96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...
96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...
96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...
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FR-P-037<br />
MALDI imaging reveals COX7A2, TAGLN2 and S100-A10 as novel<br />
prognostic markers in Barrett’s Cancer<br />
M . Elsner 1 , S . Rauser 1 , S . Maier 1 , C . Schöne 1 , B . Balluff 1 , S . Meding 1 , G . Jung 1 , M .<br />
Nipp 1 , H . Sarioglu 1 , G . Maccarone 2 , U . Jütting 1 , A . Feuchtinger 1 , R . Langer 3 , M .<br />
Feith 3 , B . Küster 4 , M . Ueffing 1 , H . Zitzelsberger 1 , H . Höfler 3 , A . Walch 1<br />
1 Helmholtz-Zentrum München, Neuherberg, 2 Max Planck Institute of<br />
Psychiatry, München, 3 Technische Universität München, Neuherberg, 4 Technische<br />
Universität München, Chair of Proteomics and Bioanalytics, München<br />
Aims. In or<strong>der</strong> to characterize proteomic changes found in Barrett’s cancer<br />
and its premalignant stages, the proteomic profiles of histologically<br />
proposed precursor and invasive carcinoma lesions were analyzed by<br />
MALDI imaging mass spectrometry (MALDI imaging).<br />
Methods. For a primary proteomic screening, a discovery cohort of 38<br />
fresh frozen Barrett’s Cancer samples was used, with the goal to find intestinal<br />
metaplasia and Barrett’s cancer specific proteins that might be<br />
used as markers for cancer development as well as for lymph node metastasis<br />
and disease outcome. Based on this cohort we studied 33 areas of<br />
Barrett’s Cancer and 11 areas of intestinal metaplasia. Protein identification<br />
was done by mass spectrometry. To validate the identified proteins,<br />
immunohistochemistry was performed on an independent validation<br />
set of 102 Barrett’s Cancer cases and the results were correlated to clinical<br />
data.<br />
Results. We detected 60 m/z species that are significantly differentially<br />
expressed in intestinal metaplasia and invasive carcinoma (p50 IEL/HPF) and mild (