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InnovatIve DIscovery tools For sIgnal transDuctIon research<br />
Pathways In Human Cancer<br />
Pathways In Human Cancer<br />
InnovatIve DIscovery tools For sIgnal transDuctIon research<br />
Immunofluorescence Using Activation~state Antibodies<br />
Immunofluorescence Using Activation~state ...from Cell Signaling Antibodies Technology<br />
• Phospho-p44/42 MAPK (green) • Phospho-Akt (red)<br />
• Phospho-p44/42 MAPK (green) • Phospho-Akt (red)<br />
Untreated LPA, 2 min LPA, 5 min<br />
Untreated LPA, 2 min LPA, 5 min<br />
LPA, 15 min LPA, 15 min + LY294002 LPA, 15 min + U0126<br />
Confocal IF images of Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) #9106<br />
Mouse mAb (green) and Phospho-Akt (Ser473) (193H12) Rabbit mAb<br />
#4058 (red) in C6 rat glioma cells treated with LPA as indicated.<br />
LPA induces cytoplasmic and nuclear phospho-p44/42 MAPK signal<br />
and cytoplasmic and membrane phospho-Akt signal. Addition of MEK<br />
inhibitor U0126 #9903 or PI3K inhibitor LY294002 #9901 completely<br />
blocks activation of phospho-p44/42 MAPK or phospho-Akt,<br />
respectively. Blue pseudocolor = DRAQ5<br />
GSK-3<br />
Confocal IF images of Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) #9106<br />
Mouse mAb (green) and Phospho-Akt (Ser473) (193H12) Rabbit mAb<br />
#4058 (red) in C6 rat glioma cells treated with LPA as indicated.<br />
LPA induces cytoplasmic and nuclear phospho-p44/42 MAPK signal<br />
and cytoplasmic and membrane phospho-Akt signal. Addition of MEK<br />
inhibitor U0126 #9903 or PI3K inhibitor LY294002 #9901 completely<br />
(fluorescent DNA dye).<br />
blocks activation of phospho-p44/42 MAPK or phospho-Akt,<br />
respectively. Blue pseudocolor = DRAQ5<br />
GSK-3<br />
LPA, 15 min LPA, 15 min + LY294002 LPA, 15 min + U0126<br />
(fluorescent DNA dye).<br />
For our Free Pathways<br />
In Human Cancer poster<br />
please<br />
For our<br />
visit<br />
Free<br />
our<br />
Pathways<br />
website:<br />
www.neb-online.de<br />
In Human Cancer poster<br />
please visit our website:<br />
www.neb-online.de<br />
Cyclin D<br />
Cyclin D<br />
p21 p27<br />
p21 p27<br />
FKHR/<br />
FOXO<br />
FKHR/<br />
FOXO<br />
mTOR<br />
Rictor<br />
mTOR<br />
Rictor<br />
MDM2<br />
MDM2<br />
in Deutschland und Österreich exklusiv von<br />
n New England Biolabs GmbH Frankfurt/Main Deutschland Tel.: +49(0)69-305-23140 Fax.: +49(0)69-305-23149 in Deutschland email: und Österreich info@de.neb.com exklusiv www.neb-online.de von<br />
n Cell Signaling Technology Inc. Danvers, MA USA Tel. 1-877-616-CELL (2355) Fax 1-978-867-2488 email: info@cellsignal.com www.cellsignal.com<br />
n New England Biolabs GmbH Frankfurt/Main Deutschland Tel.: +49(0)69-305-23140 Fax.: +49(0)69-305-23149 email: info@de.neb.com www.neb-online.de<br />
n Cell Signaling Technology Inc. Danvers, MA USA Tel. 1-877-616-CELL (2355) Fax 1-978-867-2488 email: info@cellsignal.com www.cellsignal.com<br />
...from Cell Signaling Technology<br />
Akt<br />
Akt<br />
XIAP<br />
PIP 3<br />
PIP 3<br />
Pl3K<br />
Pl3K<br />
c-Raf<br />
c-Raf<br />
XIAP<br />
Bcl-x<br />
Bcl-x<br />
Gab1<br />
Gab2<br />
Gab1<br />
Gab2<br />
PDK1<br />
PDK1<br />
PP2A<br />
PP2A<br />
PAK1<br />
PAK1<br />
RTK<br />
LY294002<br />
14-3-3<br />
Bad<br />
14-3-3<br />
Bad<br />
NEW EN GL AN D<br />
® Bi NEW oL EN abs GL AN D<br />
® Bi oL abs<br />
CytC<br />
GmbH<br />
RTK<br />
LY294002<br />
CytC<br />
GmbH<br />
Halle 9, Stand A24<br />
Halle 9, Stand A24