96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

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Results. The papillary RCC type II were all positive for membranous N-Cadherin staining, whereas type I did not show any membranous positivity for N-Cadherin. The E-Cadherin staining showed a stronger membranous as well as cytoplasmic expression in type II than in type I RCC. A relevant expression of KSP- and P-Cadherin could not be demonstrated. Conclusions. Our data show that all papillary RCC type II are characterized by a membranous N-Cadherin expression. In contrast the papillary RCC type I do not express N-Cadherin membranous at all. Thus N-Cadherin represents the first immunhistochemical marker for a clear differentiation between papillary RCC type I and type II. SA-P-092 Myoglobin expression in renal cell carcinoma is regulated by hypoxia C .L . Behnes1 , J . Bedke2 , A . Strauss3 , F . Bremmer1 , H .-J . Radzun1 1 2 University of Göttingen, Institute of Pathology, University of Tübingen, Institute of Urology, 3University of Göttingen, Institute of Urology Aims. Myoglobin (Mb) is a member of the hemoprotein superfamily, including in addition hemoglobin, neuroglobin and cytoglobin. The functions of the cytoplasmic localized Mb are the prevention of hypoxia and radical scavenging, well known from the myocytes of skletal and myocardic muscles. In case of hypoxia Mb acts as an oxygen reservoir by binding O2 and improving the diffusion of oxygen into the cell. It could be shown that some cancers do express Mb preventing hypoxia. In this study we investigated whether Mb also plays a role in renal cell carcinoma (RCC) and a potential influence of hypoxia on the expression. Methods. Four different RCC cell lines were cultivated under hypoxic conditions and the expression of Mb was evaluated by real-time PCR. For the correlatin between microvessel density and Mb expression tissue microarrys (TMAs) with 42 different RCCs were immunhistochemically stained with a myoglobin- as well as CD31-antibody. Results. We could show that RCC do express Mb. Immunhistochemical examinations of RCC tissues show a reverse relationship between Mbexpression and capillary density. Especially in clear cell RCC a significant relationship between decrease in capillary density and increased expression of Mb could be shown. Furthermore, the expression of Mb in all analyzed RCC cell lines increased under hypoxia up to 60-fold. Conclusions. Mb especially known as a marker for myogenic differentiation is expressed in RCC and RCC cell lines under hypoxia. A significant reverse correlation between capillary density and Mb expression exist in clear cell RCC. Thus, Mb might be a marker for hypovascularized tumor entities/tumor areas. SA-P-093 Impact of early ultrastructural damage after ABO-incompatible and ABO-compatible kidney transplantation V . Broecker1 , A . Pfaffenbach1 , C . Bockmeyer1 , M . Dämmrich1 , S . Immenschuh2 , A . Schwarz3 , H . Kreipe1 , F . Heinemann4 , J .U . Becker1 1 2 Hannover Medical School, Institute for Pathology, Hannover, Hannover Medical School, Institute for Transfusion Medicine, Hannover, 3Hannover Medical School, Department of Nephrology, Hannover, 4Essen University, Institute for Transfusion Medicine, Essen Aims. Following ABO-incompatible kidney transplantation (iABO), c4d in peritubular capillaries (ptc) is frequently positive without further indicators of humoral rejection. Using electron microscopy (EM), we investigated the presence and prognostic relevance of early endothelial damage to glomerular and ptc in transplant kidneys with c4d positivity under different circumstances. Methods. In 20 iABO patients (57 biopsies), 16 ABO-compatible (cABO) patients (26 biopsies with c4d-positivity of ptc) and 14 transplant patients (14 biopsies) without c4d and signs of humoral rejection (controls) were included. 10 different EM parameters were semiquantitatively evaluated: loss of foot processes (FF), lamina rara interna widening (LRI), swelling of glomerular and ptc endothelial cells (GES and ptcES), inflammatory cell adhesion to glomerular and ptc endothelial cells (GIS and ptcIS), glomerular basement membrane double contours (DK), glomerular and ptc basement membrane lamellation (LG and Lptc), loss of glomerular endothelial fenestration (FE). Status of donor specific antibodies (DSA) was available for 35/50 patients. Kidney function (GFR) at biopsy and follow up (51.8±31.74 months) was available for all patients. Results. 3/10 EM parameters were significantly less severe in iABO versus cABO (FF, FE, ptcES); 1/10 significantly more severe in cABO versus controls (FE). No differences were seen between iABO and controls. GFR at biopsy and follow up was significantly lower in cABO versus iABO, although the decline (GFR/time) was not different. GFR at biopsy and follow up correlated with 2/10 EM parameters in iABO (LRI, FE), 2/10 in cABO (GIS, FF). None of the EM parameters correlated with GFR decline. Patients with positive DSA (6/35) had significantly lower GFR at biopsy and follow up, although, GFR decline was not different between DSA positive and negative patients. The presence of DSA correlated with 6/10 EM parameters (FF, LRI, GES, ptcES, DK, FE). Conclusions. Long term outcome in iABO patients is favourable despite c4d positivity of ptc. In this context the value of EM in the detection of early endothelial damage, although more pronounced in cABO patients with signs of humoral rejection, is limited and lacks prognostic relevance. In contrast, the presence of DSA correlates with ultrastructural signs of endothelial alteration and is associated with impaired kidney function. SA-P-094 Large scale in vivo isolation of glomerular podocytes by cationic colloidal silica-coated ferromagnetic nanoparticles A . Blutke1 , R . Wanke1 1Ludwig-Maximilians-University Munich, Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, München Aims. Podocyte homeostasis plays a crucial role for maintenance of the physiological glomerular function and podocyte