96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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DO-101 Histopathological analysis of proteases in abdominal aortic aneurysm wall J . Pelisek 1 , M . Rudelius 2 , C . Reeps 1 , F . Lohoefer 1 , C . Lipp 1 , H .-H . Eckstein 1 1 Klinikum rechts der Isar der TU München, Clinic of Vascular Surgery, München, 2 Klinikum rechts der Isar der TU München, Institute of Pathology, München Aims. Abdominal aortic aneurysm (AAA) wall is characterised by degradation of extracellular matrix through plethora of proteases. In contrast to the already well explored matrix metalloproteinases (MMPs), little is known about other groups, such as ADAM family of metalloproteases (a disintegrin and metalloprotease) or cathepsins. The aim of the study was therefore a detailed analysis of expression of selected ADAMs and cathepsins with known proteolytic activity of relevant extracellular components of the vessel wall and their inhibitors in specimens of patients with AAA. Methods. Tissue samples of vessel wall of 35 AAA patients and 10 organ donors were analysed by immunohistochemistry for expression of MMP-1, -2, -3, -7, -8, -9, -12, -13, ADAM 8, 9, 10, 12, 15, 17, and cathepsin B, D, K, L, S in all cells located within AAA. In addition, the known inhibitors of these proteases TIMP-1, -3, and cystatin C were analysed. Results. Endothelial cells (ECs) were positive for MMP-1, -3, -9, neovessels expressed all MMPs tested except for MMP-13. Aortic medial smooth muscle cells (SMCs) expressed MMP-1,-2,-3,-9. Inflammatory infiltrates expressed all MMPs tested except for MMP-2, macrophages expressed all MMPs. ADAMs were expressed in both AAA and control aorta without any significant differences between the groups. SMCs, neovessels, and macrophages were positive for all ADAMs tested. ECs of AAA were positive for cathepsin D and partially for cathepsin B, K, and S. Macrophages, neovessels and SMCs were positive for all cathepsins tested. Inflammatory infiltrates expressed all cathepsins in the following manner: D>B=S>K
Abstracts Conclusions. The AIM2 expression and induction patterns suggest a role in vascular pathogenesis. AIM2 might act as a danger signal in vascular EC, SMC and infiltrating inflammatory cells. DO-104 Carbamylated EPO-fusion protein and recombinant human EPO during porcine kidney I/R injury F . Simon1 , M . Gröger2 , O . McCook2 , E . Calcia2 , P . Radermacher2 , H . Schelzig1 1 2 University of Düsseldorf, University of Ulm, Ulm Aims. A newly carbamylated erythropoietin-fusion protein (cEPO-FC) and recombinant human EPO (rhEPO) equally protected against spinal cord I/R injury in young, healthy swine [1]. In a recent clinical trial, however, rh-EPO did not affect acute kidney injury in ICU patients [2]. Since patients often present with vascular disease and consecutive organ dysfunction, we compared cEPO-FC and rh-EPO in swine with ubiquitous atherosclerosis [3]. Methods. Pigs randomly received either of cEPO-FC (50 μg/kg), rh-EPO (5000 IU/kg) or vehicle twice over 30 min before and during the first 4 h of reperfusion after 120 min of aortic occlusion using inflatable balloons. We assessed creatinine-clearance, fractional Na+ excretion, blood NGAL, cytokine and NO levels together with tissue histology and immune-histochemistry and -blotting for iNOS, HO-1, HIF-1α , NF-κB, and markers of apoptosis. Results. All pigs presented with reduced glomerular filtration (creatinine-clearance 74±24 vs. 90–140 mL/min normal value) and pre-existing histological organ damage. Neither cEPO-FC nor rh-EPO beneficially influenced the I/R-induced kidney dysfunction, histological organ damage nor tissue inflammation and apoptosis. Conclusions. Pre-existing atherosclerosis-induced kidney dysfunction and tissue damage may reduce the efficacy of cEPO-FC and rh-EPO to prevent I/R-induced kidney damage. References 1 . Simon F et al (2011) . Intensive Care Med, in press 2 . Thim T et al (2010) . EuroIntervention 6:261–8 3 . Endre Z et al (2010) . Kidney Int 77:1020–30 DO-106 Antibody-mediated rejection in cardiac transplant recipients is a seasonal disease K . Wassilew1 , N .E . Hiemann1 , D . Kemper1 , R . Hetzer1 1Deutsches Herzzentrum Berlin, Berlin Aims. Diagnosis of antibody-mediated rejection (AMR) is still a matter of controversial discussion. We suggested that staining for immunoglobulins might improve the diagnostic spectrum of AMR because of seasonal effects in complement deposition. Methods. We studied prospectively all endomyocardial biopsies harvested since 01/2011 (n=205) for acute cellular rejection, activated endothelial-cells and deposition of C4d, C3d and IgA/M/G in interstitial capillaries by paraffin immunohistochemistry. Histologic and immunohistochemical parameters of AMR were classified according to the ISHLT and studied for seasonal effects in an ordinal (Jan–Mar vs. Apr– Jun vs. Jul–Sept vs. Oct–Dec) and nominal model (Oct–Mar vs. Apr– Sept). Results. Overall, 16% of biopsies showed signs of acute cellular rejection of any grade and 46% of samples showed evidence of endothelial-cell swelling. In the ordinal model (Jan–Mar vs. Apr–Jun vs. Jul–Sep vs. Oct–Dec), seasonal effects were found for endothelial cell swelling (83% vs. 14% vs. 49% vs. 40%; p
Page 2 and 3: Inhalt Der Pathologe · Supplement
Page 4 and 5: Inhalt Der Pathologe · Supplement
Page 6 and 7: Editorial Liebe Kolleginnen und Kol
Page 8 and 9: Kolorektales Karzinom 2 VO-005 Tran
Page 10 and 11: Keynote Lecture VO-014 Genetic dete
Page 12 and 13: (AMACR, FASN, GOLM1, GSP-pi, ERG) t
Page 14 and 15: to assess the correct rate of R1 re
Page 16 and 17: DNA damage, and cytotoxic drugs. Au
Page 18 and 19: HCV-positive formalin-fixed and par
Page 20 and 21: well as MET activation were examine
