96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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Results. Her2/neu status in TMAs and corresponding whole tissue sections were investigated from the same GCs by two surgical pathologists. 37 (8.2%) and 38 (8.5%) GCs were classified as Her2/neu-positive by the two observers using whole tissue sections. Using the gastric cancer scoring system for biopsies, Her2/neu was positive in 26 (5.9%) and 27 (6.1%) GCs. The interobserver agreement was high (98.5% for whole tissue sections and 98.0% for TMAs; p
Abstracts cessary accuracy in the differentiation of CU associated IEN and could therefore only be used with care in this setting. FR-P-070 Inter-observer variability in the diagnosis of colorectal polyps under special consideration of serrated lesions – a European study T .T . Rau1 , A . Agaimy1 , A . Gehoff2 , C . Geppert1 , K . Jung3 , K . Knobloch1 , C . Langner4 , A . Lugli5 , I . Nagtegaal6 , J . Rüschoff2 , X . Saegert7 , M . Sarbia8 , M . Vieth9 , A . Hartmann1 1 2 University Hospital Erlangen, Institute of Pathology, Erlangen, Institute of Pathology Nordhessen, Kassel, 3Department of medical statistics, Göttingen, 4Medical University Graz, Institute of Pathology, Graz, Austria, 5Uni versity Bern, Institute of Pathology, Bern, Switzerland, 6UDC St . Radboud, Institute of Pathology, Nijmegen, Netherlands, 7Catholic University Leuven, Institute of Pathology, Leuwen, Belgium, 8Institute for Pathology and Cytology, München, 9Institute of Pathology, Bayreuth Aims. A huge number of different criteria in the diagnosis of serrated lesions of the colon are known from the literature. In 2010, a German consensus conference redefined and simplified criteria for the diagnosis of SSA, hyperplastic polyp, traditional serrated adenoma and mixed polyps. As a scientific amendment we aimed to reflect their appliance in daily practice and to prove their ability to achieve inter-observer concordance. Methods. Consecutive colorectal polyps of one month (n=1926) were analysed within a dedicated GI pathology institute (9). All consecutively diagnosed serrated adenomas such as TSA, SSAs and mixed polyps were included. To complete the diagnostic spectrum of colorectal lesions a consecutive number of hyperplastic polyps and classical adenomas were added and completed with a few probes of normal colonic mucosa to reach a study set of 200 lesions. Virtual microscopy was enabled with a Zeiss Mirax Scanner. Hard drives were sent to 10 pathologists with GI experience in 5 European countries in three rounds. The first round blanked, the second providing clinical data, the third round after a conference meeting agreeing upon the recently proposed German consensus guidelines (Virchow’s Archive, 2010 Sep;457(3):291–7). Results. Distribution of gender, age and localization highlighted predominance for certain lesions, but no exclusiveness. Kappa-statistics revealed a basically moderate average agreement (κ=0.56) in the first round. Providing clinical data a slight increase could be achieved (κ=0.63), which was nearly equal to the values under the use of German consensus criteria (κ=0.61). Single criteria of SSA and TSA diagnosis showed a divergence in inter-observer reliability (from κ=0.06 to κ=0.82). Conclusions. Using the simplified German consensus guidelines for serrated lesions the inter-observer certainty of diagnosing serrated lesions is maintained. Using the degree of concordance the single criteria of SSA and TSA diagnosis could be assembled in a hierarchical algorithm. Training in the diagnosis of TSA and mixed polyp seems to be most promising to increase quality in GI pathology. FR-P-071 Role of VEGF-B in the progression of colorectal cancer C . Jayasinghe1 , N . Simiantonaki1 , C .J . Kirkpatrick2 1Gummersbach Hospital, Institute of Pathology, Gummersbach, 2University Medical Center, Institute of Pathology, Mainz Aims. The relevance of VEGF-B, a VEGF (vascular endothelial growth factor) family member, in the progression of colorectal cancer is unclear. Methods. Therefore, using immunohistochemistry we have investigated the expression of this VEGFR (VEGF receptor) -1 ligand in 91 non-metastatic (n=38), lymphogenously metastatic (n=26) and haematogenously metastatic (n=27) colorectal carcinomas. 