injury is regarded as a major determinant of development and progression of various renal diseases. Since cultured podocytes cannot completely reflect the complex properties of podocytes in the glomerular environment in vivo, analyses of the molecular processes occurring during podocyte development and injury require appropriate methods for preparation of fresh podocyte isolates. Methods. A novel, fast, antibody-free and most cost-efficient method for reproducible large scale isolation of fresh podocytes from mouse kidney glomeruli is described. Briefly, cationic silica-coated colloidal ferromagnetic nanoparticles were utilized to bind to negatively charged cell surfaces of podocytes in preparations of isolated glomeruli. After enzymatic and mechanical dissociation of the glomerular cells, nanoparticle-coated podocytes were isolated in a magnetic field. Results. Podocytes isolated with this method displayed characteristic phenotypical and ultrastructural features. Protein and mRNA expression abundances of marker-molecules of podocytes, endothelial or mesangial glomerular cells indicated a significant enrichment of podocytes in the generated isolates. Conclusions. The described method offers a great potential for different downstream transcript- and protein profiling analysis technologies, which might contribute to an improved understanding of podocyte biology and of the molecular mechanisms involved in podocyte injury in vivo. Der Pathologe · Supplement 1 · 2012 | 167
Abstracts SA-P-095 Can fibrils induce a humoral immune reaction? A case report and review of literature S . Porubsky 1 , C . Boldt 2 , V . Kliem 2 , H .-J . Gröne 1 1 DKFZ Heidelberg, Heidelberg, 2 Nephrologisches Zentrum Niedersachsen, Hann . Münden Aims. Fibrillary glomerulonephritis is a rare disease of later adulthood and is characterized by randomly arranged Congo-red negative fibrillary deposits with a diameter of 10–20 nm in glomeruli. Due to sclerosis of glomeruli and tubulointerstitium the disease leads usually to chronic kidney failure. We describe a 50-year-old female patient whose medical history was negative with exception of smoking and a febrile bronchitis a few days before admission. The patient presented with proteinuria (3.8 g/d), active urine sediment, hypertension and acute kidney failure. The laboratory findings revealed solely an ANA-titer of 1:160. ANCA- and ASLO-titers as well as hantavirus serology were negative. Complement levels were normal. Methods. Light-microscopical, immunhistochemical and ultrastructural investigations of the kidney biopsy and correlation of the findings with the current literature. Results. The biopsy showed 15 glomeruli of which 5 were characterized by a segmental necrosis and extracapillary proliferation. 2 glomeruli showed a global and 4 a segmental sclerosis. There was moderately severe chronic interstitial damage. Immunohistochemically IgG, IgA, IgM C1q and C3 depositions could be detected in mesangium and along the basement membrane of all glomeruli. Congo-red stain was repeatedly negative. In mesangium and within the glomerular basement membrane, electron microscopy visualized randomly arranged fibrils with a diameter of 10–20 nm. The diagnosis of a fibrillary glomerulonephritis was made. Conclusions. Although seldom crescents have been observed in idiopathic fibrillary glomerulonephritis, a necrotizing form of this disease has not been described in detail. The simultaneous occurrence – with a “full house” pattern – of immunoglobulin, complement factors and fibrils is usually seen in fibrillary glomerulonephritis. Necrosis points to the potential of fibrillary deposits to induce a pronounced complement activation and to cause a necrotizing lesion. This is contradictory to the current paradigm that fibrils are immunologically inert. Furthermore it demonstrates that glomerulopathies with organized deposits are to be considered upon a diagnosis of necrotizing glomerulonephritis. SA-P-096 Pathology of resolving polyomavirus nephropathy T . Menter1 , M . Mayr2 , H .H . Hirsch3 , M .J . Mihatsch1 , S . Schaub4 , H . Hopfer1 1 2 Institute of Pathology, Basel, Switzerland, Medical Outpatient Department, Basel, Switzerland, 3Institute of Medical Microbiology, Basel, Switzerland, 4Clinic for Transplantation Immunology and Nephrology, Basel, Switzerland Aims. Polyoma virus nephropathy (PVN) is a common complication after renal transplantation, affecting up to 20% of patients. The standard therapy is reduction of immunosuppression, which leads to an effective virus control in more than 90% of cases. So far, the morphology of resolving PVN has not been investigated. We compared the morphological findings in protocol biopsies of PVN patients prior to viremia, during increasing and decreasing viremia as well as after virus clearance. Methods. The study included 101 transplant biopsies of 34 patients transplanted between 2005 and 2010 and diagnosed with PVN which were treated by reduction of immunosuppression only. Biopsies were scored according to Banff-criteria, the extent of inflammation and interstitial fibrosis was estimated as% of renal cortex, and the number of tubular cross sections with SV40+ cells per mm of biopsy length was counted. Biopsy findings were correlated with virus load in the serum and cli- 168 | Der Pathologe · Supplement 1 · 2012 nical follow-up data, and grouped as pre-, increasing, decreasing, and post-viremia. Results. We found a significant increase in interstitial inflammation (median decreasing viremia 10% vs. increasing viremia 0.3%, p
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Inhalt Der Pathologe · Supplement
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Inhalt Der Pathologe · Supplement
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Editorial Liebe Kolleginnen und Kol
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Kolorektales Karzinom 2 VO-005 Tran
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Keynote Lecture VO-014 Genetic dete
