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crc press - E-Lib FK UWKS

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CPP<br />

2<br />

Cargo<br />

or<br />

mRNA<br />

CAP<br />

Antisense<br />

AUG<br />

TAC<br />

AAAAA<br />

Decoy<br />

+<br />

Protein<br />

CPP<br />

-S-S-<br />

4<br />

Cargo<br />

Protein<br />

FIGURE 16.1 Schematic illustration of different CPP–cargo conjugates and their intracellular targets. Cargoes reported and reviewed in this chapter are<br />

ssDNA, dsDNA, ssPNA, ssPS, dsPS, plasmid. A model of transportan structure (From Lindberg, M. et al., Biochemistry, 40, 3141, 2001. With permission.)<br />

is shown as an example of CPP. 1 = denotes the process of internalization of conjugates, 2 = antisense effect of cargo, 3 = antigene effect of cargo, 4 =<br />

competitive binding of the ds decoy oligomer to the DNA-binding proteins (transcription factors, etc.), 5 = transfection with plasmid or phage.<br />

1<br />

Nucleus C ytoplasm<br />

DNA<br />

or or<br />

CPP<br />

3<br />

Antigene<br />

Decoy<br />

Protein ex<strong>press</strong>ion<br />

Plasmid<br />

5<br />

λ<br />

Transfection<br />

CPP<br />

Plasmid<br />

or<br />

CPP<br />

Plasma<br />

membrane<br />

λ phage<br />

Cell-Penetrating Peptides as Vectors for Delivery of Nucleic Acids 359

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