106 | Der Pathologe · Supplement 1 · 2012 Results. Independent of the metastatic status,VEGF-B was expressed in endothelial as well as epithelial cells in colorectal carcinomas. In 89% of the cases with or without distant metastasis a vascular expression was found. In contrast, only 62% of the nodal-positive tumors had a VEGF-B positive vasculature. In relation to the non-metastatic (p=0.01) and haematogenously metastatic (p=0.027) cases this difference was statistically significant. Concerning the topological staining distribution, in 2/3 of the cases a homogenous pattern was seen without differences in immunostaining between the intratumoral vessels and the vascular fraction throughout the invasive tumor margin. Using the general endothelial marker, CD31, and the lymphatic endothelium-specific marker, D2-40, all VEGF-B positive vessels were of blood but not of lymphatic vascular origin. Interestingly, distal of the tumor a vascular VEGF-B expression was not demonstrable. Positive endothelial VEGF-B expression was not correlated with the metastatic status. In 46% of the investigated cases an epithelial VEGF-B expression was also seen, in 30%, 46% and 67% of the tumors without, with lymph node and with distant metastasis, respectively. In this context positive VEGF-B immunoreactivity was correlated with haematogenous metastasis (p=0.006). The epithelial VEGF-B immunostaining positivity was independent of the grade of the tumor cell dissociation and localization of tumor necrosis. Conclusions. These morphological observations provide evidence for a probable pathobiological significance of VEGF-B in the tumor progression of colorectal cancer, especially in the case of haematogenous metastasis. FR-P-072 Is there a rationale to record lymphatic invasion in node-positive colorectal cancer? N . Schneider1 , J . Betge1 , M .J . Pollheimer1 , R .A . Lindtner1 , P . Kornprat2 , P . Rehak3 , C . Langner1 1 2 Medical University of Graz, Institute of Pathology, Graz, Austria, Medical University of Graz, Department of Surgery, Graz, Austria, 3Medical University of Graz, Research Unit for Biomedical Engineering & Computing, Graz, Austria Aims. The invasion of tumour cells into lymphatic channels represents a crucial step in the metastatic process. The detection of lymphatic invasion in histological slides has been associated with decreased survival and higher recurrence rates. Our study aimed to evaluate prognostic significance of lymphatic invasion in colorectal cancer when lymph node metastasis is already present. Methods. 168 patients with node-positive colorectal cancer (colon, n=98; rectum, n=70) were retrospectively evaluated. Lymphatic invasion was assessed on H&E stained slides. Presence of lymphatic invasion was correlated with different pathological variables applying the chi square test. Progression-free and cancer-specific survivals were assessed using the Kaplan-Meier method. Results. Lymphatic invasion was detected in 95 (57%) cases. There was a significant association of lymphatic invasion with the number of examined tissue blocks: 1–3 blocks (n=50): 46% presence of L1, 4–5 blocks (n=68): 53% presence of L1, and 6 or more blocks (n=50): 72% presence of L1 (p=0.02). Furthermore, L1 was associated with poor tumour differentiation (p=0.009) and the number of involved lymph nodes (p
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Inhalt Der Pathologe · Supplement
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Inhalt Der Pathologe · Supplement
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Editorial Liebe Kolleginnen und Kol
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Kolorektales Karzinom 2 VO-005 Tran
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Keynote Lecture VO-014 Genetic dete
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(AMACR, FASN, GOLM1, GSP-pi, ERG) t
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to assess the correct rate of R1 re
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DNA damage, and cytotoxic drugs. Au
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HCV-positive formalin-fixed and par
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well as MET activation were examine
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or BRAF mutation, c-MYC and SIRT1 e
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AG Pneumopathologie III DO-032 Remo
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immature granulopoiesis showed a st
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ned, if well-defined mantle zones,
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phomas). Most prominent gains or am
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DO-062 Tumor-associated macrophages
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DO-080 DOG1: an immunohistochemical