Page 22 and 23: or BRAF mutation, c-MYC and SIRT1 e
Page 24 and 25: AG Pneumopathologie III DO-032 Remo
Page 26 and 27: immature granulopoiesis showed a st
Page 28 and 29: ned, if well-defined mantle zones,
Page 30 and 31: phomas). Most prominent gains or am
Page 32 and 33: DO-062 Tumor-associated macrophages
Page 34 and 35: DO-080 DOG1: an immunohistochemical
Page 36 and 37: AG Oralpathologie DO-087 Detection
Page 38 and 39: DO-094 Do activated fibroblasts inf
Page 42 and 43: DO-108 Identification of potential
Page 44 and 45: Conclusions. In conclusion, 454 par
Page 46 and 47: subgroup of patients with B-Raf mut
Page 48 and 49: DO-002b Impact of terminologies in
Page 50 and 51: Methods. We performed MCPyV-FISH of
Page 52 and 53: FR-013 HPV-genotype distribution in
Page 54 and 55: Methods. Three different techniques
Page 56 and 57: (i.e. investment in equipment and e
Page 58 and 59: FR-032 COLD-PCR: a powerful tool in
Page 60 and 61: dissection (MBLND) technique to imp
Page 62 and 63: SO-005 Prevalence of mutations in s
Page 64 and 65: SO-011 Methylation profiling and in
Page 66 and 67: more effective than SAHA alone and
Page 68 and 69: SO-022 Specialized pathology review
Page 70 and 71: SO-027 Ki-67 in mitotic score group
Page 72 and 73: nificantly associated with advanced
Page 74 and 75: liver did not correlate with the mu
Page 76 and 77: AG Paidopathologie II SO-046 Overex
Page 78 and 79: Results. Histomorphology of the ova
Page 80 and 81: p42 and p27 have not yet been inves
Page 82 and 83: SO-068 Recent advances in understan
Page 84 and 85: SO-075 A novel, dual role of CCN3 i
Page 86 and 87: Pancreatic Adenocarcinoma SG-137 Se
Page 88 and 89: sease and 30 colon cancer serum sam
Page 90 and 91:
Conclusions. Although CRC is charac
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differentiated and TNM stage III or
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pressed moderate levels of the prot
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FR-P-037 MALDI imaging reveals COX7
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in the context of therapy response
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on primary cells of wild-type and c
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chemotherapy was recommended and co
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aims are twofold: 1) to verify the
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Results. Her2/neu status in TMAs an
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prognostic impact was found in rect
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provided information on survival ti
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Methods. Biopsy specimens from 430
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the OS rate was 53% for patients wi
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FR-P-098 Immunohistological stainin
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FR-P-104 Histopathological evaluati
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within the earliest morphologic det
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Conclusions. In primary imaging a g
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fibrotic neointma development as we
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FR-P-129 Alpha-1-antitrypsin-PiZ-an
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FR-P-136 The expression of central
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cosa and in the periarterial tissue
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FR-P-149 Combination of Castleman d
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or without different concentrations
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Results. In 18 cases (79%) the caus
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FR-P-168 Autopsy findings in a 2-ye
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FR-P-174 MPGN-like glomerulonephrit
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Poster: Gynäkopathologie und Mamma
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SA-P-011 Genetic aberrations of pre
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Mechanismen, die eine Progression d
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internet server. A network of inter
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Methods. HE-gefärbte Schnittpräpa
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Conclusions. The newly released 450
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and desmoplastic reaction are diffe
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one patient revealed more than 9 mi
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situation, the V600E mutation in th
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SA-P-064 Evolution of molecular pat
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nic markers such as myf4 and MyoD1
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Conclusions. To our knowledge, this
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SA-P-085 Expression of the eukaryot
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Results. The papillary RCC type II
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SA-P-098 Male infertility: assessme
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SA-P-104 Process oriented scientifi
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Conclusions. The IBDW offers a uniq
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L Lasitschka F. DO-046 Lehmann A. F
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96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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