96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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DO-080 DOG1: an immunohistochemical marker for neoplastic chondroblasts in chondroblastoma H . Akpalo 1 , C . Lange 2 , J . Zustin 1 1 University Medical Center Hamburg-Eppendorf, Institute of Pathology, Hamburg, 2 University Medical Center Hamburg-Eppendorf, Clinic for Stem Cell Transplantation, Hamburg Aims. Chondroblastoma represents less than 1% of all primary bone tumors and typically presents in the epiphysis of long bones of young patients. The tumor is composed of cartilaginous and osseous matrix along with cellular portions with polygonal chondroblasts with indented nuclei and scattered osteoclast-type multinucleated cells. In the current study, we wished to investigate the expression of several established immunohistochemical markers in the cellular portions of chondroblastomas. Methods. Nine chondroblastomas, seven chondromyxoid fibromas, five giant cell tumors of bone and tissues from five fetal femoral bone endings were analyzed using immunohistochemical antibodies (CD34, SMA, DOG1, CD117, and CD163). Results. The cellular areas of chondroblastoma cases contained nests of DOG1+ SMA+ CD117− CD34− chondroblasts, a phenotype that was not detected in chondromyxoid fibroma cases or in giant cell tumors. The remaining chondroblasts showed only low expression of DOG1 or negative reaction. Furthermore, numerous CD163+ macrophages were detected in all tumors which were analyzed in our study: chondroblastomas, chondromyxoid fibromas and giant cell tumors. Conclusions. We described membranous DOG1+ chondroblasts located within cellular portions of chondroblastoma along with CD163+ macrophages and multinucleated osteoclastic giant cells. Therefore, chondroblastoma can be added to the tumors that are usually positive for DOG1, alongside GIST, rare solid-pseudopapillary neoplasms of the pancreas as well as exceptional mesenchymal tumors including peritoneal leiomyomatosis, uterine type retroperitneal leiomyoma, and synovial sarcoma. DO-081 Bone erosion and inflammation – polymorphonuclear neutrophils promote generation of osteoclasts in osteomyelitis patients M .M . Gaida1 , B . Mayer2 , S . Stegmaier2 , P . Schirmacher1 , C . Wagner3 , G .M . Hänsch2 1 2 University of Heidelberg, Institute of Pathology, Heidelberg, University of Heidelberg, Institute of Immunology, Heidelberg, 3BG Trauma Center Ludwigshafen, Ludwigshafen Aims. Chronic and persistent inflammatory processes in the proximity of bones may lead to severe bone erosion, requiring the amputation of the respective limb. Aim of the present study was to elucidate the process of bone erosion in the context of inflammation. Methods. To explore the relationship between inflammation and bone erosion, biopsies of patients with osteomyelitis due to arterial occlusive disease or to diabetes mellitus were examined (n=31) and the inflammatory infiltrate, bone erosion and infiltration of osteoclasts were quantified. In parallel, interleukin (IL)-8-induced differentiation of CD14+ monocytes derived from the peripheral blood of healthy individuals to osteoclasts was tested in vitro. The cells were cultivated with monocyte colony stimulating factor (M-CSF) and IL-8, and for comparison with the well established osteoclast-inducing receptor activator of Nf κ B ligand (RANKL). To verify the activity of newly generated osteoclasts the ability to degrade ivory slices was tested. The classical pathway of the osteoclastogenesis (NFATc1 and c-fos) was explored by Western blot analysis of isolated cytoplasmic and nuclear proteins after stimulating the isolated monocytes with IL-8. Results. In tissue sections of osteomyelitis patients, in areas of bone destruction, the number of osteoclasts correlated significantly with the extent of the leukocytic infiltrate, particularly with the number of polymorphonuclear neutrophils (PMN). PMN recovered from the infec- ted sites showed characteristics of activation and produced interleukin (IL)-8. CD14 + monocytes derived from the peripheral blood of healthy individuals were cultivated with (M-CSF) and IL-8, and within 3 days, a translocation of the transcription factor NFATc1 into the nucleus was seen, as begin of the differentiation into osteoclasts. By 10 to 20 days, multinucleated cells with the typical osteoclast morphology appeared which expressed tartrate-resistant acid phosphatase (TRAP) and cathepsin K. Moreover, these cells were able to resorb bone. Conclusions. In patients with persistent inflammatory disease and loss of bone, the abundance of PMN in areas of bone resorption correlated with the number of osteoclasts. Since