96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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chemotherapy was recommended and continuing the follow-up observation of the B-cell CLL with no current need for a specific treatment. Conclusions. Despite a challenging combination of simultaneous neoplasias of different and/or the same entit(ies)y, in addition with complicating factors (2 primary tumor manifestations of the same entity with the need of an extended standard surgery due to tumor sites, subileus, perioperatively persisting, initially diagnosed hemoblastosis), the treatment was successful with curative intention and preserved mid- to longterm curative potential by abdominal surgery with a low complication rate. This confirms the indicated primary surgical care of the GI cancers, also because of the coincidence of a colonic carcinoma at 2 sites. The rare transversal-colon intussusception in this age, flanked by a carcinoma of the right and left flexure, is the first report of this type in the literature. FR-P-055 Seltenes Langerhans-Zell-Sarkom der Milz mit ungewöhnlicher klinischer Manifestation J . Arend1 , D . Küster2 , H . Lippert1 , F . Meyer1 1 2 University Hospital, Dept . of Surgery, Magdeburg, University Hospital, Institute of Pathology, Magdeburg Aims. Abklärung eines pathohistolischen Zufallsbefundes nach Splenektomie im Rahmen einer Operation bei Leberparenchymblutung (primär V. a. akute Cholangitis bei Choledochocystolithiasis). Methods. Zunächst therapeutische ERCP mit Stentimplantation im D. hepatocholedochus nach Papillotomie unter antibiotische Abschirmung. In der Folge-CT multiple Leberherde, die zur histologischen Abklärung sonographiegestützt bioptiert wurden. Komplikation: intra- und perihepat. Hämatom, welches bei symptomatischer Größenzunahme und drohendem sept. KH-Bild (Ursache nur teils durch Staphylokokkenkolonisation eines i.v.-Portsystems zur Chemotherapie bei Mamma-Ca erklärt) eine notfallmäßige expl. Laparotomie zur sept. Fokussanierung erforderte: i) Intraabdominal kein sept. Fokus; ii) Entlastung des Leberkapselhämatoms und lokale Thermokoagulation/ Hämostyptikaapplikation; iii) intraop. multiple, unklare, teils massiv blutende fokale Milzläsionen (vulnerable Parenchymoberfläche), die zur Blutungsbeherrschung und pathohistologische Abklärung die Splenektomie erforderten; i.v.) Explantation des infizierten i.v.-Ports. Results. Bei Staphylokokkensepsis (zusätzl. Bakteriämie durch Streptokokkus hominis) und multiplen chologenen Leberabszessen zunächst kalkulierte und später resistenzgerechte Antibiotika (keine klein. Befundbesserung). Im Leberbiopsiepräparat multiple kleine Abszesse mit schaumzellig-histozytärer, resorptiver Entzündungskomponente. Das Splenektomiepräparat war diffus mit zytologisch malignen Zellen bei aufgehobener Milzarchitektur durchsetzt mit einer Zellproliferationsfraktion (Ki-67-Ag) von ca. 50% (pos. Immunhistologie für S-100, CD1a und teils CD68; Lysozym, CD3, Cd20, CD30, Alk 1, Myeloperoxidase und Chlorazetatesterase hingegen negativ). Trotz techn. Operationserfolgs tolerierte die Patientin die Intervention nur mäßig, durch zunehmende AZ-Verschlechterung keine weiterführend ggf. diagnosespezifisch indizierte Therapie möglich. Trotz max. int.-therapeut. Maßnahmen erlag die Patientin 13 Tage nach stationärer Aufnahme dem foudroyanten Verlauf im MOV. Conclusions. Die zur Aufnahme nicht bekannte, seltene Erkrankung eines Langerhans-Zell-Sarkoms trug wesentlich zum fulminanten Cholangitis-Verlauf bei. Zusätzliche Komplikationen wie Portinfektion und Blutung nach Leberpunktion verschlechterten die Prognose weiter. Offen bleibt, ob klinischer Verdacht oder Sarkomfrühdiagnose den KH- Verlauf suffizient beeinflusst hätte. Die Seltenheit der Erkrankung und damit fehlende Erfahrungen in Diagnostik und Therapie erschweren eine individuelle Therapie massiv. Poster: GI-Trakt: GIST, Dünndarm, Kolorektum FR-P-056 Value of epithelioid histomorphology and PDGFRA immunostaining patterns for prediction of PDGFRA mutated genotype in GISTs A . Agaimy1 , C . Otto2 , H . Geddert 3 , I .