96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

Abstracts
have been defined, on which experiments can be made in order to differentiate
and pre-sort various types of tissue of the esophagus in histological
recordings using image analysis methods and possibly detecting
conspicuous tissue automatically.
SA-P-107
Computer-based morphological analysis of endomyocardial
structure
M . Schmauder1 , J . Zoschke2 , N .E . Hiemann2 , R . Hetzer2 , R . Meyer2 1 2 Realtime Imaging, Berlin, German Heart Institute Berlin
Aims. Histopathological and immunohistological examinations provide
important information to characterize myocardial tissue. The aim
of computer-based analysis of microscopic biopsy images is to detect
and quantify relevant structures of the myocardium by a standardized
procedure. The results provide the basis for correlative assessment with
clinical results.
Methods. The assessment of interstitial changes is done with Sirius redstained
tissue sections. Fibrous parts are extracted and automatically
measured using an online adjustable, adaptive colour segmentation
method. Immunohistochemical CD31 staining is used to determine the
size and distribution of microvessels. Here, the image analysis consists
of optimized colour segmentation and morphological classification followed
by automated evaluation of area ratios and numerical densities
of capillaries per normalized area. For the analysis of myocardial cells
based on haematoxylin eosin stained samples, a user-guided interactive
process was implemented.
Results. In an interdisciplinary project, we developed a new system for
quantitative morphological image analysis. The system includes three
measurement procedures for the analysis of myocardial structure. The
results are summarized in a combined record. Mean assessment time
is 30–60 min. To date, our preliminary experience has been obtained
in endomyocardial biopsy samples from cardiac transplant recipients.
Conclusions. Our investigations are a step towards a standardized computer-based
analysis of myocardial structure.
SA-P-108
The BMBF Initiative to build up centralized biomaterial banks in
Germany: the BioMaterialBank Heidelberg
E . Herpel1 , R . Kirsten2 , J . Berger2 , C . Döllinger2 , E . Frei3 , C . Ulrich3 ,
P . Schirmacher1 1Institute of Pathology, University Hospital Heidelberg, Heidelberg,
2BioMaterialBank Heidelberg, University Hospital Heidelberg, Heidelberg,
3National Center for Tumor disease
Aims. The BMBF Initiative aims to foster the assembly of centralized
structures for biomaterial banks in Germany, based on site-related
strategic concepts. The BioMaterialBank Heidelberg (BMBH) as one of
5 granted centres will merge all on-site high-quality biomaterial collections
(comprising both tissue and liquid samples) on an administrative
level and integrate them into a consistent project and quality management,
with respect to ethical and legal aspects. The overall aim is to provide
biomaterials or biomaterial collectives in a comprehensive way for
research purpose for researchers in Heidelberg and their cooperation
partners.
Methods. Core of the project is the tissue bank of the National Center
for Tumor Diseases (NCT) Heidelberg, where the essential structures,
regulations and procedures are realized and therefore can serve as a
template. Moreover, other tissue and liquid banks with focus on special,
non-tumorous diseases will be integrated, applying the following
measures:
– Setting up central BMBH administration, including IT/data and
quality management
172 | Der Pathologe · Supplement 1 · 2012
– Further developing and integrating liquid biobanking, thereby adapting
the existing structural concept of the NCT tissue bank
– Further development of a QM assessment program for biomaterials
– Setting up a uniform IT solution for all BMBH modules with optimized
interfaces to material administration
– Completing the biobank technology platform with specific emphasis
on improving the generation of derivatives at BMBH
– Expanding the NCT tissue bank outreach and training Program
Results. The BMBH was initiated in May and started to work in July
2011. The following steps of milestone planning were realized so far:
– Assessment of the current state of all processes, regularities and
structures
– Formal integration of all tissue bank modules into BMBH
– Implementation of a consistent IT-structure (STARLIMS)
Conclusions. In the following years, standardization of processes will be
continued, with a special emphasis on IT- and quality management, and
expanded to all other on-site modules. Thereby, we will lay our focus on
the implementation of a conform quality management for tissue banking,
aiming to accredit all tissue bank modules in year 3 of the grant.
Until the end of the grant period (year 5) all processes, regularities and
structures will be continued, to finally become a highly professional and
sustainable biomaterial bank.
SA-P-109
The BMBF Initiative to build up centralized biomaterial banks in
Germany: the Interdisciplinary Bank of Biomaterials and Data
Würzburg (IBDW)
M . Neumann1 , S . Störk2 , R . Lohmüller3 , S . Kircher4 , A . Rosenwald4 , R . Jahns3 1University of Würzburg, Institute of Clinical Biochemistry and Pathobiochemistry,
Würzburg, 2University of Würzburg, Comprehensive Heart
Failure Center, Würzburg, 3University of Würzburg, Interdisciplinary Bank
of Biomaterials and Data Würzburg, Würzburg, 4University of Würzburg,
Institute of Pathology, Würzburg
Aims. The Interdisciplinary Bank of Biomaterials and Data Würzburg
(IBDW) aims to systematically collect liquid (blood/DNA/urine) and
solid biomaterials (BM) from patients and study participants of the Medical
Campus. One key challenge is to integrate acquisition of BM for
research purposes into clinical routine. Further, BM-collection must
comply with the current legal framework and storage conditions with
current OECD recommendations. BM are linked with corresponding
clinical datasets in accordance with current data protection regulations
and ethical principles securing donor’s privacy.
Methods. The Medical Faculty holds full responsibility for the IBDW
governed by its own steering committee. The IBDW is composed of
3 central units (liquid BM/solid BM/database) and a limited number of
decentralized subunits. All units adhere to IBDW standards and rules.
The build-up process is split into four partly overlapping phases: (1) build
up organisation including patient consent and standard operating
procedures (SOP), (2) build up liquid biobank, (3) build up tissue biobank
(4), implement unified IT infrastructure and interface to the clinical
database. All data within the IBDW will be stored pseudonymized
and are protected by an independent gatekeeper.
Results. Together with the local ethics committee a patient and a proband
consent has been developed. The individual subject donates BM to
the IBDW for future research for an unlimited time period. The consent
allows collecting specified amounts of BM from an individual subject
once within a pre-specified time period. Processing of liquid BM samples
is highly automated to ensure a high quality pre-analytic phase.
All BM is managed by a Biobank Management System which tracks all
handling and processing steps of a sample starting with its acquisition
until storage of the sample or its aliquots in the cryo-repository. Quality
control algorithms are currently implemented. Several existing BM
collections within the University Hospital have been identified to be integrated
into the IBDW.