VI Autologous Bone Marrow Transplantation.pdf - Blog Science ...

In summary, the ligand for c-kit has biological synergy both in vivo and in
vitro with G-CSF. In vivo, the effects of G-CSF on the absolute leukocyte count
and on the numbers of circulating progenitor cells are markedly enhanced by
the simultaneous administration of rhSCF, but not by prior administration of
rhSCF. In vitro, rhSCF enhances the proliferation of CFC and precursors of CFC
that are stimulated by G-CSF. It is possible that such synergistic interactions may
have clinical importance if circulation of transplantable "stem" cells also is enhanced
or if stem cells in marrow are increased. Further studies to examine the
in vivo synergy of rhSCF with other hematopoietic growth factors may provide
novel therapeutic approaches.
REFERENCES
1. Zsebo KM, Wypych J, et al: Identification, purification, and biological characterization
of hematopoietic stem cell factor from buffalo rat liver-conditioned medium.
Cell 63:195-201,1990.
2. Williams DE, Eisenman J, et al: Identification of a ligand for the c-kit proto-oncogene.
Cell 63:167-174,1990.
3. Huang E, Nocka K, et al: The hematopoietic growth factor KL is encoded at the SI
locus and is the ligand of the c-lit receptor, the gene product of the W locus. Cell
63:225-233,1990.
4. Witte ON: Steel locus defines new multipotent growth factor. Cell 63:5-6,1990.
5. McNiece IK, Langley KE, et al: Recombinant human stem cell factor synergizes with
GM-CSF, G-CSF, IL-3, and epo to stimulate human progenitor cells of the myeloid
and erythroid lineages. Exp Hematol 19:226-231,1991.
6. Carow CE, Hangoc G, et al. Mast cell growth factor (c-kit ligand) supports the
growth of human multipotential progenitor cells with a high replating efficiency.
Blood 78:2216-2221,1991.
7. Bernstein ID, Andrews RG, et al: Recombinant human stem cell factor enhances the
formation of colonies by CD34+ and CD34+ lin- cells, and the generation of colonyforming
cell progeny from CD34+ lin- cells cultured with IL-3, G-CSF, or GM-CSF.
Blood 77:2316-2321,1991.
8. Zsebo KM, Smith KA, et al: Biological characteristics of recombinant rat and human
stem cell factor, in Blood Cell Growth Factors: Their Present and Future Use in
Hematology and Oncology, Murphy MJ (ed), AlphaMed Press, Dayton, OH, 1991,
pl94-203.
9. Andrews RG, Knitter GH, et al: Recombinant human stem cell factor, a c-kit ligand,
stimulates hematopoiesis in primates. Blood 78:1975-1980,1991.
10. Andrews RG, Bartelmez SH, et al: A c-kit ligand, recombinant human stem cell
factor, mediates reversible expansion of multiple CD34+ colony-forming cell types in
blood and marrow of baboons. Blood 80:920-927,1992.
11. Briddell R, Hartley C, et al: The role of stem cell factor in mobilization of peripheral
blood progenitor cells (PBPC) with marrow repopulating ability in mice. Blood
80(Suppl l):12a, 1992 (abstract).
12. Molineux G, Migdalska A, et al: The effects on hematopoiesis of recombinant stem
cell factor (ligand for c-kit) administered in vivo to mice either alone or in combination
with granulocyte colony-stimulating factor. Blood 78:961-966,1991.
13. Sheridan WP, Begley G, et al: Effect of peripheral blood progenitor cells mobilized
by filgrastim (G-CSF) on platelet recovery after high dose chemotherapy. Lancet
339:640-644,1992.
14. Molineux G, Pojda Z, et al. Transplantation potential of peripheral blood stem cells
induced by granulocyte colony-stimulating factor. Blood 76:2153-2158,1990.
230 SIXTH INTERNATIONAL SYMPOSIUM ON AUTOLOGOUS BONE MARROW TRANSPLANTATION