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(AMACR, FASN, GOLM1, GSP-pi, ERG) t
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to assess the correct rate of R1 re
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DNA damage, and cytotoxic drugs. Au
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HCV-positive formalin-fixed and par
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well as MET activation were examine
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or BRAF mutation, c-MYC and SIRT1 e
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AG Pneumopathologie III DO-032 Remo
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immature granulopoiesis showed a st
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ned, if well-defined mantle zones,
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phomas). Most prominent gains or am
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DO-062 Tumor-associated macrophages
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DO-080 DOG1: an immunohistochemical
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AG Oralpathologie DO-087 Detection
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DO-094 Do activated fibroblasts inf
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DO-101 Histopathological analysis o
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DO-108 Identification of potential
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Conclusions. In conclusion, 454 par
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subgroup of patients with B-Raf mut
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DO-002b Impact of terminologies in
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Methods. We performed MCPyV-FISH of
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FR-013 HPV-genotype distribution in
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Methods. Three different techniques
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(i.e. investment in equipment and e
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FR-032 COLD-PCR: a powerful tool in
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dissection (MBLND) technique to imp
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SO-005 Prevalence of mutations in s
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SO-011 Methylation profiling and in
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more effective than SAHA alone and
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SO-022 Specialized pathology review
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SO-027 Ki-67 in mitotic score group
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nificantly associated with advanced
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liver did not correlate with the mu
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AG Paidopathologie II SO-046 Overex
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Results. Histomorphology of the ova
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p42 and p27 have not yet been inves
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SO-068 Recent advances in understan
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SO-075 A novel, dual role of CCN3 i
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Pancreatic Adenocarcinoma SG-137 Se
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sease and 30 colon cancer serum sam
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Conclusions. Although CRC is charac
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differentiated and TNM stage III or
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pressed moderate levels of the prot
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FR-P-037 MALDI imaging reveals COX7
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in the context of therapy response
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on primary cells of wild-type and c
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chemotherapy was recommended and co
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aims are twofold: 1) to verify the
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Results. Her2/neu status in TMAs an
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prognostic impact was found in rect
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provided information on survival ti
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Methods. Biopsy specimens from 430
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the OS rate was 53% for patients wi
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FR-P-098 Immunohistological stainin
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Page 128 and 129: FR-P-136 The expression of central
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Page 132 and 133: FR-P-149 Combination of Castleman d
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Page 136 and 137: Results. In 18 cases (79%) the caus
Page 138 and 139: FR-P-168 Autopsy findings in a 2-ye
Page 140 and 141: FR-P-174 MPGN-like glomerulonephrit
Page 142 and 143: Poster: Gynäkopathologie und Mamma
Page 144 and 145: SA-P-011 Genetic aberrations of pre
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Page 148 and 149: internet server. A network of inter
Page 150 and 151: Methods. HE-gefärbte Schnittpräpa
Page 152 and 153: Conclusions. The newly released 450
Page 154 and 155: and desmoplastic reaction are diffe
Page 156 and 157: one patient revealed more than 9 mi
Page 158 and 159: situation, the V600E mutation in th
Page 160 and 161: SA-P-064 Evolution of molecular pat
Page 162 and 163: nic markers such as myf4 and MyoD1
Page 164 and 165: Conclusions. To our knowledge, this
Page 166 and 167: SA-P-085 Expression of the eukaryot
Page 170 and 171: SA-P-098 Male infertility: assessme
Page 172 and 173: SA-P-104 Process oriented scientifi
Page 174 and 175: Conclusions. The IBDW offers a uniq
Page 176 and 177: L Lasitschka F. DO-046 Lehmann A. F
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