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AG Oralpathologie DO-087 Detection
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DO-094 Do activated fibroblasts inf
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DO-101 Histopathological analysis o
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DO-108 Identification of potential
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Conclusions. In conclusion, 454 par
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subgroup of patients with B-Raf mut
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DO-002b Impact of terminologies in
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Methods. We performed MCPyV-FISH of
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FR-013 HPV-genotype distribution in
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Methods. Three different techniques
Page 56 and 57: (i.e. investment in equipment and e
Page 58 and 59: FR-032 COLD-PCR: a powerful tool in
Page 60 and 61: dissection (MBLND) technique to imp
Page 62 and 63: SO-005 Prevalence of mutations in s
Page 64 and 65: SO-011 Methylation profiling and in
Page 66 and 67: more effective than SAHA alone and
Page 68 and 69: SO-022 Specialized pathology review
Page 70 and 71: SO-027 Ki-67 in mitotic score group
Page 72 and 73: nificantly associated with advanced
Page 74 and 75: liver did not correlate with the mu
Page 76 and 77: AG Paidopathologie II SO-046 Overex
Page 78 and 79: Results. Histomorphology of the ova
Page 80 and 81: p42 and p27 have not yet been inves
Page 82 and 83: SO-068 Recent advances in understan
Page 84 and 85: SO-075 A novel, dual role of CCN3 i
Page 86 and 87: Pancreatic Adenocarcinoma SG-137 Se
Page 88 and 89: sease and 30 colon cancer serum sam
Page 90 and 91: Conclusions. Although CRC is charac
Page 92 and 93: differentiated and TNM stage III or
Page 94 and 95: pressed moderate levels of the prot
Page 96 and 97: FR-P-037 MALDI imaging reveals COX7
Page 98 and 99: in the context of therapy response
Page 100 and 101: on primary cells of wild-type and c
Page 102 and 103: chemotherapy was recommended and co
Page 104 and 105: aims are twofold: 1) to verify the
Page 108 and 109: prognostic impact was found in rect
Page 110 and 111: provided information on survival ti
Page 112 and 113: Methods. Biopsy specimens from 430
Page 114 and 115: the OS rate was 53% for patients wi
Page 116 and 117: FR-P-098 Immunohistological stainin
Page 118 and 119: FR-P-104 Histopathological evaluati
Page 120 and 121: within the earliest morphologic det
Page 122 and 123: Conclusions. In primary imaging a g
Page 124 and 125: fibrotic neointma development as we
Page 126 and 127: FR-P-129 Alpha-1-antitrypsin-PiZ-an
Page 128 and 129: FR-P-136 The expression of central
Page 130 and 131: cosa and in the periarterial tissue
Page 132 and 133: FR-P-149 Combination of Castleman d
Page 134 and 135: or without different concentrations
Page 136 and 137: Results. In 18 cases (79%) the caus
Page 138 and 139: FR-P-168 Autopsy findings in a 2-ye
Page 140 and 141: FR-P-174 MPGN-like glomerulonephrit
Page 142 and 143: Poster: Gynäkopathologie und Mamma
Page 144 and 145: SA-P-011 Genetic aberrations of pre
Page 146 and 147: Mechanismen, die eine Progression d
Page 148 and 149: internet server. A network of inter
Page 150 and 151: Methods. HE-gefärbte Schnittpräpa
Page 152 and 153: Conclusions. The newly released 450
Page 154 and 155: and desmoplastic reaction are diffe
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one patient revealed more than 9 mi
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situation, the V600E mutation in th
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SA-P-064 Evolution of molecular pat
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nic markers such as myf4 and MyoD1
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Conclusions. To our knowledge, this
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SA-P-085 Expression of the eukaryot
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Results. The papillary RCC type II
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SA-P-098 Male infertility: assessme
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SA-P-104 Process oriented scientifi
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Conclusions. The IBDW offers a uniq
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L Lasitschka F. DO-046 Lehmann A. F
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96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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