-M . Schaefer4 , A . Braun2 , R . Schneider- Stock1 , F . Haller2 1 2 Friedrich Alexander University, Institute of Pathology, Erlangen, Albert Ludwigs University, Institute of Pathology, Freiburg, 3St . Vincentius Hospital, Institute of Pathology, Karlsruhe, 4Georg August University, Institute of Pathology, Göttingen Aims. A majority of gastrointestinal stromal tumors (GISTs) carry mutations in the receptor tyrosine kinases KIT or PDGFRA. In a recent study, we demonstrated upregulated expression of KIT in KIT mutated GISTs, in contrast to upregulated PDGFRA expression in PDGFRA mutated GISTs, on mRNA (qRT-PCR) and protein (Western BloT) level. However, most routinely processed GISTs are formalin-fixed and paraffin-embedded; thus, these methods are not applicable in daily pathology routine. Reliable determination of PDGFRA expression by immunohistochemistry might help to identify GISTs with PDGFRA mutation, without the necessity for complete genotyping of KIT and PDGFRA by mutational analysis. The aim of the current study was to evaluate the predictive value of a combination of histomorphology and PDGFRA immunohistochemistry in comparison to mutational analyses. Methods. In order to conduct a tissue microarray, 109 surgically resected GISTs with known mutation status of KIT (74%) and PDGFRA (16%) were used. The histomorphological phenotype (spindled, epithelioid, mixed growth pattern) was determined on H&E sections without knowledge of the genotype. The staining intensity (negative, 1–25%, 26–50%, >50%) and the staining pattern (paranuclear, cytoplasmic, membranous) of PDGFRA were determined without knowledge of the genotype. Results. PDGFRA-mutated GISTs were significantly more often of epithelioid phenotype and had a significantly higher expression of PDGFRA protein, compared to KIT-mutated GISTs. The paranuclear stainig pattern was almost exclusively observed in PDGFRA mutated GISTs. A combination of histomorphology, staining intensity and staining pattern of PDGFRA was a reliable predictor for PDGFRA genotype. Conclusions. A combination of histomorphology and PDGFRA immunostaining is a reliable predictor of PDGFRA genotype. The use of this PDGFRA genotype predictor may help to reduce costs and shorten processing time of GIST genotyping by excluding KIT mutational analysis in PDGFRA-overexpressing GISTs. This might be even more important in less developed countries with restricted health budgets. FR-P-057 Expression of CD34 in GIST is site-dependent and genotypeassociated A . Braun1 , C . Otto1 , H . Geddert2 , D .J . Zhang3 , A . Agaimy4 , Ö . Sahin3 , F . Haller1 1 2 Albert Ludwigs University, Institute of Pathology, Freiburg, St . Vincentius Hospital, Institute of Pathology, Karlsruhe, 3German Cancer Research Center, Heidelberg, 4Friedrich Alexander University, Institute of Pathology, Erlangen Aims. Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gastrointestinal tract. In addition to immunopositivity for KIT (CD117), 60–70% of cases are positive for CD34. CD34 is a cell surface glycoprotein, which is expressed especially in early hematopoietic stem and progenitor cells. Its function is still not yet sufficiently clarified. Our aim was to gain a better understanding of the regulation of CD34 expression in GISTs. Methods. From the archives of our institutes, 109 surgically resected GISTs were compiled. Mutation analyses of KIT (exon 9, 11, 13 and 17), Der Pathologe · Supplement 1 · 2012 | 101
Abstracts PDGFRA (exons 10, 12, 14 and 18), and BRAF (exon 15) were performed. The expression of CD34 was assessed semi-quantitatively using immunohistochemical staining on tissue microarrays. Methylation analyses were performed using pyrosequencing of two CpG loci in the promoter binding region of the CD34 gene (CD34_P339_R and CD34_P780_R). Furthermore, GIST cell lines 882 and 48B were treated with different concentrations of the demethylating agent 5-azacitidine (5-azaC), and the methylation status as well as the mRNA and protein expression of CD34 were determined. Results. KIT-mutated tumors of the stomach and rectum were at both CpG loci of the CD34 gene significantly less methylated compared to KIT-mutated small intestinal GISTs (p
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Inhalt Der Pathologe · Supplement
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Inhalt Der Pathologe · Supplement
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Editorial Liebe Kolleginnen und Kol
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Kolorektales Karzinom 2 VO-005 Tran
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Keynote Lecture VO-014 Genetic dete
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(AMACR, FASN, GOLM1, GSP-pi, ERG) t
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to assess the correct rate of R1 re
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DNA damage, and cytotoxic drugs. Au
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HCV-positive formalin-fixed and par
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well as MET activation were examine
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or BRAF mutation, c-MYC and SIRT1 e
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AG Pneumopathologie III DO-032 Remo
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immature granulopoiesis showed a st
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ned, if well-defined mantle zones,
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phomas). Most prominent gains or am
