96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...
96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...
96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...
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FR-P-149<br />
Combination of Castleman disease and Langerhans cell histiocytosis<br />
in a supraclavicular lymph node<br />
H . Ged<strong>der</strong>t 1 , A . Dimmler 1 , U . Götschin 2 , M . Schatz 3 , G . Faller 1<br />
1 St . Vincent Hospital, Institute of Pathology, Karlsruhe, 2 St . Vincent Hospital,<br />
Department of General Surgery, Karlsruhe, 3 St . Vincent Hospital, Department<br />
of Hematology and Oncology, Karlsruhe<br />
Aims. A 51-year-old female patient presented to our hospital with a 5 cm<br />
measuring mass in the right supraclavicular fossa. Because of known<br />
nodular goiter an ectopic thyroid nodule was suspected. The patient was<br />
in good clinical condition without B symptoms. To exclude malignant<br />
lymphoma the whole mass was excised.<br />
Methods. During intraoperative frozen section suspicion of Castleman<br />
disease was raised, although a second cellular component remained unclear.<br />
Results. After routine tissue processing diagnosis of Castleman’s disease<br />
of hyaline-vascular subtype in a lymph node was confirmed. The second<br />
component emerged as Langerhans cell histiocytosis. No signs of multicentric<br />
Castleman disease or its associated conditions were found. Systemic<br />
Langerhans cell histiocytosis was excluded clinically.<br />
Conclusions. One year after diagnosis the patient is still in good condition<br />
without any sign of relapse. To our knowledge, this is the first report of a<br />
combination of Castleman disease and Langerhans cell histiocytosis in<br />
a single lymph node.<br />
FR-P-150<br />
Notch2, possible a crucial gene in pathogenesis in lymphoma<br />
X .-x . Zhang1 , Q . Lai1 , R . Zhou1 1Zhejiang University, School of Medicine, Hangzhou, China<br />
Aims. In mammals, there are four kinds of Notch transmembrane receptors<br />
(Notch1 to 4); and there are many kinds of Notch target genes<br />
include Hes family, nuclear factor kappaB (NF-κB), cyclin D1, c-myc,<br />
p21, p27, etc. In recent, researches show that Notch2 signaling may be an<br />
important regulator of B cell lymphocytes activation and effect cell differentiation.<br />
However, the functions of Notch2 in lymphoma remain poorly<br />
un<strong>der</strong>stood. In our study, we try to investigate the role which Notch2<br />
plays in the pathogenesis of lymphoma, especially on B cell lymphoma.<br />
Methods. First, Notch2 was detected in 95 B cell lymphoma samples<br />
through PCR, and DNA sequencing was used to see if there were mutations<br />
or not. Then the pEYFP-n1, which contains the Notch2 gene or not,<br />
was transfected into the lymphoma cell lines (include raji, ramos, daudi,<br />
jurkat and molt4) and 293T cell by electro-transfection. And then total<br />
RNA and protein were extracted from the transfected cells, and then<br />
the expression levels of targeted genes, such as c-myc, cyclinD1, p21, p27,<br />
bcl6, p50 and p65, were detected by real-time PCR or Western blot. Statistical<br />
analysis was done by the Student’s t-test between the test groups<br />
and the control group. A P-value of