent, 300–301
FtsY-dependent, 301
SecA-dependent, 299
SecB-dependent, 298–299
SecYEG and related protein-dependent, 300
SRP-dependent, 300
Tat-dependent, 301–302
unknown or factor-independent, 302–303
energetic aspects, 302–303
insertion of procoat proteins, 302
Bacteriorhodopsin, 204
Bicelles, 228–229, 235
Bilayers, in membrane interactions, 169
Bioactivity, quantification in CPP cargoes,
272–274
Biophysical studies, 223–244
biomembrane mimetic solvents and model
systems, 225–229
397