96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

Abstracts
Conclusions. The AIM2 expression and induction patterns suggest a role
in vascular pathogenesis. AIM2 might act as a danger signal in vascular
EC, SMC and infiltrating inflammatory cells.
DO-104
Carbamylated EPO-fusion protein and recombinant human EPO
during porcine kidney I/R injury
F . Simon1 , M . Gröger2 , O . McCook2 , E . Calcia2 , P . Radermacher2 , H . Schelzig1 1 2 University of Düsseldorf, University of Ulm, Ulm
Aims. A newly carbamylated erythropoietin-fusion protein (cEPO-FC)
and recombinant human EPO (rhEPO) equally protected against spinal
cord I/R injury in young, healthy swine [1]. In a recent clinical trial,
however, rh-EPO did not affect acute kidney injury in ICU patients [2].
Since patients often present with vascular disease and consecutive organ
dysfunction, we compared cEPO-FC and rh-EPO in swine with ubiquitous
atherosclerosis [3].
Methods. Pigs randomly received either of cEPO-FC (50 μg/kg), rh-EPO
(5000 IU/kg) or vehicle twice over 30 min before and during the first
4 h of reperfusion after 120 min of aortic occlusion using inflatable balloons.
We assessed creatinine-clearance, fractional Na+ excretion, blood
NGAL, cytokine and NO levels together with tissue histology and immune-histochemistry
and -blotting for iNOS, HO-1, HIF-1α , NF-κB,
and markers of apoptosis.
Results. All pigs presented with reduced glomerular filtration (creatinine-clearance
74±24 vs. 90–140 mL/min normal value) and pre-existing
histological organ damage. Neither cEPO-FC nor rh-EPO beneficially
influenced the I/R-induced kidney dysfunction, histological organ damage
nor tissue inflammation and apoptosis.
Conclusions. Pre-existing atherosclerosis-induced kidney dysfunction
and tissue damage may reduce the efficacy of cEPO-FC and rh-EPO to
prevent I/R-induced kidney damage.
References
1 . Simon F et al (2011) . Intensive Care Med, in press
2 . Thim T et al (2010) . EuroIntervention 6:261–8
3 . Endre Z et al (2010) . Kidney Int 77:1020–30
DO-106
Antibody-mediated rejection in cardiac transplant recipients is a
seasonal disease
K . Wassilew1 , N .E . Hiemann1 , D . Kemper1 , R . Hetzer1 1Deutsches Herzzentrum Berlin, Berlin
Aims. Diagnosis of antibody-mediated rejection (AMR) is still a matter of
controversial discussion. We suggested that staining for immunoglobulins
might improve the diagnostic spectrum of AMR because of seasonal
effects in complement deposition.
Methods. We studied prospectively all endomyocardial biopsies harvested
since 01/2011 (n=205) for acute cellular rejection, activated endothelial-cells
and deposition of C4d, C3d and IgA/M/G in interstitial
capillaries by paraffin immunohistochemistry. Histologic and immunohistochemical
parameters of AMR were classified according to the
ISHLT and studied for seasonal effects in an ordinal (Jan–Mar vs. Apr–
Jun vs. Jul–Sept vs. Oct–Dec) and nominal model (Oct–Mar vs. Apr–
Sept).
Results. Overall, 16% of biopsies showed signs of acute cellular rejection
of any grade and 46% of samples showed evidence of endothelial-cell
swelling. In the ordinal model (Jan–Mar vs. Apr–Jun vs. Jul–Sep vs.
Oct–Dec), seasonal effects were found for endothelial cell swelling (83%
vs. 14% vs. 49% vs. 40%; p