activated PMN are known to produce IL-8, which is able to induce osteoclast formation, we propose that PMN promote bone destruction by local generation of osteoclasts and thus provide a link between the inflammation and bone erosion. DO-082 High IGF2 and FGFR3 are associated with tumor progression in pleomorphic undifferentiated sarcomas, but EGFR and FGFR3 mutations are a rare event K . Rüping1 , D . Katenkamp1 , Y . Chen1 , A . Altendorf Hofmann2 , U . Settmacher2 , I . Petersen1 , T . Knösel1 1 2 Friedrich-Schiller University, Institute of Pathology, Jena, Friedrich-Schiller University, Department of General, Visceral und Vascular Surgery, Jena Aims. Pleomorphic undifferentiated sarcoma (formerly known as malignant fibrous histiocytoma, MFH) is meanwhile recognized as a morphological growth pattern shared by a wide variety of poorly differentiated malignant neoplasms, which include specific subtypes of pleomorphic sarcomas. Nevertheless prognostic and therapeutic options in these tumors are urgently needed. Methods. 327 fibroblastic/myofibroblastic differentiated tumors consisted of 203 pleomorphic undifferentiated sarcomas, 42 low grade sarcomas (10 low grade fibromyxoid sarcoma, 32 low grade myofibroblastic sarcomas) and 82 pseudosarcomatous tumors of the fasciitis family were analyzed immunohistochemically and correlated with clinicopathological parameters. Additionally mutational analysis was performed on high expressed specimens of EGFR and FGFR3. Results. High expression was found in PDGFRA (45%), PDGFRB (35%), EGFR (3.4%), TFE (30%), KDR (1.5%), IGF2 (68%), FGFR1 (6.5%) and FGFR3 (52%). High expression of IGF2 and FGFR3 was significantly correlated with higher tumor grading (low versus high, p5 cm, p
Abstracts study was to evaluate the expression and mutational status of potential molecular therapeutic targets in synovial sarcomas. Methods. 38 well characterized and molecularly confirmed cases of synovial sarcomas were included in this study. Immunohistochemical stainings of tyrosine kinase receptors of the EGF-R family (EGF-R, HER2/ neu, HER3, HER4), the hepatocyte growth factor c-met, and signaling molecules implicated in the mTOR pathway (AKT, mTOR, PTEN), as well as E-Cadherin and snail was performed. In addition, cases were screened for mutations in the EGFR, PIK3C, B-RAF, K-RAS and N-RAS genes. Results. Members oft the EGF-receptor family of kinases as well as E- Cadherin and snail are important for defining the tumor phenotype by determining epithelial-mesenchymal transition of synovial sarcomas. Activation of c-met and signaling molecules oft the mTOR pathway are seen in a significant number of cases. Mutations of the genes studied (EGFR, PIK3C, B-RAF, K-RAS, N-RAS) are an overall rare event in synovial sarcomas. Conclusions. EGF-R expression is found in many synovial sarcomas, however, activating mutations in the tyrosine kinase domain or downstream signaling molecules appear to be a rare event or are absent. Activation of c-met and molecules oft the mTOR pathway is frequently seen in synovial sarcomas. The benefit of targeted therapy against these genes in synovial sarcomas remains to be determined. DO-084 Allergy to metal implants: Immunological und histological analysis of patients with intolerance reaction after knee arthroplasty J . Schneider1 , M . Flaig1 , B . Summer1 , C . von der Helm1 , C . Schopf1 , V . Krenn2 , M . Thomsen3 , L . Frommelt4 , P . Thomas1 1 2 Ludwig-Maximilians-University, Munich, Dermatology, München, Zentrum für Histologie, Zytologie und Molekulare Diagnostik Trier, 3Orthopädische- DRK-Klinik, Baden-Baden, 4Endoklinik, Hamburg Aims. Allergic reaction to metal implants as a reason for implant loosening or other complications is controversially discussed. Therefore we analysed 10 patients with metal allergy but no infection or mechanical problems in knee arthroplasty who needed revision surgery. Methods. In periprosthetic tissue of the 10 patients with metal hypersensitivity (patch testing and/or lymphocyte transformation test (LTT) positive) and CoCrMo based arthroplasty, histological classification (according to consensus classification of periprosthetic interface membranes) and molecular cytokine analysis (Realtime-PCR) was performed. The results were compared with a control group of 5 patients without metal hypersensitivity. After implant replacement to titanium or oxinium based arthroplasty the symptoms were monitored with the WO- MAC-Score. Results. Patch testing: 4/10 reactivity to nickel, 3/10 to cobalt, 1/10 to chromium. Enhanced LTT reactivity: 8/10 to nickel, 1/10 to cobalt. Cytokine expression: IFNy 4/10 vs. 0/5; TGFβ 8/10 vs. 5/5; IL-8 8/10 vs. 0/5; IL-6 6/10 vs. 1/5, IL10 7/10 vs. 5/5. In histopathology primarily the indeterminate type of periprosthetic tissue (type IV) or arthrofibrosis and a varying degree of lymphocytic infiltration were detected. The WOMAC-Score increased from 40.4±20.58 to 55.59±20.14. Conclusions. The combination of allergological, immunological and histopathological diagnostic steps helps to identify patients with implant intolerance reaction and may support the decision to a replacement with alternative material, such as titanium based arthroplasty. 