the OS rate was 53% for patients with a mo<strong>der</strong>ate and 33% for patients with a poor regression (p=0.08). Other factors such as gen<strong>der</strong>, total tumour count and size, nodal status, localisation of the primaries, as well as duration and mode of NACT did not correlate in uni- or multivariate analysis with OS. Multivariate analysis showed an associated correlation between overall-resection (p
Abstracts Results. Amplification of the FGFR1 region was rare, but could be found in three of 128 cases of pancreatic ductal adenocarcinoma (3/128; 2.3%) in our collective. Slightly more protein expression of FGFR1 was observed with commercially available FGFR1 antibodies by standard immunohistochemistry. So far, none of the pancreatic carcinoma cell lines showed FGFR1 amplification. Conclusions. FGFR1 amplification can be identified in a small subset of pancreatic adenocarcinomas. FGFR1 is a tyrosine kinase receptor protein which is able to promote tumor progression by altering angiogenesis, tumor cell proliferation, migration and cell survival. In the future, it will be necessary to characterize the biological role of FGFR1 amplification in pancreatic adenocarcinoma. Further studies are needed to determine, whether the patients with FGFR1 amplification and/or overexpression need to be pre-selected prior to TKI treatment. FR-P-095 Paraduodenal pancreatitis: mini-series with regard to vessel obliteration T . Hansen1 , F . Vitali2 , R . Kießlich3 , S . Heinrich4 , P . Mildenberger5 , A . Kumar5 , L . Frulloni2 , C .J . Kirkpatrick1 1 2 University of Mainz, Institute of Pathology, Mainz, University of Verona, Department of Medicine, Verona, Italy, 3University of Mainz, Department of Internal Medicine, Mainz, 4University of Mainz, Department of General and Abdominal Surgery, Mainz, 5University of Mainz, Department of Radiology, Mainz Aims. Paraduodenal pancreatitis comprises a form of chronic pancreatitis involving the duodenal wall close to the minor papilla within the surrounding parenchymal tissue and common bile duct (also called groove area). This disor<strong>der</strong> most commonly affects male patients in the 5th decade with a history of alcohol and/or nicotine abuse. Concerning the pathogenesis, it is believed that alcohol or smoking leads to a resistance of the pancreatic juice flow and ischemia of the paraduodenal tissue, giving relapsing episodes of pancreatitis. We report on a series of patients with paraduodenal pancreatitis with emphasis on vascular changes. Methods. We describe ten patients (all male, mean age 45.4 yrs). Most of them presented with abdominal pain (n=8), and weight loss was additionally found in eight patients as well. All patients were smokers; history of alcohol abuse could be confirmed in seven cases. In all patients, a pancreatico-duodenectomy was performed. For histological evaluation, tissue specimens were routinely processed. Results. The following characteristic histological phenomena of paraduodenal pancreatitis were observed: Brunner’s gland hyperplasia occurred in all cases, while cystic changes of the duodenal wall and adenomyomatosis of the duodenal wall were found in 9/10 patients. Variable numbers of a mixed inflammatory infiltrate were present in all patients analyzed. In 50%, we found foreign body giant cell reaction in the neighbourhood of some pseudocysts. However, most interestingly obliteration of segmental arteries was present in 6/10 cases. Conclusions. This histological study confirms the common morphological changes in paraduodenal pancreatitis. Interestingly, we found vessel obliteration in several cases, which has not been described for this subtype of chronic pancreatitis so far. It remains to be investigated whether this specific finding might reflect a particular subgroup of paraduodenal pancreatitis. 114 | Der Pathologe · Supplement 1 · 2012 FR-P-096 Diagnostic value of immunohistochemical IMP3 expression in core needle biopsies of pancreatic ductal adenocarcinoma D .L . Wachter1 , A . Schlabrakowski2 , J . Hoegel3 , G . Kristiansen4 , A . Hartmann1 , M .-O . Riener1 1University Hospital Erlangen-Nuremberg, Institute of Pathology, Erlangen, 2 3 Nuremberg Clinic Center, Insitute of Pathology, Institute of Human Genetics, University Hospital Ulm, 4University Hospital Zurich, Zürich, Switzerland Aims. The oncofetal protein, insulin-like growth factor-II messenger ribonucleic acid-binding protein 3 (IMP3), has been analyzed in many different tumors. Various studies have found that IMP3 is a marker for malignancy and is correlated with increased tumor aggressiveness and reduced overall survival. The diagnosis of pancreatic ductal adenocarcinoma (PDAC) in core needle biopsies can be challenging, and immunohistochemical markers are needed. Methods. We studied IMP3 expression in 177 core needle biopsies of the pancreas, including 112 PDACs, 55 cases with chronic sclerosing pancreatitis, and 10 biopsies with tumor-free pancreatic tissue without inflammation. An additional 18 biopsies of PDAC metastases (16 liver biopsies and 2 lymph node biopsies) were analyzed. To study IMP3 expression in large tissue sections, 45 pancreatic resection specimens (26 with PDAC and 19 with chronic sclerosing pancreatitis) were investigated. Results. In contrast to normal or inflamed pancreatic tissue, which was negative in 47 of 65 (72.3%) cases and weakly positive in 15 of 65 (23.1%) cases, strong IMP3 expression was found in 99 of 112 (88.4%) PDACs. Therefore, sensitivity and specificity of IMP3 expression in the differential diagnosis of PDAC and chronic sclerosing pancreatitis using core needle biopsies were found to be 88.4% and 94.6%, respectively. These results were confirmed in the pancreas resection specimens. Furthermore, strong IMP3 expression was found in 17 of 18 (94.4%) of the PDAC metastases that were analyzed. Conclusions. Our study shows that IMP3 is an easy to use and potentially new immunohistochemical marker for the diagnosis of PDAC in core needle biopsies. FR-P-097 Molecular analysis of putative therapeutic targets in pancreatic acinar cell carcinomas F . Bergmann1 , H . Bläker2 , A . Harjung1 , P . Mayer1 , B . Sipos3 , G . Klöppel4 , P . Schirmacher1 1 2 University of Heidelberg/ Institute of Pathology, Heidelberg, Charité Berlin, Institute of Pathology, 3University of Tübingen, Institute of Pathology, 4Tech nical University Munich, Institute of Pathology Aims. Pancreatic acinar cell carcinomas represent aggressive tumors who frequently display metastases at the time of diagnosis. Because they are rare, established therapeutic concepts other than surgery practically do not exist. The aim of the present study was to evaluate established and innovative therapeutic markers in these to date poorly un<strong>der</strong>stood tumors. Methods. Putative therapeutic targets were investigated by means of direct sequencing, immunohistochemistry, and/or Western blot analyses in a series of 60 pancreatic acinar cell carcinomas. Results. 