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96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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FR-P-149<br />

Combination of Castleman disease and Langerhans cell histiocytosis<br />

in a supraclavicular lymph node<br />

H . Ged<strong>der</strong>t 1 , A . Dimmler 1 , U . Götschin 2 , M . Schatz 3 , G . Faller 1<br />

1 St . Vincent Hospital, Institute of Pathology, Karlsruhe, 2 St . Vincent Hospital,<br />

Department of General Surgery, Karlsruhe, 3 St . Vincent Hospital, Department<br />

of Hematology and Oncology, Karlsruhe<br />

Aims. A 51-year-old female patient presented to our hospital with a 5 cm<br />

measuring mass in the right supraclavicular fossa. Because of known<br />

nodular goiter an ectopic thyroid nodule was suspected. The patient was<br />

in good clinical condition without B symptoms. To exclude malignant<br />

lymphoma the whole mass was excised.<br />

Methods. During intraoperative frozen section suspicion of Castleman<br />

disease was raised, although a second cellular component remained unclear.<br />

Results. After routine tissue processing diagnosis of Castleman’s disease<br />

of hyaline-vascular subtype in a lymph node was confirmed. The second<br />

component emerged as Langerhans cell histiocytosis. No signs of multicentric<br />

Castleman disease or its associated conditions were found. Systemic<br />

Langerhans cell histiocytosis was excluded clinically.<br />

Conclusions. One year after diagnosis the patient is still in good condition<br />

without any sign of relapse. To our knowledge, this is the first report of a<br />

combination of Castleman disease and Langerhans cell histiocytosis in<br />

a single lymph node.<br />

FR-P-150<br />

Notch2, possible a crucial gene in pathogenesis in lymphoma<br />

X .-x . Zhang1 , Q . Lai1 , R . Zhou1 1Zhejiang University, School of Medicine, Hangzhou, China<br />

Aims. In mammals, there are four kinds of Notch transmembrane receptors<br />

(Notch1 to 4); and there are many kinds of Notch target genes<br />

include Hes family, nuclear factor kappaB (NF-κB), cyclin D1, c-myc,<br />

p21, p27, etc. In recent, researches show that Notch2 signaling may be an<br />

important regulator of B cell lymphocytes activation and effect cell differentiation.<br />

However, the functions of Notch2 in lymphoma remain poorly<br />

un<strong>der</strong>stood. In our study, we try to investigate the role which Notch2<br />

plays in the pathogenesis of lymphoma, especially on B cell lymphoma.<br />

Methods. First, Notch2 was detected in 95 B cell lymphoma samples<br />

through PCR, and DNA sequencing was used to see if there were mutations<br />

or not. Then the pEYFP-n1, which contains the Notch2 gene or not,<br />

was transfected into the lymphoma cell lines (include raji, ramos, daudi,<br />

jurkat and molt4) and 293T cell by electro-transfection. And then total<br />

RNA and protein were extracted from the transfected cells, and then<br />

the expression levels of targeted genes, such as c-myc, cyclinD1, p21, p27,<br />

bcl6, p50 and p65, were detected by real-time PCR or Western blot. Statistical<br />

analysis was done by the Student’s t-test between the test groups<br />

and the control group. A P-value of

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