VI Autologous Bone Marrow Transplantation.pdf - Blog Science ...

Table 1. Factors Affecting Quality and Quantity of
Hematopoietic Stem Cells.
1. Cumulative toxicity of previous therapy.
2. Acute toxicity of patient's recent treatment or infection.
3. Dose, timing of hematopoietic growth factors(s) prior to harvest.
4. Sites of bone marrow harvest and quantity removed.
5. Duration and technique of PBSC collection.
6. Freezing, thawing methods and duration of storage.
7. Cancer cell contamination. If purging methods used, toxicity of purging
agents.
Table 2. Clinical Course of 114 Patients with be 1-2 Positive Lymphoma, Purged
with Antibodies, evaluated again. Modified from Gribben et al. NEJM 325:1525,
1991.
bcl-2 by PCR (Subsequent Relapse) Total
BM involved Negative Positive #Pts.
None 35(1) 30 (10) 65
5% 2(1) 9(6) 11
Total # of Pts. 57(4) 57 (26) 114
"True negative" bcl-2 negative, normal morphology; correlation with no subsequent
relapse (short follow-up): 52/55 = 95%
bcl-2 positive, BM involvement; with subsequent relapse:
6/9 = 67%
SIXTH INTERNATIONAL SYMPOSIUM ON AUTOLOGOUS BONE MARROW TRANSPLANTATION 149