4% of the tumors displayed k-ras mutations. While 40% of the acinar cell carcinomas showed immunohistochemical expression of EGFR, no EGFR mutations were detected. Heat shock protein 90, heat shock protein 70, L1CAM, and Her2/neu were expressed in 100%, 90%, 65%, and 0% of the tumors, each. mgMT deficiency was found in 28% of the tumors. Conclusions. EGFR, heat shock proteins 90 and 70, L1CAM, and mgMT represent promising targets and predictive markers for the therapy of inoperable or progressive pancreatic acinar cell carcinomas.
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Inhalt Der Pathologe · Supplement
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Inhalt Der Pathologe · Supplement
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Editorial Liebe Kolleginnen und Kol
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Kolorektales Karzinom 2 VO-005 Tran
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Keynote Lecture VO-014 Genetic dete
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(AMACR, FASN, GOLM1, GSP-pi, ERG) t
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to assess the correct rate of R1 re
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DNA damage, and cytotoxic drugs. Au
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HCV-positive formalin-fixed and par
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well as MET activation were examine
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or BRAF mutation, c-MYC and SIRT1 e
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AG Pneumopathologie III DO-032 Remo
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immature granulopoiesis showed a st
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ned, if well-defined mantle zones,
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phomas). Most prominent gains or am
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DO-062 Tumor-associated macrophages
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DO-080 DOG1: an immunohistochemical
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AG Oralpathologie DO-087 Detection
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DO-094 Do activated fibroblasts inf
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DO-101 Histopathological analysis o
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DO-108 Identification of potential
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Conclusions. In conclusion, 454 par
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subgroup of patients with B-Raf mut
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DO-002b Impact of terminologies in
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Methods. We performed MCPyV-FISH of
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FR-013 HPV-genotype distribution in
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Methods. Three different techniques
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(i.e. investment in equipment and e
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FR-032 COLD-PCR: a powerful tool in
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dissection (MBLND) technique to imp
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SO-005 Prevalence of mutations in s
- Page 64 and 65: SO-011 Methylation profiling and in
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- Page 82 and 83: SO-068 Recent advances in understan
- Page 84 and 85: SO-075 A novel, dual role of CCN3 i
- Page 86 and 87: Pancreatic Adenocarcinoma SG-137 Se
- Page 88 and 89: sease and 30 colon cancer serum sam
- Page 90 and 91: Conclusions. Although CRC is charac
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- Page 106 and 107: Results. Her2/neu status in TMAs an
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- Page 122 and 123: Conclusions. In primary imaging a g
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- Page 126 and 127: FR-P-129 Alpha-1-antitrypsin-PiZ-an
- Page 128 and 129: FR-P-136 The expression of central
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- Page 132 and 133: FR-P-149 Combination of Castleman d
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- Page 136 and 137: Results. In 18 cases (79%) the caus
- Page 138 and 139: FR-P-168 Autopsy findings in a 2-ye
- Page 140 and 141: FR-P-174 MPGN-like glomerulonephrit
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- Page 144 and 145: SA-P-011 Genetic aberrations of pre
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- Page 148 and 149: internet server. A network of inter
- Page 150 and 151: Methods. HE-gefärbte Schnittpräpa
- Page 152 and 153: Conclusions. The newly released 450
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Conclusions. To our knowledge, this
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SA-P-085 Expression of the eukaryot
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Results. The papillary RCC type II
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SA-P-098 Male infertility: assessme
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SA-P-104 Process oriented scientifi
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Conclusions. The IBDW offers a uniq
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L Lasitschka F. DO-046 Lehmann A. F
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