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DO-062 Tumor-associated macrophages
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DO-080 DOG1: an immunohistochemical
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AG Oralpathologie DO-087 Detection
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DO-094 Do activated fibroblasts inf
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DO-101 Histopathological analysis o
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DO-108 Identification of potential
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Conclusions. In conclusion, 454 par
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subgroup of patients with B-Raf mut
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DO-002b Impact of terminologies in
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Methods. We performed MCPyV-FISH of
Page 52 and 53: FR-013 HPV-genotype distribution in
Page 54 and 55: Methods. Three different techniques
Page 56 and 57: (i.e. investment in equipment and e
Page 58 and 59: FR-032 COLD-PCR: a powerful tool in
Page 60 and 61: dissection (MBLND) technique to imp
Page 62 and 63: SO-005 Prevalence of mutations in s
Page 64 and 65: SO-011 Methylation profiling and in
Page 66 and 67: more effective than SAHA alone and
Page 68 and 69: SO-022 Specialized pathology review
Page 70 and 71: SO-027 Ki-67 in mitotic score group
Page 72 and 73: nificantly associated with advanced
Page 74 and 75: liver did not correlate with the mu
Page 76 and 77: AG Paidopathologie II SO-046 Overex
Page 78 and 79: Results. Histomorphology of the ova
Page 80 and 81: p42 and p27 have not yet been inves
Page 82 and 83: SO-068 Recent advances in understan
Page 84 and 85: SO-075 A novel, dual role of CCN3 i
Page 86 and 87: Pancreatic Adenocarcinoma SG-137 Se
Page 88 and 89: sease and 30 colon cancer serum sam
Page 90 and 91: Conclusions. Although CRC is charac
Page 92 and 93: differentiated and TNM stage III or
Page 94 and 95: pressed moderate levels of the prot
Page 96 and 97: FR-P-037 MALDI imaging reveals COX7
Page 98 and 99: in the context of therapy response
Page 100 and 101: on primary cells of wild-type and c
Page 104 and 105: aims are twofold: 1) to verify the
Page 106 and 107: Results. Her2/neu status in TMAs an
Page 108 and 109: prognostic impact was found in rect
Page 110 and 111: provided information on survival ti
Page 112 and 113: Methods. Biopsy specimens from 430
Page 114 and 115: the OS rate was 53% for patients wi
Page 116 and 117: FR-P-098 Immunohistological stainin
Page 118 and 119: FR-P-104 Histopathological evaluati
Page 120 and 121: within the earliest morphologic det
Page 122 and 123: Conclusions. In primary imaging a g
Page 124 and 125: fibrotic neointma development as we
Page 126 and 127: FR-P-129 Alpha-1-antitrypsin-PiZ-an
Page 128 and 129: FR-P-136 The expression of central
Page 130 and 131: cosa and in the periarterial tissue
Page 132 and 133: FR-P-149 Combination of Castleman d
Page 134 and 135: or without different concentrations
Page 136 and 137: Results. In 18 cases (79%) the caus
Page 138 and 139: FR-P-168 Autopsy findings in a 2-ye
Page 140 and 141: FR-P-174 MPGN-like glomerulonephrit
Page 142 and 143: Poster: Gynäkopathologie und Mamma
Page 144 and 145: SA-P-011 Genetic aberrations of pre
Page 146 and 147: Mechanismen, die eine Progression d
Page 148 and 149: internet server. A network of inter
Page 150 and 151: Methods. HE-gefärbte Schnittpräpa
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Conclusions. The newly released 450
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and desmoplastic reaction are diffe
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one patient revealed more than 9 mi
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situation, the V600E mutation in th
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SA-P-064 Evolution of molecular pat
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nic markers such as myf4 and MyoD1
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Conclusions. To our knowledge, this
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SA-P-085 Expression of the eukaryot
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Results. The papillary RCC type II
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SA-P-098 Male infertility: assessme
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SA-P-104 Process oriented scientifi
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Conclusions. The IBDW offers a uniq
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L Lasitschka F. DO-046 Lehmann A. F
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96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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