34 | Der Pathologe · Supplement 1 · 2012 DO-085 Expression patterns of microRNA in SFT – prediction of malignancy? C . Poremba1 , C . Altmann1 , N . Arens1 , J . Kriegsmann1 , P . Knöß2 1Research Park Trier, Center of Histopathology, Cytology and Molecular Diagnostics (CHCMD) Trier, Trier, 2Center of Histopathology, Cytology and Molecular Diagnostics Trier Aims. The clinical and biologic behavior of solitary fibrous tumor (SFT) has been a problematic issue, mainly because of inconclusional criteria. SFT harboring “malignant” features such as high cellularity, >4 mitotic figures/10HPF and necrosis/hemorrhage are at risk for metastasis/recurrence. However, in biopsies those criteria may not be evident. In our study, we analyze if expression patterns of microRNA, a class of posttranscriptional regulators, differs between localized, relapsed and metastatic SFT, and may help to predict clinical and biologic behavior in this tumor entity. Methods. In this pilot study, tumor tissues from 6 patients suffering from SFT (3 localized without recurrence/metastasis, 3 with recurrence/metastasis) are analyzed by microRNA assay (Applied Biosystems, Carlsbad, CA USA). Clinical follow-up is available up to 10 years after initial diagnosis. Expression patterns of microRNAs are compared between localized SFT versus relapsed/metastatic SFT and within this group between the initial tumor (areas of different cellularity) and its respective recurrence/metastasis after microdissection. Results. In ongoing analyses, expression patterns of microRNAs are compared between localized vs. relapsed/metastatic SFT and are correlated to clinical outcome. Conclusions. We investigate if different expression patterns of microR- NAs between localized vs. metastatic/relapsed SFT may help to identify patients with higher risk for unfavourable clinical outcome based on the initial biopsy of SFT. Statistical analyses are ongoing. DO-086 Defining arthrofibrosis in histopathological specimens: an evolving concept P . Knöß1 , C . Dierkes1 , M . Ruppert2 , T . Gehrke3 , D . Kendoff3 , C . Theiß4 , V . Krenn1 1Medical health center for histology, cytology and molecular diagnostics Trier, 2Brothers of mercy hospital Trier, 3ENDO Clinic Hamburg, 4Ruhr University Bochum Aims. Arthrofibrosis is the most severe complication in endoprothetic surgery leading to a complete loss of joint function. In the past we reported a proposal for a histopathological grading system for arthrofibrosis (grade 1–3). This time we wanted to verify our results immunohistochemically using β-catenin, a marker known for his association with fibromatosis. Methods. 262 specimens of patients with clinical evidence of arthrofibrosis were graded semiquantitatively for fibroblast density using our proposed grading system, stained with antibodies against β-Catenin and compared with a reference group of 29 neosynovialitis type 4 specimens. Results. In 85.1% of the specimens the histopathologic diagnosis was arthrofibrosis. The distribution for the fibroblast density was 28.8% grade 1, 47.7% grade 2 and 23.4% grade 3. The cellularity was significantly higher in every arthrofibrosis grade than in the reference group (p
Page 2 and 3: Inhalt Der Pathologe · Supplement
Page 4 and 5: Inhalt Der Pathologe · Supplement
Page 6 and 7: Editorial Liebe Kolleginnen und Kol
Page 8 and 9: Kolorektales Karzinom 2 VO-005 Tran
Page 10 and 11: Keynote Lecture VO-014 Genetic dete
Page 12 and 13: (AMACR, FASN, GOLM1, GSP-pi, ERG) t
Page 14 and 15: to assess the correct rate of R1 re
Page 16 and 17: DNA damage, and cytotoxic drugs. Au
Page 18 and 19: HCV-positive formalin-fixed and par
Page 20 and 21: well as MET activation were examine
Page 22 and 23: or BRAF mutation, c-MYC and SIRT1 e
Page 24 and 25: AG Pneumopathologie III DO-032 Remo
Page 26 and 27: immature granulopoiesis showed a st
Page 28 and 29: ned, if well-defined mantle zones,
Page 30 and 31: phomas). Most prominent gains or am
Page 32 and 33: DO-062 Tumor-associated macrophages
Page 36 and 37: AG Oralpathologie DO-087 Detection
Page 38 and 39: DO-094 Do activated fibroblasts inf
Page 40 and 41: DO-101 Histopathological analysis o
Page 42 and 43: DO-108 Identification of potential
Page 44 and 45: Conclusions. In conclusion, 454 par
Page 46 and 47: subgroup of patients with B-Raf mut
Page 48 and 49: DO-002b Impact of terminologies in
Page 50 and 51: Methods. We performed MCPyV-FISH of
Page 52 and 53: FR-013 HPV-genotype distribution in
Page 54 and 55: Methods. Three different techniques
Page 56 and 57: (i.e. investment in equipment and e
Page 58 and 59: FR-032 COLD-PCR: a powerful tool in
Page 60 and 61: dissection (MBLND) technique to imp
Page 62 and 63: SO-005 Prevalence of mutations in s
Page 64 and 65: SO-011 Methylation profiling and in
Page 66 and 67: more effective than SAHA alone and
Page 68 and 69: SO-022 Specialized pathology review
Page 70 and 71: SO-027 Ki-67 in mitotic score group
Page 72 and 73: nificantly associated with advanced
Page 74 and 75: liver did not correlate with the mu
Page 76 and 77: AG Paidopathologie II SO-046 Overex
Page 78 and 79: Results. Histomorphology of the ova
Page 80 and 81: p42 and p27 have not yet been inves
Page 82 and 83: SO-068 Recent advances in understan
Page 84 and 85:
SO-075 A novel, dual role of CCN3 i
Page 86 and 87:
Pancreatic Adenocarcinoma SG-137 Se
Page 88 and 89:
sease and 30 colon cancer serum sam
Page 90 and 91:
Conclusions. Although CRC is charac
Page 92 and 93:
differentiated and TNM stage III or
Page 94 and 95:
pressed moderate levels of the prot
Page 96 and 97:
FR-P-037 MALDI imaging reveals COX7
Page 98 and 99:
in the context of therapy response
Page 100 and 101:
on primary cells of wild-type and c
Page 102 and 103:
chemotherapy was recommended and co
Page 104 and 105:
aims are twofold: 1) to verify the
Page 106 and 107:
Results. Her2/neu status in TMAs an
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prognostic impact was found in rect
Page 110 and 111:
provided information on survival ti
Page 112 and 113:
Methods. Biopsy specimens from 430
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the OS rate was 53% for patients wi
Page 116 and 117:
FR-P-098 Immunohistological stainin
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FR-P-104 Histopathological evaluati
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within the earliest morphologic det
Page 122 and 123:
Conclusions. In primary imaging a g
Page 124 and 125:
fibrotic neointma development as we
Page 126 and 127:
FR-P-129 Alpha-1-antitrypsin-PiZ-an
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FR-P-136 The expression of central
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cosa and in the periarterial tissue
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FR-P-149 Combination of Castleman d
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or without different concentrations
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Results. In 18 cases (79%) the caus
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FR-P-168 Autopsy findings in a 2-ye
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FR-P-174 MPGN-like glomerulonephrit
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Poster: Gynäkopathologie und Mamma
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SA-P-011 Genetic aberrations of pre
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Mechanismen, die eine Progression d
Page 148 and 149:
internet server. A network of inter
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Methods. HE-gefärbte Schnittpräpa
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Conclusions. The newly released 450
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and desmoplastic reaction are diffe
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one patient revealed more than 9 mi
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situation, the V600E mutation in th
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SA-P-064 Evolution of molecular pat
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nic markers such as myf4 and MyoD1
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Conclusions. To our knowledge, this
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SA-P-085 Expression of the eukaryot
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Results. The papillary RCC type II
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SA-P-098 Male infertility: assessme
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SA-P-104 Process oriented scientifi
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Conclusions. The IBDW offers a uniq
Page 176 and 177:
L Lasitschka F. DO-046 Lehmann A. F
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96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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